Phytochemical Profile of the Aerial Parts of <i>Sedum sediforme</i> and Anti-inflammatory Activity of Myricitrin

Winekenstädde, Daniel; Angelis, Apostolis; Waltenberger, Birgit; Schwaiger, Stefan; Tchoumtchoua, Job; König, Stefanie; Werz, Oliver; Aligiannis, Nektarios; Skaltsounis, Alexios‐Léandros; Stuppner, Hermann

doi:10.1177/1934578x1501000122

Cited by 8 publications

(4 citation statements)

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“…However, at the concentrations corresponding to the IC 50 value calculated after exposure to the extract obtained of S. senegalensis leaves, only 7 proved to be active, leading to a significant inhibitory effect ( p < 0.01) at 3.89 μg mL −1 (8.68 μM), with 12.4% of inhibition. Our data corroborate previous findings on the inhibitory effects of both flavonols against 5-LOX [34,35]. In fact, not only the main components 1 and 7 have been previously reported as 5-LOX inhibitors, but also other flavonol derivatives identified on the hydroethanolic extract obtained from the leaves of S. senegalensis (Table 1 and Figure 2).…”

Section: Resultssupporting

confidence: 92%

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response

et al. 2019

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Predominantly spread in West Tropical Africa, the shrub Salacia senegalensis (Lam.) DC. is known because of its medicinal properties, the leaves being used in the treatment of skin diseases. Prompted by the ethnomedicinal use, a hydroethanolic extract obtained from the leaves of the plant was screened against a panel of microbial strains, the majority of which involved in superficial infections. The extract was found to be active against the dermatophytes Trichophyton rubrum and Epidermophyton floccosum. Notable results were also recorded regarding the attenuation of the inflammatory response, namely the inhibitory effects observed against soybean 5-lipoxygenase (IC50 = 71.14 μg mL−1), no interference being recorded in the cellular viability of RAW 264.7 macrophages and NO levels. Relevantly, the extract did not lead to detrimental effects against the keratinocyte cell line HaCaT, at concentrations displaying antidermatophytic and anti-inflammatory effects. Flavonoid profiling of S. senegalensis leaves was achieved for the first time, allowing the identification and quantitation of myricitrin, three 3-O-substituted quercetin derivatives, and three other flavonoid derivatives, which may contribute, at least partially, to the observed antidermatophytic and anti-inflammatory effects. In the current study, the plant S. senegalensis is assessed concerning its antidermatophytic and anti-inflammatory properties.

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Section: Resultssupporting

confidence: 92%

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response

et al. 2019

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“…The isolation of the main components as well as compounds that were present uniquely in C. spinosum was achieved by fractionation of the extract using countercurrent chromatography (CCC) in step-gradient elution mode. Gradient elution was chosen as an established and most effective method that has been successfully applied for the direct separation of compounds with a wide range of polarities from complex plant extracts [28][29][30]. In order to select a biphasic solvent system for efficient separation, we studied the partition behavior of the main components of the extract (cichoric and caftaric acid) in two simple binary systems: one of medium polarity composed of ethyl acetate: water in a ratio of 1 : 1 (v/v) and a second one of high polarity composed of n-butanol : water 1 : 1 (v/v).…”

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confidence: 99%

Phytochemical Analysis and Antioxidant Potential of the Phytonutrient-Rich Decoction of Cichorium spinosum and C. intybus

et al. 2016

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The Cretan diet, as the basis of the Mediterranean diet, has provided traditional remedies for the general well being of people through the long-established consumption of cooked wild greens and vegetables. The intake of the water decoctions of Cichorium spinosum and Cichorium intybus in the context of the daily dietary regime in Greece has been long associated with "liver detoxifying" properties. In the current study, we performed an in-depth investigation of the water decoctions traditionally prepared from C. spinosum and C. intybus through qualitative UHPLC-HRMS profiling and direct quantification of cichoric and caftaric acid as major antioxidant components of the decoction. In addition, we developed a one-step countercurrent chromatography method for the isolation of the two phenolic acids, along with a sulfoconjugate sesquiterpene lactone present only in the Cretan C. spinosum. All water decoctions were found not to be cytotoxic in human fibroblasts, whereas they all significantly reduced the intracellular reactive oxygen species, which is consistent with the major presence of strong antioxidant compounds such as cichoric acid. This work demonstrates that the intake of these decoctions in doses suggested by Greek traditional use is comparable to the ingestion of a phytomedical preparation of antioxidants. These results contribute to our current knowledge on the beneficial health effect of the Cretan diet.

