Phytochemical profiling and mechanistic evaluation of black garlic extract on multiple sclerosis rat model

Elkhawas, Yasmin A.; Seleem, Mae; Shabayek, Marwa I.; Majrashi, Taghreed A.; Al-Warhi, Tarfah; Eldehna, Wagdy M.; Mostafa, Nada M.

doi:10.1016/j.jff.2023.105900

Cited by 2 publications

(3 citation statements)

References 68 publications

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“…Mass spectra were detected in the ESI negative and positive ion modes. Using the molecular weight, the fragmentation pattern of the mass spectrum, and comparison with previously published data, compounds were tentatively identified …”

Section: Methodsmentioning

confidence: 99%

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LC-ESI-MS/MS-Based Comparative Metabolomic Study, Antioxidant and Antidiabetic Activities of Three Lobelia Species: Molecular Modeling and ADMET Study

Elkhawas,

Gad,

AbdelRazek

et al. 2024

ACS Omega

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The hydroethanol (70%) extracts of three Lobelia species (L. nicotianifolia, L. sessilifolia, and L. chinensis) were analyzed using LC-ESI-MS/MS. Forty-five metabolites were identified, including different flavonoids, coumarin, polyacetylenes, and alkaloids, which were the most abundant class. By applying Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) based on LC-ESI-MS/MS analysis, the three species were completely segregated from each other. In addition, the three Lobelia extracts were tested for their antioxidant activities using a DPPH assay and as antidiabetic agents against αglycosidase and α-amylase enzymes. L. chinensis extract demonstrated significant antioxidant activity with an IC50 value of 1.111 mg/mL, while L. nicotianifolia showed mild suppressing activity on the α-glycosidase activity with an IC50 value of 270.8 μg/mL. A molecular simulation study was performed on the main compounds to predict their potential antidiabetic activity and pharmacokinetic properties. The molecular docking results confirmed the αglycosidase inhibitory activity of the tested compounds, as seen in their binding mode to the key amino acid residues at the binding site compared to that of the standard drug acarbose. Furthermore, the predictive ADMET results revealed good pharmacokinetic properties of almost all of the tested compounds. The biological evaluation results demonstrated the promising activity of the tested compounds, aligned with the in silico results.

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Section: Methodsmentioning

confidence: 99%

“…Using the molecular weight, the fragmentation pattern of the mass spectrum, and comparison with previously published data, compounds were tentatively identified. 44 Chemometric Analysis. The obtained information from LC-MS was subjected to chemometric analysis.…”

Section: ■ Conclusionmentioning

confidence: 99%

LC-ESI-MS/MS-Based Comparative Metabolomic Study, Antioxidant and Antidiabetic Activities of Three Lobelia Species: Molecular Modeling and ADMET Study

Elkhawas,

Gad,

AbdelRazek

et al. 2024

ACS Omega

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“…Black garlic has no pungent smell peculiar to garlic and has a sweet taste like dried fruit. Furthermore, black garlic has been reported to exhibit a number of bioactivities, including antioxidant, anti-inflammatory, and anti-cancer effects [5][6][7]. Sulfides, cysteine derivatives, allicin, and other sulfur-containing compounds have been proposed as key factors in garlic's antioxidant and other bioactivities [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies of Bioactivities and Chemical Components in Fresh and Black Garlics

Matsuse,

Hirata,

Abdelrahman

et al. 2024

Molecules

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To investigate the bioactivities of fresh garlic and its processed product, black garlic, we conducted comparative analyses of antioxidant, anti-inflammatory, innate immune activation, and anti-cancer activities in addition to the chemical composition (sugar, amino acid, and polyphenol contents) of these materials. Simultaneous assay using neutrophil-like cells showed that fresh garlic exhibited antioxidant and innate immunostimulatory activities, whereas black garlic displayed a potent anti-inflammatory effect. The antioxidant activity index was correlated with phenol and flavonoid contents, while the innate immunostimulatory activity was correlated with fructan content. Furthermore, some black garlics with low fructose content were found to inhibit the proliferation of UM-UC-3 cancer cells, while other black garlics rich in fructose increased UM-UC-3 cell proliferation. It was shown that the processing of fresh garlic could change the composition of sugars, antioxidants, and amino acids, which have different effects on neutrophil-like cells and UM-UC-3 cells, as well as on bioactivities.

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Phytochemical profiling and mechanistic evaluation of black garlic extract on multiple sclerosis rat model

Cited by 2 publications

References 68 publications

LC-ESI-MS/MS-Based Comparative Metabolomic Study, Antioxidant and Antidiabetic Activities of Three Lobelia Species: Molecular Modeling and ADMET Study

LC-ESI-MS/MS-Based Comparative Metabolomic Study, Antioxidant and Antidiabetic Activities of Three Lobelia Species: Molecular Modeling and ADMET Study

Comparative Studies of Bioactivities and Chemical Components in Fresh and Black Garlics

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