Phytochemical Profile, Plant Precursors and Some Properties of Georgian Propolis

Okińczyc, Piotr; Widelski, Jarosław; Ciochoń, Monika; Paluch, Emil; Bozhadze, Anna; Jokhadze, M; Mtvarelishvili, Gocha; Korona-Głowniak, Izabela; Krzyżanowska, Barbara; Kuś, Piotr Marek

doi:10.3390/molecules27227714

Cited by 5 publications

(20 citation statements)

References 43 publications

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“…In summary, this result suggests that a stronger presence of aspen markers (Mestia, Norio, Passanauri, Aspindza) and the dominance of non-flavonoid polyphenols over flavonoid in poplar-origin propolis (Qvakhreli) is connected with lower anti- H.pylori activity. Similar results were presented in previous research for Gram-positive bacteria [ 31 , 40 ]. Moreover, when hierarchical fuzzy clustering was used, almost the same results as PCA were obtained ( Figure 4 ).…”

Section: Resultssupporting

confidence: 92%

“…Composition of propolis extracts was analyzed in detail in our previous research [ 40 ]. In this study, the concentration of components was calculated as percentage of the whole UV chromatogram area (280 nm).…”

Section: Resultsmentioning

confidence: 99%

“…Results of matrix correlations are presented in Supplementary Table S1 . Total phenolic content (TP) and flavonoid content (TF) were described in previous research [ 40 ]. Generally, the activity against H. pylori was negatively correlated with TF and TP of extracts.…”

Section: Resultsmentioning

confidence: 99%

“…UHPLC-DAD-MS/MS analysis was performed according to previously developed method [ 40 ]. uHPLC-DAD-MS/MS was carried out using a compact QqTOF MS/MS detector (Bruker, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Antiradical activity (DPPH test) and total antioxidant activity (FRAP assay) and total phenolic content (TP) and total flavonoid content (FC) assays were performed according to previously described methods [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

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Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori

et al. 2023

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Helicobacter pylori (H. pylori) is considered the most common bacterial pathogen colonizing stomach mucosa of almost half the world’s population and is associated with various gastrointestinal diseases (from digestive problems and ulcers to gastric cancer). A lack of new drugs and a growing number of H. pylori antibiotic-resistant strains is a serious therapeutic problem.As a mixture of natural compounds, propolis has antimicrobial activity based on high concentrations of bioactive polyphenols (mainly flavonoids and phenolic acid derivates). The chemical composition of tested Georgian propolis is characterized by the presence of flavonoids aglycones, and phenolic acid monoesters, e.g., pinobanksin-5-methyl ether, pinobanksin, chrysin, pinocembrin, galangin, pinobanksin-3-O-acetate, pinostrobin and pinobanksin-3-O-butanoate, or isobutanoate and methoxycinnamic acid cinnamyl ester. The anti-H. pylori activity of 70% ethanol water extracts of 10 Georgian propolis samples was evaluated in vitro by MIC (minimal inhibitory concentration) against the reference strain (H. pylori ATCC 43504) and 10 clinical strains with different antibiotic-resistance patterns. The strongest anti-Helicobacter activity (MIC and MBC = 31.3 µg/mL) was observed for propolis from Orgora, Ota, and Vardzia and two from Khaheti. Lower levels of activity (MIC = 62.5 µg/mL) were found in propolis obtained from Qvakhreli and Pasanauri, while the lowest effect was observed for Norio and Mestia (MIC = 125.0 µg/mL). However, despite differences in MIC, all evaluated samples exhibited bactericidal activity. We selected the most active propolis samples for assessment of urease inhibition property. Enzyme activity was inhibited by propolis extracts, with IC50 ranging from 4.01 to 1484.8 µg/mL. Principal component analysis (PCA) and hierarchical fuzzy clustering (dendrograms) coupled with matrix correlation analysis exhibited that the strongest anti-Helicobacter activity was connected with black poplar origin and high flavonoid content of propolis. Samples with lower activity contained higher presence of aspen markers and/or dominance of non-flavonoid polyphenols over flavonoids. In summary, Georgian propolis can be regarded as a source bioactive compounds that can be used as adjuvant in therapy of H. pylori infection.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…UHPLC-DAD-MS/MS analysis was performed according to previously developed method [ 40 ]. uHPLC-DAD-MS/MS was carried out using a compact QqTOF MS/MS detector (Bruker, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori

et al. 2023

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Deciphering the potential of Cymbopogon citratus (DC.) Stapf as an anti-obesity agent: phytochemical profiling, in vivo evaluations and molecular docking studies

Aly,

Mekky,

Pereira

et al. 2024

Food Funct.

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Biochemical Profile and Antioxidant Properties of Propolis from Northern Spain

Rendueles,

Mauriz,

Sanz-Gómez

et al. 2023

Foods

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The antioxidant, anti-inflammatory, and antimicrobial characteristics of propolis, a bioactive compound collected from hives, have prompted its use in the food sector in recent times. This study investigated the physicochemical characteristics, phenolic profile, and antioxidant capacity of 31 propolis extracts collected from Northern Spain. The physicochemical composition (resins, waxes, ashes mineral content, and heavy metals) was within the allowable regulatory limits. The analysis of bioactive compounds enabled the identification of 51 constituents: flavonoids (apigenin, catechin, chrysin, quercetin, and pinocembrin) and phenolic acids (caffeic, ferulic, and coumaric). The mean value of total polyphenols was 42.72 ± 13.19 Pinocembrin–Galangin Equivalents/100 g, whereas a range between 1.64 ± 0.04 and 4.95 ± 0.36 Quercetin Equivalents (QE) g/100 g was found for total flavonoids content. The determination of bioactivities revealed significant antioxidant capacity using DPPH (1114.28 ± 10.39 µM Trolox Equivalents and 3487.61 ± 318.66 µM Vitamin C Equivalents). Resin content in propolis samples was positively and significantly correlated with both polyphenols (rho = 0.365; p = 0.043) and flavonoid composition (rho = 0.615; p = 0.000) as well as the antioxidant capacity TEAC DPPH (rho = 0.415; p = 0.020). A multiple regression analysis modeled the correlation between resin composition, flavonoids, and TEAC DPPH values, yielding a significant regression equation (R2 = 0.618; F (2,28) = 22.629; p < 0.000; d = 2.299). Therefore, evaluating physicochemical parameters and biological activities provides a promising framework for predicting propolis’ quality and antioxidant properties, thus suggesting its potential as a functional and bioactive compound for the food industry.

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Phytochemical Profile, Plant Precursors and Some Properties of Georgian Propolis

Cited by 5 publications

References 43 publications

Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori

Correlation between Chemical Profile of Georgian Propolis Extracts and Their Activity against Helicobacter pylori

Deciphering the potential of Cymbopogon citratus (DC.) Stapf as an anti-obesity agent: phytochemical profiling, in vivo evaluations and molecular docking studies

Biochemical Profile and Antioxidant Properties of Propolis from Northern Spain

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