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“…[ 18 ] In previous studies was found that compounds such as pinitol,[ 19 ] coumarin[ 20 ] myricitrin (a flavonoid glycoside) have been reported to have anti-inflammatory effects. [ 21 ] These substances found as secondary metabolites may be involved in anti-inflammatory mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory potential of Petiveria alliacea on activated RAW264.7 murine macrophages

Gutierrez

Hoyo‐Vadillo

2017

Phcog Mag

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Background:Defense and protection to multiple harmful stimuli are the inflammation, when is self-amplified and uncontrolled is the basis of the pathogenesis of a wide variety of inflammatory illness. The aim of this study was to evaluate if Petiveria alliacea could attenuate inflammation in a murine model of RAW264 macrophages the involved model and its involved mechanism.Materials and Methods:The ethanol extract from P. alliacea was precipitated with water and supernatant was used for this study (PW). The anti-inflammatory effects of PW were investigated through evaluating of the production of several cytokines, chemokines, and expression of nuclear factor-kappa B (NF-κB) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Also was determined the ability to decrease the oxidative stress in RAW264.7 cells with carboxy-2’,7’-dichloro-dihydro-fluorescein diacetate.Results:PW significantly suppress the secretion of prostaglandin E2, leukotriene C4, interleukin (IL)-1 β, IL-6, IL-10, interferon gamma nitric oxide (NO), inducible NO synthase, IL-1 β, IL-4, in RAW264.7 cells in a dose-dependent manner. In addition, PW also markedly inhibited the transcriptional activity of NF-κB. PW produced significant anti-inflammatory activity through inhibiting the production of inflammatory mediators through the NF-κB inactivation in the LPS-stimulated RAW24.7 cells.Conclusions:PW exerts significant antioxidant and anti-inflammatory activities, and this effect can be attributed in part, to the presence of dibenzyl disulfide, dibenzyl trisulfide pinitol, coumarin, myricetin, glutamyl-S-benzyl cysteine, and petiveriins A and B.SUMMARY Treatment with ethanol extract from Petiveria alliacea which was previously precipitated with water and supernatant (PE) was tested in LPS-stimulated RAW264.7 cells. PE suppressed the level of oxidative stress and the induction of proinflammatory mediators, as PGE2, LTC4, IL-1 ß, IL-6, IL-10, IFN- NO, iNOS, IL-1 ß, IL-4, in RAW264.7 macrophages through NF-B inactivation. These findings suggest that P. alliacea affords promising therapeutic in inflammatory diseases. Abbreviation used: COX-2: Ciclooxigenasa 2; DCFHDA: Carboxy-2’,7’-dichloro-dihydro-fluorescein diacetate; DMEM: Dulbecco's modified eagle's medium; FBS: Fetal bovine serum; HSP70: Heat shock protein; IFN-γ: Interferon gamma; IL-1 β: Interleukin 1 β, IL-6: Interleukin 6; IL-10: Interleukin 10; IL-4: Interleukin 4; iNOS: Nitric oxide synthase; KCl: Potassium chloride; LPS: Lipopolysaccharides; LTC4: leukotriene C 4; MgCl2: Magnesium chloride; MTT: 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide; NF-κB: Nuclear factor kappa-light-chain-enhancer of activated B-cells or transcriptional activity of nuclear factor-kB; NO: Nitric oxide; PBS: Phosphate-buffered saline; PGE2: Prostaglandin E2, PMSF: Phenylmethylsulfonyl fluoride; PTC: Chloroform extract from Petiveria alliacea; PE: Ethanol extract from Petiveria alliacea; PTH: Hexane extract from Petiveria alliacea; PW: Supernatant of PTE precipitated with water; RAW264.7: Cell...

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Phytochemical Profile of the Aerial Parts of Sedum sediforme and Anti-inflammatory Activity of Myricitrin

Cited by 8 publications

References 34 publications

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response

Flavonoid Composition of Salacia senegalensis (Lam.) DC. Leaves, Evaluation of Antidermatophytic Effects, and Potential Amelioration of the Associated Inflammatory Response

Phytochemical Analysis and Antioxidant Potential of the Phytonutrient-Rich Decoction of Cichorium spinosum and C. intybus

Anti-inflammatory potential of Petiveria alliacea on activated RAW264.7 murine macrophages

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