Phytochemical Properties, Antioxidant Activity and α- Amilase Inhibitory of Curcuma Caesia

Kartini, Sri; Juariah, Siti; Mardhiyani, Dini; Bakar, Moh Fadzelly Abu; Bakar, Fazleen Izzani Abu; Endrini, Susi

doi:10.37934/araset.30.1.255263

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…α-Amylase reduces the elevated level of glucose in the blood after eating by reducing the rate at which starch is converted to sugar. α-Glucosidase inhibitory potential was also increased in the extract, which shows that the plant has the potential to combat hyperglycemia ( Kartini et al, 2023 ). They prevent the digestion of carbohydrates, and they are competitive inhibitors.…”

Section: Discussionmentioning

confidence: 99%

GC-MS chemical profiling, antioxidant, anti-diabetic, and anti-inflammatory activities of ethyl acetate fraction of Spilanthes filicaulis (Schumach. and Thonn.) C.D. Adams leaves: experimental and computational studies

Ojo,

Ogunlakin,

Gyebi

et al. 2023

Front. Pharmacol.

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Introduction: This study aimed to investigate the chemical profile of GC-MS, antioxidant, anti-diabetic, and anti-inflammatory activities of the ethyl acetate fraction of Spilanthes filicaulis leaves (EFSFL) via experimental and computational studies.Methods: After inducing oxidative damage with FeSO4, we treated the tissues with different concentrations of EFSFL. An in-vitro analysis of EFSFL was carried out to determine its potential for antioxidant, anti-diabetic, and anti-inflammatory activities. We also measured the levels of CAT, SOD, GSH, and MDA.Results and discussion: EFSFL exhibited anti-inflammatory properties through membrane stabilizing properties (IC50 = 572.79 μg/ml), proteinase inhibition (IC50 = 319.90 μg/ml), and inhibition of protein denaturation (IC50 = 409.88 μg/ml). Furthermore, EFSFL inhibited α-amylase (IC50 = 169.77 μg/ml), α-glucosidase (IC50 = 293.12 μg/ml) and DPP-IV (IC50 = 380.94 μg/ml) activities, respectively. Our results indicated that induction of tissue damage reduced the levels of GSH, SOD, and CAT activities, and increased MDA levels. However, EFSFL treatment restores these levels to near normal. GC-MS profiling shows that EFSFL contains 13 compounds, with piperine being the most abundant. In silico interaction of the phytoconstituents using molecular and ensembled-based docking revealed strong binding tendencies of two hit compounds to DPP IV (alpha-caryophyllene and piperine with a binding affinity of −7.8 and −7.8 Kcal/mol), α-glucosidase (alpha-caryophyllene and piperine with a binding affinity of −9.6 and −8.9 Kcal/mol), and to α-amylase (piperine and Benzocycloheptano[2,3,4-I,j]isoquinoline, 4,5,6,6a-tetrahydro-1,9-dihydroxy-2,10-dimethoxy-5-methyl with a binding affinity of −7.8 and −7.9 Kcal/mol), respectively. These compounds also presented druggable properties with favorable ADMET. Conclusively, the antioxidant, antidiabetic, and anti-inflammatory activities of EFSFL could be due to the presence of secondary metabolites.

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Section: Discussionmentioning

confidence: 99%

GC-MS chemical profiling, antioxidant, anti-diabetic, and anti-inflammatory activities of ethyl acetate fraction of Spilanthes filicaulis (Schumach. and Thonn.) C.D. Adams leaves: experimental and computational studies

Ojo,

Ogunlakin,

Gyebi

et al. 2023

Front. Pharmacol.

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“…Phenolic compounds are a diverse group of secondary metabolites found in plants. They are known to have various beneficial effects on human health due to their antioxidant and antiinflammatory properties [21]. The total phenolic content (TPC) values for all harumanis leaves extracts were quantified using UV spectroscopy with gallic acid as the test standard.…”

Section: Total Phenolic and Flavonoid Contents Of Harumanis Leaves Ex...mentioning

confidence: 99%

Insights into the Pharmacognostic Elucidation of Harumanis Mango (Mangifera Indica Linn.) Leaves Extracts as Therapeutic Agent

