Phytochemical Profile, Antioxidant Activity of Leaf of Hoslundia opposita Vahl and Docking of its Phytochemical Components with Uropathogenic Strain F11(ID:3NRP) and NFeoB from Escherichia coli BL21(ID:5FH9)

Abstract

Hoslundia opposita Vahl is a multi-purpose medicinal plant used traditionally to treat gonorrhea, cystitis, cough, fever, snake bites, convulsion in many parts of Africa. The objectives of this research are to evaluate the phytochemical profile, antioxidant activity of the leaves of Hoslundia opposita Vahl and to dock its phytochemical components with uropathogenic Escherichia coli strain F11(ID:3NRP) and NFeoB from Escherichia coli BL21(ID:5FH9). Harborne’s method was used for the identification of the class of phytochemicals while GC-MS was used to identify the type of phytochemicals. Folin-Ciocalteau method was used to determine the total phenolic content while aluminum colorimetric assay was used to estimate the total flavonoid content. 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was used to evaluate the antioxidant activity. The molecular docking simulation method was used to investigate the interactions of the phytochemicals with the Escherichia coli receptors. In the phytochemical studies, flavonoids, terpenoids, cardiac glycosides and saponins were identified to be present in the methanolic leaf extract while steroids, alkaloids and anthraquinones were absent. The GC-MS analysis of the methanolic extract revealed presence of 17 compounds out of which 14 were identified. The compounds with appreciable quantity in the leaf extract were 1, 2, 3-benzetriol (38.11%), n-hexadacanoic acid (13.52%) and catechol (9.98%). Assessment of the antioxidant activity using DPPH gave a scavenging activity of 79.86% at 500ug/ml compared with ascorbic acid having a scavenging activity of 90.85% at the same concentration. Evaluation of the total phenolic and flavonoid contents at 500 ug/ml gave the values of 262.54 mg gallic acid equivalent/g extract and 6.24 mg quercetin equivalent/g extract respectively compared with gallic acid and quercetin with maximum concentrations of 385.12 and 12.46 respectively. This shows that the extract has significant antioxidant activity and can be explored as a valuable source of natural antioxidants. GC-MS analysis of the methanolic extract of Hoslundia opposita showed the presence of trans-3-(trifluoromethyl)cinnamic acid, octadecyl ester which was active against Escherichia coli. Docking results with 3NRP and 5FH9 showed binding affinities of -6.1, -6.6, 7.2and -7.0,7.4, 8.5 kJ/mol with the commercial drugs : ciprofloxacin, levofloxacin and tetracycline respectively while that of trans 3-(trifluoromethyl)cinnamic acid, octadecyl ester identified in the leaves were 5.6 and 5.9 kJ/mol.

Determination of total phenolic and flavonoid contents, antioxidant and antibacterial potential of the bark extracts of Syzygium guineense (Wild.) DC

“…This is in agreement with previous studies in that different parts of the plant extracted with more polar solvents showed high TPC and TFC [24]. The TPC and TFC reported in the present study were found to be high when compared to the reports elsewhere [11,35]. The antioxidant properties are correlated with the phenolic content of any plants which act as hydrogen donors, reducing agents, and are capable of scavenging free radicals [36,37].…”

Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing

“…In Uganda, the identified compounds in essential oils of this plant that have also been found in our study were; germacrene D, phytol and palmitic acid [64]. Edewor et., al [65] identified compounds from the methanolic extract of H.opposita Vahl from Nigeria and found 17 compounds of which Hexadecanoic acid, methyl ester, n-hexadecanoic acid, Octadecanoic acid were also identified in this study. However, the same authors did not find alkaloids and tannins in the quantitative phytochemistry which is opposed to this study.…”

Effect of <i>Syzygium guineense</i> and <i>Borassus aethiopum</i> leaves on protein glycation and oxidative stress suppression

“…Traditionally, the plant parts have beenu sed for the treatment of menstrual cycle disorder (Nigatu, 2004), constipation, diarrhea, dysentery (Kisangau et al, 2007), arthritis, rheumatism, venereal diseases, malaria (Kasali et al, 2014), sleep disorder, anaemia (Nguyen et al, 2016), diabetes mellitus, microbial and fungi infections (Ezenyi et al, 2016). Additionally, scientific investigations have demonstrated that the methanol and aqueous extracts of the plant leaves possess antioxidant effect (Pieme et al, 2014;Edewor et al, 2021). However, the antioxidant activity of the ethylacetate and chloroform extracts of the plant leaves together with the antiglycation potential of these extracts remain a knowledge lacuna that needs to be bridged.…”