Phytochemical Profile And Toxicity Testing Of African Leaf Extract (Vernonia Amygdalina Delile) With Liquid Variation Scavenger Method Brine Shrimp Lethality Test (BSLT)

Abstract

Background: The African leaf (Vernonia amygdalina Delile) is one of the plants used as a traditional medicine, one of which is anti-cancer. Aim: The study aims to identify compound groups, toxic effects and LC50 values of African leaf extracts with variations in the leaf fluid. Method: In this study, African leaves were extracted using a maseration method with three fluids namely n-hexane, ethyl acetate and 70% ethanol. The extract was qualitatively studied by the group of chemical compounds and toxicity tests were conducted using the method of Brine Shrimp Lethality Test. (BSLT). Result: The results of this study showed that n-hexane extract contains flavonoid and glycoside compounds. Ethyl acetate extract has a flavonoid compound, saponins and glucosides. The results of a linear regression analysis showed that 70% ethanol extract was an extract with the highest LC50value with a LC50 value of 31.80 ppm, an ethyl acetate extracts with LC50 values of 51.70 ppm and n-hexane extracts with LC50, with a 91.88 ppm value of LC50. Conclusion: This study showed that the three extracts included a strong toxic ketegori with a range of 0-100 ppm.

Physicochemical Determination Of Ethyl Acetate Extract And N-Hexane Extract Of Azadiractha Indica A. Juss Leaves

“…The top of each filtrate was taken and tested with Mayer, Wagner, and Dragendorf reagents. The formation of orange, brown and white precipitates indicates alkaloids [8].…”