Phytochemical profile and antioxidant capacity, α-amylase and α-glucosidase inhibitory activities of Oxalis pes-caprae extracts in alloxan-induced diabetic mice

Kabach, Imad; Bouchmaa, Najat; Zouaoui, Zakia; Ennoury, Abdelhamid; Asri, Sara El; Oumeslakht, Loubna; Cacciola, Francesco; Majdoub, Yassine Oulad El; Mondello, Luigi; Zyad, Abdelmajid; Nhiri, Naima; Nhiri, Mohamed; Mrid, Reda Ben

doi:10.1016/j.biopha.2023.114393

Cited by 25 publications

(14 citation statements)

References 66 publications

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“…However, it should be noted that the in vitro results showed higher α-glucosidase inhibitory activity compared with the in vivo antidiabetic effect. This finding is consistent with the results of other studies where compounds or extracts with high activity in in vitro assays showed lower activity in in vivo studies, possibly owing to differences in their absorption, distribution and metabolism, as well as the complex pathophysiology of DM (Kabach et al, 2003;Oboh et al, 2019;Srinivasa et al, 2023).…”

Section: Discussionsupporting

confidence: 92%

Evaluation of antidiabetic and antioxidant effects of Polygonum cognatum Meisn. and phytochemical analysis of effective extracts

Gözcü,

Uğan,

Özbek

et al. 2023

Biomedical Chromatography

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Polygonum cognatum Meisn. (Polygonaceae) is used both as food and as a folk medicine to treat diabetes. This study aimed to evaluate the effect of the extracts, along with isolated compounds, from P. cognatum aerial parts on diabetes. In vitro studies were conducted using an α‐glucosidase inhibitory assay, while in vivo antidiabetic studies were carried out on streptozotocin‐induced diabetic rats. Effective extracts were subjected to isolation studies, and structures of the compounds were elucidated by spectroscopic methods. The ethyl acetate and n‐butanol extracts had the highest effect in both in vitro and in vivo experiments. They also decreased aspartate transaminase, alanine transaminase and malondialdehyde levels, while increasing glutathione and superoxide dismutase activity in rats. From the active extracts, 11 phenolic compounds were isolated and characterized. Among the isolated compounds, quercetin was found to be the most active according to α‐glucosidase inhibitory activity studies. This study provided scientific evidence for the traditional use of P. cognatum as a folk medicine for treating diabetes. The findings suggest that the ethyl acetate and n‐butanol extracts, as well as quercetin, have the potential for development as antidiabetic agents.

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Section: Discussionsupporting

confidence: 92%

Evaluation of antidiabetic and antioxidant effects of Polygonum cognatum Meisn. and phytochemical analysis of effective extracts

Gözcü,

Uğan,

Özbek

et al. 2023

Biomedical Chromatography

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“…The digestion of carbohydrates is highly dependent on the activity of α‐amylase and α‐glucosidase (Kabach et al ., 2023). α‐amylase has the ability to break down starch by hydrolysing α‐1,4 glycosidic bonds, resulting in the formation of disaccharides or oligosaccharides (Zhang et al ., 2023).…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress is one of the main mechanisms leading to diabetes and its complications (Kabach et al, 2023). In order to promote the health of diabetic patients clinically, some dietary supplements containing antioxidants will be provided to patients (Purwaningsih et al, 2023).…”

Section: Determination Of Antioxidant Activity Of E Braceanus Fruits ...mentioning

confidence: 99%

“…Phenolics are common natural compounds synthesised by plants through secondary metabolism (de Araujo et al ., 2021). Further research has revealed that phenolics possess potential benefits in the prevention and intervention of diabetes (Chen et al ., 2021; Kabach et al ., 2023), as they exhibit not only excellent antioxidant activities but also considerable inhibitory effects on α‐amylase and α‐glucosidase (Zheng et al ., 2020). Therefore, it is imperative to investigate natural phenolics and elucidate their therapeutic effects and mechanisms in the management of diabetes.…”

Section: Introductionmentioning

confidence: 99%

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Exploring antioxidant activities and inhibitory effects against α‐amylase and α‐glucosidase of Elaeocarpus braceanus fruits: insights into mechanisms by molecular docking and molecular dynamics

