Phytochemical response of Stevia plant to growth promoting microorganisms under salinity stress

Forouzi, Arezou; Ghasemnezhad, Azim; Nasrabad, Reza Ghorbani

doi:10.1016/j.sajb.2020.04.001

Cited by 23 publications

(13 citation statements)

References 69 publications

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“…To estimate total phenol contents, the procedure of Forouzi et al, 2020 and Ahmad et al, 2001 was followed [92,93]. Briefly, leaf alcoholic extract (20 µL) was mixed with deionized water (1.15 mL) and folin ciocalteu reagent (0.1 mL).…”

Section: Total Flavonoids and Phenol Estimationmentioning

confidence: 99%

Biochemical Characterization of Halotolerant Bacillus safensis PM22 and Its Potential to Enhance Growth of Maize under Salinity Stress

Azeem

Shah

Ullah

et al. 2022

Plants

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Salinity stress is one of the primary abiotic stresses limiting crop growth and yield. Plants respond to salinity stress with several morphophysiological, molecular, and biochemical mechanisms, however, these mechanisms need to be improved further to cope with salt stress effectively. In this regard, the use of plant growth-promoting (PGP) and halotolerant bacteria is thought to be very efficient for enhancing growth and salinity tolerance in plants. The current study aims to assess Bacillus safensis PM22 for its ability to promote plant growth and resistance to salt. The PM22 produced substantial amounts of exopolysaccharides, indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylic acid deaminase (ACC-deaminase) under saline conditions. Additionally, inoculation of the halotolerant bacteria PM22 reduced the severity of salinity stress in plants and increased root and shoot length at various salt concentrations (0, 180, 240, and 300 mM). Furthermore, PM22-inoculated plants showed markedly enhanced photosynthetic pigment, carotenoid, leaf relative water content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity, salt tolerance index, total soluble sugar, total protein, and ascorbic acid contents compared to non-inoculated control maize plants. PM22 substantially increased antioxidant (enzymatic and non-enzymatic) activities in maize plants, including ascorbate peroxidase, peroxidase, superoxide dismutase, catalase, total flavonoid, and phenol levels. Maize plants inoculated with PM22 also exhibited a significant reduction in electrolyte leakage, hydrogen peroxide, malondialdehyde, glycine betaine, and proline contents compared to non-inoculated control plants. These physiological appearances were further validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), which revealed the upregulation of expression in genes responsible for stress tolerance. In the current investigation, Bacillus safensis PM22 showed plant growth-promoting and salt tolerance attributes and can be utilized as a bio-inoculant to improve yield in salt stress affected areas.

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Section: Total Flavonoids and Phenol Estimationmentioning

confidence: 99%

Biochemical Characterization of Halotolerant Bacillus safensis PM22 and Its Potential to Enhance Growth of Maize under Salinity Stress

Azeem

Shah

Ullah

et al. 2022

Plants

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“…However, irrigation with saline water might cause its growth retardation and affect negatively its production (Kurunc et al 2020). According to many authors (Shahverdi et al, 2017(Shahverdi et al, , 2018Yavaş et al 2019;Forouzi et al 2020), salt stress has harmful effects on plant growth, photosynthetic apparatus, ion content, and antioxidant enzymes activity. Based on all the given information, the aim of this study was to evaluate the potential roles of Rhizophagus irregularis (Ri) and arbuscular mycorrhizal consortium (MC) treatment types on physiological and biochemical behaviors of S. rebaudiana subjected to salt stress.…”

