Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Angeloni, Simone; Maggi, Filippo; Sagratini, Gianni; Caprioli, Giovanni; Borsetta, Germana; Ak, Güneş; Sinan, Kouadio Ibrahime; Zengin, Gökhan; Arpini, Sabrina; Mombelli, Giacomo; Ricciutelli, Massimo

doi:10.3390/plants10020195

Cited by 23 publications

(23 citation statements)

References 43 publications

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“…The free radical scavenging activity of oat extract was determined using a 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay. Phenolic components in plant foods such as barley [ 34 ] and Leonurus cardiaca [ 35 ] are reported to be correlated with their radical scavenging activities. The DPPH radical scavenging activities of water, the 40% ethanol and the 80% ethanol extracts of Daeyang and Choyang cultivars are presented in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

Oat (Avena sativa) Extract against Oxidative Stress-Induced Apoptosis in Human Keratinocytes

Song

Lee

et al. 2021

Molecules

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Oat (Avena sativa) is well known for its various health benefits. The protective effect of oat extract against oxidative stress-induced apoptosis in human keratinocytes HaCaT was determined. First, extracts of two varieties of oat, Daeyang and Choyang, were analyzed for fat-soluble antioxidants such as α-tocotrienol, γ-oryzanols, lutein and zeaxanthin using an UPLC system and for antioxidant activity using a DPPH assay. Specifically, an 80% ethanol extract of Daeyang oat (Avena sativa cv. Daeyang), which had high amounts of antioxidants and potent radical scavenging activity, was further evaluated for protective effect against oxidative stress-induced cell death, intracellular reactive oxygen species levels, the phosphorylation of DNA damage mediating genes such as H2AX, checkpoint kinase 1 and 2, and p53 and the activation of apoptotic genes such as cleaved caspase-3 and 7 and poly (ADP-ribose) polymerase in HaCaT cells. The Daeyang and Choyang oat 80% ethanol extracts had 26.9 and 24.1 mg/100 g γ-oryzanols, 7.69 and 8.38 mg/100 g α-tocotrienol, 1.25 and 0.34 mg/100 g of lutein and 1.20 and 0.17 mg/100 g of zeaxanthin, respectively. The oat 80% ethanol extract treatment (Avena sativa cv. Daeyang) had a protective effect on oxidative stress-induced cell death in HaCaT cells. In addition, the oat 80% ethanol extracts led to a significant decrease in the intracellular ROS level at a concentration of 50–200 μg/mL, the attenuation of DNA damage mediating genes and the inhibition of apoptotic caspase activities in a dose dependent manner (50–200 μg/mL). Thus, the current study indicates that an oat (Avena sativa cv. Daeyang) extract rich in antioxidants, such as polyphenols, avenanthramides, γ-oryzanols, tocotrienols and carotenoids, has a protective role against oxidative stress-induced keratinocyte injuries and that oat may a useful source for oxidative stress-associated skin damage.

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Section: Resultsmentioning

confidence: 99%

Oat (Avena sativa) Extract against Oxidative Stress-Induced Apoptosis in Human Keratinocytes

Song

Lee

et al. 2021

Molecules

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“…Previous studies of its use as an anti‐inflammatory can be found in the scientific literature; [19,28] however, its usage for wound healing is slightly related [29] . In several extracts of Leonurus species, different secondary metabolites are present, the flavonoids being the main metabolites (e. g. flavonoids such as quercetin and derivatives) [23,30] . In order to confirm the presence of these main compounds (flavonoids) in the studied extracts (hydroalcoholic and aqueous), the total flavonoids were initially determined.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Evaluation of Wound Healing Potential ofLeonurus japonicusHoutt. Extracts

de Assunção Morais,

Koga,

Klein

et al. 2023

Chemistry & Biodiversity

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Leonurus japonicus Houtt. is a medicinal plant popular in Brazil as “rubim”, used in local folk medicine for several applications as an anti‐inflammatory, antioxidant, analgesic, and antimicrobial phytomedicine. The traditional use for wound healing is related; however, few studies have evaluated the wound healing activity. Thus, this study aimed to analyse the popular indication of the hydroalcoholic and aqueous extracts of L. japonicus aerial parts in a rat wound healing model. The initial chemical characterization was performed using flavonoid quantification and complemented with mass spectroscopy/chemometrics analysis. The wound‘s lesion contraction and tissue regeneration (histological study stained with hematoxylin‐eosin and picrosirius) were determined. Hydroalcoholic and aqueous extracts presented high flavonoid content, and mass spectrometry analysis of the extracts demonstrated the presence of compounds with a mass between 100–650, reinforcing the presence of polyphenolic constituents. The extracts of L. japonicus improve various wound healing phases, like inflammatory modulation, wound contraction, and collagen synthesis, resulting in faster healing in rats. These effects could be related to the extracts′ polyphenolic compounds.

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“…Hydroxybenzoic (p-hydroxybenzoic, vanillic) and hydroxycinnamic (chlorogenic, caffeic, caffeoylmalic, and trans-ferulic) acids, flavonoids (rutin and quercetin), phenylethanoid glycosides (verbascoside and lavandulifolioside) [66] Table 1. Cont.…”

Section: Medicinal Plantmentioning

confidence: 99%

Electrochemical Characterization of the Antioxidant Properties of Medicinal Plants and Products: A Review

Ziyatdinova

Kalmykova

2023

Molecules

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Medicinal plants are an important source of bioactive compounds with a wide spectrum of practically useful properties. Various types of antioxidants synthesized in plants are the reasons for their application in medicine, phytotherapy, and aromatherapy. Therefore, reliable, simple, cost-effective, eco-friendly, and rapid methods for the evaluation of antioxidant properties of medicinal plants and products on their basis are required. Electrochemical methods based on electron transfer reactions are promising tools to solve this problem. Total antioxidant parameters and individual antioxidant quantification can be achieved using suitable electrochemical techniques. The analytical capabilities of constant-current coulometry, potentiometry, various types of voltammetry, and chrono methods in the evaluation of total antioxidant parameters of medicinal plants and plant-derived products are presented. The advantages and limitations of methods in comparison to each other and traditional spectroscopic methods are discussed. The possibility to use electrochemical detection of the antioxidants via reactions with oxidants or radicals (N- and O-centered) in solution, with stable radicals immobilized on the electrode surface, via oxidation of antioxidants on a suitable electrode, allows the study of various mechanisms of antioxidant actions occurring in living systems. Attention is also paid to the individual or simultaneous electrochemical determination of antioxidants in medicinal plants using chemically modified electrodes.

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Phytochemical Profile and Biological Activities of Crude and Purified Leonurus cardiaca Extracts

Cited by 23 publications

References 43 publications

Oat (Avena sativa) Extract against Oxidative Stress-Induced Apoptosis in Human Keratinocytes

Oat (Avena sativa) Extract against Oxidative Stress-Induced Apoptosis in Human Keratinocytes

Preliminary Evaluation of Wound Healing Potential ofLeonurus japonicusHoutt. Extracts

Electrochemical Characterization of the Antioxidant Properties of Medicinal Plants and Products: A Review

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