Li,

Zhang,

Liu

et al. 2023

Int J of Food Sci Tech

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SummaryThe present work investigated the antioxidant activity and inhibitory effects towards α‐amylase and α‐glucosidase of phenolic compounds in Elaeocarpus braceanus fruits and clarified their possible mechanisms based on molecular docking and dynamic analysis. A total of 12 phenolic compounds were identified and quantified in the extracts of E. braceanus fruits using UHPLC‐ESI‐HRMS/MS. The TPC and TFC were 229.18 ± 5.76 mg GAE/g and 144.85 ± 1.17 mg RE/g, respectively. In vitro analyses, E. braceanus fruits extracts exhibited good antioxidant activities and demonstrated strong inhibitory effects towards α‐amylase and α‐glucosidase. Molecular docking and molecular dynamics results showed that quercetin and myricetin can stably form hydrogen bonds and van der Waals forces with the active amino acid residues of α‐amylase and α‐glucosidase, and thereby inhibiting their enzymatic activities. The findings might provide a good potential natural resource for improving oxidative stress damage and controlling blood glucose in diabetes.

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“…The potential inhibitory activity of the C.spicatus plant extract against α-amylase (Sigma-Aldrich, United States) was assessed using a speci c protocol. (23). The C.spicatus leaf extract (100 µL prepared in 3% dimethyl sulfoxide) was added to 100 µL of α-amylase solution (0.1 mL prepared in pH 6.9 phosphate buffer solution) and incubated at 37°C for 30 minutes.…”

Section: α-Amylase Inhibitory Assaymentioning

confidence: 99%

Phytochemical profiling and In-vitro antioxidant activity of In-vivo α- amylase and α- glucosidase inhibitory activities of kidney Costus spicatus extract in diabetic Albino wistar Rats

Sivalingam

Pandian

Rengarajan

et al. 2023

Preprint

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The study found that C. spicatus extract had a beneficial effect on body weight and blood glucose levels in diabetic rats. Additionally, the extract contained polyphenol compounds with antioxidant, enzyme inhibition, and antiglycation activity. The blood glucose levels of the experimental groups were measured over a 28-day period, and the lipid profile was also assessed. The important result of this study is that the plant extract from C.spicatus was able to recover the antioxidant activity parameters, as well as improve the activity of glycolysis enzymes in the kidney of diabetic rats. This suggests that the plant extract has potential as a natural remedy for diabetes and its complications. Diabetic control + C.spicatus 500 mg/kg at 0.99 ± 0.05ab, Pyruvate kinase as a normal control at 20.01 ± 0.05a and 14.41 ± 1.2c, and Glucose-6-Phosphatase Dehydrogenase as a normal control at 0.52 ± 0.02a and Diabetic control + C.spicatus 500 mg/kg at 0.66 ± 0.05c. The study found that the C. spicatus leaf extract was effective in improving the antioxidant activity of important parameters such as SOD, CAT, GPx, and GR, and protected against hyperglycemia and oxidative damage in type 2 diabetes-induced hepatorenal functions both in vitro and in vivo. The kidney glycolysis enzymes, Hexokinase, Pyruvate kinase, and Glucose-6-Phosphatase Dehydrogenase, also showed improvement in the experimental group. Overall, the C. spicatus leaf extract proved to be effective in protecting against hyperglycemia and oxidative damage in type 2 diabetes-induced hepatorenal functions in both in vitro and in vivo studies.

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Phytochemical profile and antioxidant capacity, α-amylase and α-glucosidase inhibitory activities of Oxalis pes-caprae extracts in alloxan-induced diabetic mice

Cited by 25 publications

References 66 publications

Evaluation of antidiabetic and antioxidant effects of Polygonum cognatum Meisn. and phytochemical analysis of effective extracts

Evaluation of antidiabetic and antioxidant effects of Polygonum cognatum Meisn. and phytochemical analysis of effective extracts

Exploring antioxidant activities and inhibitory effects against α‐amylase and α‐glucosidase of Elaeocarpus braceanus fruits: insights into mechanisms by molecular docking and molecular dynamics

Phytochemical profiling and In-vitro antioxidant activity of In-vivo α- amylase and α- glucosidase inhibitory activities of kidney Costus spicatus extract in diabetic Albino wistar Rats

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