Section: Introductionmentioning

confidence: 99%

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Janah

Meddich

Elhasnaoui

et al. 2021

J Soil Sci Plant Nutr

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Salinization of soil is a major problem affecting many plant physiological processes. The use of arbuscular mycorrhizal fungi (AMF) plays a key role in improving the growth and the crops yield. The aim of this study was to evaluate the combined effects of salt stress and AMF on agro-physiological and biochemical responses of Stevia rebaudiana Bertoni. The experiment was carried out in a completely randomized design with ten replicates under the green house conditions. Stevia plants were inoculated with AMF consortium (MC) or with Rhizophagus irregularis (Ri) on the presence (80 mM of NaCl) or the absence (0 mM of NaCl) of salt stress. The results showed that plant height, dry biomass of shoots and roots parts, mycorrhizal frequency and intensity, relative water content, chlorophyll fluorescence, stomatal conductance, chlorophyll a, and total chlorophyll were significantly reduced by 29%, 72%, 36%, 84%, 65%, 24%, 17%, 60%, 28.50%, and 40.9% respectively under salt stress. These changes were associated with a significant increase in antioxidant enzymes (activity of polyphenol oxidase, peroxidase, superoxide dismutase, and catalase), electrolyte leakage, lipid peroxidation, and hydrogen peroxide contents. The application of AMF (MC and Ri) improved growth and physiological parameters through rising the activities of antioxidant enzymes and reduction of the oxidative damage caused by the salt stress. The better results were recorded with MC-inoculated plants. These findings suggested that AMF could be an effective strategy to alleviate the adverse effects of salt stress on Stevia rebaudiana.

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“…When comparing the antioxidant efficacy of stevia leaf extract treated with fungus against Streptomyces isolates, it seems that plants treated with fungi have greater potency. A higher inhibitory activity can be attributed to phenylpropanoid content [ 82 ]. Alleviation of salt stress in G. glabra by arbuscular mycorrhiza is also reported previously.…”

Section: Discussionmentioning

confidence: 99%

Microbial amelioration of salinity stress in endangered accessions of Iranian licorice (Glycyrrhiza glabra L.)

et al. 2022

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Background Glycyrrhiza glabra L. is a medicinal and industrial plant that has gone extinct due to different abiotic stress caused by climate change. To understand how the plant-associated microorganism can support this plant under salinity, we collected sixteen Iranian accessions of G. glabra L., inoculated their rhizomes with Azotobacter sp. (two levels, bacterial treatments, and no-bacterial treatments, and grown them under salinity stress (NaCl levels; 0, and 200 mM). Results Two accessions of Bardsir and Bajgah significantly showed higher resistant to salinity, for example by increasing crown diameter (11.05 and 11 cm, respectively) compared to an average diameter of 9.5 in other accessions. Azotobacter inoculation caused a significant increase in plant height and crown diameter. Among studied accessions, Kashmar (46.21%) and Ilam (44.95%) had the highest rate of membrane stability index (MSI). Evaluation of enzyme activity represented that bacterial application under salinity, increased polyphenol oxidase (PPO) (0.21 U mg−1 protein), peroxidase (POD) (3.09 U mg−1 protein U mg−1 protein), and phenylalanine ammonia-lyase (PAL) (17.85 U mg−1 protein) activity. Darab accession showed the highest increase (6.45%) in antioxidant potential compared with all studied accessions under Azotobacter inoculation. According to principal component analysis (PCA), it was found that the accession of Meshkinshahr showed a more remarkable ability to activate its enzymatic defense system under salt stress. Also, three accessions of Meshkinshahr, Eghlid, and Ilam were categorized in separated clusters than other accessions regarding various studied treatments. Conclusion Analysis indicated that five accessions of Meshkinshahr, Rabt, Piranshahr, Bardsir, and Kermanshah from the perspective of induced systematic resistance are the accessions that showed a greater morphophysiological and biochemical outcome under salinity. This study suggested that, inoculation of with Azotobacter on selected accession can relieve salt stress and support industrial mass production under abiotic condition.

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Phytochemical response of Stevia plant to growth promoting microorganisms under salinity stress

Cited by 23 publications

References 69 publications

Biochemical Characterization of Halotolerant Bacillus safensis PM22 and Its Potential to Enhance Growth of Maize under Salinity Stress

Biochemical Characterization of Halotolerant Bacillus safensis PM22 and Its Potential to Enhance Growth of Maize under Salinity Stress

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Microbial amelioration of salinity stress in endangered accessions of Iranian licorice (Glycyrrhiza glabra L.)

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