Phytochemical profile and rosmarinic acid purification from two Peruvian Lepechinia Willd. species (Salviinae, Mentheae, Lamiaceae)

Abstract

The phytochemical profile of Lepechinia meyenii (Walp.) Epling and Lepechina floribunda (Benth.) Epling obtained by liquid chromatography associated with high-resolution mass spectrometry is presented. Forty eight compounds were detected exhibiting a variety of salvianolic acids and abietane phenolic diterpenoids. A simple procedure by cold evaporative crystallization to purify rosmarinic acid from these botanical species was also shown.

Phytochemical profile of eleven peruvian Mentheae. Isolation of ursolic acid from Clinopodium revolutum (Ruiz & Pavon) Govaerts and analysis of the aqueous infusions

“…This type of substances is very important due to its effect on cell brosis (scar formation) in direct relation to cancer [42]. Among the other substances found, it should be noted that the Rosmarinus type diterpenoids, common in Lepechinia [7,43] are scarce in this work, only Salvia sagitatta and Salvia cuspidata show the presence of carnosol (m/z 285) and the phenolic diterpenoid, rosmadial (m/z 343) in the last one. Salvia dombeyi turns out to be the most dissimilar plant in this work, since apart from a very low content of rosmarinic acid [6] and the presence of polymethoxylated avones, it shows substances such as cimifugin (m/z 305), a substance with antiallergic and antipruritic activity [51,52] and the lignan lariciresinol (m/z 359), common in oilseeds and in the Brassica and Allium genera [53].…”

Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling

“…L. meyenii, including caffeic acid (1), hesperidin (2), diosmin (4), methyl rosmarinate ( 5), diosmetin (6), and butyl rosmarinate (7), among which caffeic acid, rosmarinic acid, and methyl rosmarinate have previously been reported in L. meyenii [16,39]. In contrast, hesperidin, diosmin, diosmetin, and butyl rosmarinate were identified in L. meyenii for the first time in this study.…”

“…Rosmarinic acid (3) was identified as the key contributor to the antioxidant and AR inhibitory activities of L. meyenii, as the rosmarinic acid content (mg/g) showed a strong positive and significant correlation with the activities against DPPH radicals (r = 0.945, p < 0.001) and AR (r = 0.923, p < 0.001) in the extracts/fraction of L. meyenii (Figure 7). In addition to rosmarinic acid (3), six more compounds were separated and identified from L. meyenii, including caffeic acid (1), hesperidin (2), diosmin (4), methyl rosmarinate (5), diosmetin (6), and butyl rosmarinate (7), among which caffeic acid, rosmarinic acid, and methyl rosmarinate have previously been reported in L. meyenii [16,39]. In contrast, hesperidin, diosmin, diosmetin, and butyl rosmarinate were identified in L. meyenii for the first time in this study.…”

Zostera marina L.: Supercritical CO2-Extraction and Mass Spectrometric Characterization of Chemical Constituents Recovered from Seagrass

“…It was identified in the bibliography in extract from Mentha [17]; Lamiaceae spp. [18]; Lepechinia [19]. The CID spectrum in positive ion mode of hydroxycoumarin Umbelliferone from Z. marina is shown in Figure 12. [M+H] + ion produced one fragment ion with m/z 144.99 (Figure 12).…”

“…It was identified in the bibliography in extract from Mentha [17]; Lamiaceae spp. [18]; Lepechinia [19].…”