Ryan Vitthaya Peron,

Amirul Ridzuan Abu Bakar,

Mohd Asraf Mohd Zainuddin

et al. 2024

ARMNE

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Mangifera indica L. (mango) of the Anacardiaceae family is a fruit with many phytochemicals that are extensively used in various traditional systems of medicine to prevent and treat various diseases. In Malaysia, harumanis is a local variety of mango widely cultivated in the northern region, namely Perlis. However, the drawback of this harumanis variety of mango is that research and information on its therapeutics are scarce and limited. Therefore, localized harumanis leaves were introduced to a series of pharmacognostic studies to provide critical details explaining harumanis as a potential therapeutic agent. Initially, the harumanis leaves were introduced to quality analysis tests, unveiling that the raw material used abided by proper post-harvesting protocols. Then, the harumanis leaves were extracted using ultrasonic aided extraction (UAE) with 100% methanol (13.73±0.24 %), 50% methanol (14.47±0.19 %), 100% ethanol (10.35±0.19 %), 50% ethanol (12.61±0.26 %), and distilled water (8.09±0.21 %). Next, the initial phytochemical screening revealed the presence of flavonoids, tannins, and saponins in all extracts. Subsequently, the phenolic and flavonoid content were quantified with the 50% methanol extracts recorded the highest total phenolic content (5.76 ± 0.02 mg/g), 50% ethanol extracts had the highest total flavonoid content (4.38 ± 0.04 mg/g). Fingerprinting analysis via FTIR spectroscopy was used to identify phytochemicals confirming the presence of flavonoids and phenolics compounds in all extracts. The extracts were then introduced to a DPPH inhibition assay with the highest IC50 value obtained from 50% methanol with 75.2 ± 0.22 microgram per milliliter. Next, the antimicrobial capabilities were tested using a disc diffusion study on E. coli, B. subtilis, and Face normal flora showing that the methanolic extract has superior abilities in microbial inhibitions with the value recorded at 13mm, 22mm, and 14mm respectively. Overall, we obtained valuable pharmacognostic data on the harumanis leaves extracts as potential antioxidant and antimicrobial agents.

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“…Sample extract of four different concentration were analysed to identify the impact of the solvent type and the extract concentration on the antioxidant activity of the coconut waste. The evaluation was conducted by the DPPH method, employing a spectrophotometer, which operates on the principle that the purple colour of DPPH is reduced to light yellow as a result of the donation of hydrogen atoms by the antioxidants, thereby forming stable DPPH radicals [27]. Results in Figure 3 showed that, the radical scavenging activity of coconut shell and husk were concentration dependent.…”

Section: Antioxidant Activity Of Coconut Wastementioning

confidence: 99%

Optimization of Extraction Solvents on the Antioxidant Properties of Coconut Waste

Husna Filzah Ismail,

Fazrena Nadia Md Akhir,

Nor’azizi Othman

et al. 2024

ARFMTS

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Cocos nucifera L. (family Arecaceae) commonly known as coconut is considered as an important fruit crop in tropical countries and are widely used for therapeutic and domestic purpose. They have effective properties such as antioxidant, antitumor, antiseptic and antimicrobial. The growing demand for green coconut water consumption and food industries cause the dumping of the shell and husk of this fruit, generating large amount of solid waste. This study utilized part of coconut waste to study the impacts of solvent type on the phenolic content. Six different polarities of solvent were chosen. Total phenolic content was performed using Folin-Ciocalteu assay and free radical scavenging activity with 2,2-diphenyl-1-picrilhydrazil (DPPH) method. Total phenolic content for coconut shell was the highest at 71.57 ± 0.275 mg GAE/g for propanol extract and 74.10 ± 0.741 mg GAE/g for acetone extract of coconut husk. The antioxidant activity of all sample extracts was analysed using DPPH assay. Highest radical scavenging activity of coconut husk was observed by propanol extract with 93.82 ± 0.052 % while the lowest scavenging activity was demonstrated by chloroform extract with percentage of 57.77 ± 2.255%. Acetone extract of the shell exhibited the highest scavenging activity of 91.23 ± 0.073%, while chloroform extract of coconut shell demonstrated the lowest antioxidant activity of 70.627 ± 0.467%.

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Phytochemical Properties, Antioxidant Activity and α- Amilase Inhibitory of Curcuma Caesia

Cited by 6 publications

References 23 publications

GC-MS chemical profiling, antioxidant, anti-diabetic, and anti-inflammatory activities of ethyl acetate fraction of Spilanthes filicaulis (Schumach. and Thonn.) C.D. Adams leaves: experimental and computational studies

GC-MS chemical profiling, antioxidant, anti-diabetic, and anti-inflammatory activities of ethyl acetate fraction of Spilanthes filicaulis (Schumach. and Thonn.) C.D. Adams leaves: experimental and computational studies

Insights into the Pharmacognostic Elucidation of Harumanis Mango (Mangifera Indica Linn.) Leaves Extracts as Therapeutic Agent

Optimization of Extraction Solvents on the Antioxidant Properties of Coconut Waste

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