Phytochemical Nature and Pharmacological Evaluation of Chloroform Extract of Pandanus fascicularis L. (Fruits) An in vivo Study

The present study was conducted to detect possible chemicals (phytoconstituents), and investigate antioxidant, antimicrobial and thrombolytic activities of the extract of Aphanamixis polystachya (stem bark). Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in Vitro antimicrobial activity, disc diffusion method, and to determine the thrombolytic activity, the method of Prasad et al., 2007 with minor modifications were used. The bark extracts were a rich source of phytochemicals. In DPPH free radical scavenging test, the Carbon tetra chloride soluble fraction showed the highest free radical scavenging activity with IC50 value 19.86 µg/ml. while compared to that of the reference standards ascorbic acid. Aphanamixis polystachya was also found as a good source of total phenolic contents. Moreover, the extracts revealed broad spectrum antimicrobial activity at the concentration of 400 µg/disc. By comparing with the negative control the mean clot lysis % was significant (p value <0.0009). Therefore, further studies are suggested to determine the active compounds responsible for the biological activities of the plant extracts.

Section: Introductionmentioning

confidence: 99%

A Study on Aphanamixis polystachya for evaluation of phytochemical and pharmacological properties

Kobir¹,

Dewanjee²,

Hossain³

et al. 2022

“…Nature provides different remedies for human being from its plants, animals and other sources to treat all ailments of mankind [1,2]. Medicinal plants are important contributors among all natural sources [3].…”

Section: Introductionmentioning

confidence: 99%

“…They always play an important role in the development of health in mankind [4]. According to World Health Organization (WHO), about 80% of the world population still uses medicinal plants for different medical purposes [2]. Even in recent time, people are still taking traditional medicine for their primary health care [3].…”

Section: Introductionmentioning

confidence: 99%

Characterization of phytochemicals and determination of antioxidant, antimicrobial and cytotoxic properties of the medicinal plant Ardisia solanacea

Hossain¹,

Dewanjee²,

Islam³

et al. 2022

The present study was conducted to detect possible phytochemicals and evaluate antioxidant, antimicrobial and cytotoxic activities of the extract of Ardisia solanacea. Phytochemical screening was carried out using the standard test methods of different chemical group. For investigating the antioxidant activity, two complementary test methods namely DPPH free radical scavenging assay and total phenolic content determination were carried out. For the evaluation of in vitro antimicrobial activity, disc diffusion method was used. Evaluation of cytotoxic activity was done using the brine shrimp lethality bioassay. In DPPH free radical scavenging test, the petroleum ether soluble fraction showed the highest free radical scavenging activity with IC50 value 40.04 μg/ml. while compared to that of the reference standards ascorbic acid. Ardisia solanacea was also found as a good source of total phenolic contents. Moreover, the extracts revealed moderate antimicrobial activity at the concentration of 400 μg/disc. In cytotoxic activity test, the petroleum ether soluble fraction showed significant cytotoxic potential (LC50 value of 0.703 μg/ml) among all the fractions comparing with that of standard vincristine (0.544 μg/ml). Therefore, further studies are suggested to determine the active compounds responsible for the pharmacological activities of the plant extracts.

“…Production of ROS and free radicals is caused due to aerobic metabolism and it is must for normal functioning of the human body [7]. From study it is found that excess level of free radical cause several diseases including: cancer, diabetes, cardiovascular and neurodegenerative diseases [8].…”

Section: Introductionmentioning

confidence: 99%

In vitro cytotoxic, thrombolytic, anthelmintic and antioxidant activities of Litsea monopetala: A medicinal plant

Dewanjee¹,

Akter²,

Hossain³

et al. 2022

The present study was conducted to assess the cytotoxicity, thrombolytic, anthelmintic and antioxidant activity of mehanolic extract of Litsea monopetala (Family: Lauraceae) leaves in laboratory using in vitro methods. Cytotoxicity test was done by brine shrimp lethality bioassay where the extract concentration was 400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125 (µg/ml). In vitro thrombolytic activity of Litsea monopetala was performed by clot lysis method using extract concentration 2.5, 5, 10 and 20 (mg/mL) in saline water. Anthelmintic activity test was done by using adult earthworms where 10, 20, 40, 60, 80 (mg/ml) extract concentration were used. Finally antioxidant activity was determined by total phenolic content determination using Folin-Ciocalteu reagent. The Litsea monopetala extract showed cytotoxic activity against brine shrimp nauplii and LC50 value was 41.05(µg/ml) and the investigated thrombolytic activity in our research was 9.52, 9.49, 13.64 and 17.50 % respectively as % of clot lysis. The paralysis time were at 76.75 min, 60 min, 51.75 min, 44.5 min and 64.5 min and death were at 90.50min, 63.75min, 55.50min, 44.75min and 71min. respectively. The Litsea monopetala extract displayed significant antioxidant activity which was 20.75 (mg of GAE / gm) of extracts. The activities observed could be attributed to presence of some of the phytochemicals which have been related with cytotoxic, thrombolytic, anthelmintic and antioxidant property.