Phytochemical Screening and Antimicrobial Activity of Crude Stem Bark Extracts of Anogeissus leiocarpus

Alhassan, Dahiru; Uba, Abdullahi Ibrahim; Muhammad, Auwalu; Muhammad, Y

doi:10.9734/ejmp/2016/22443

Cited by 9 publications

(4 citation statements)

References 14 publications

(14 reference statements)

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“…The results reported in our study agree with the study reported previously where saponins and flavonoids were detected [27]. In a similar study, flavonoids were detected in the ESBE of A. leiocarpus while alkaloids were absent which agrees with the present study [28]. Previous studies on phytochemical components of stem bark extracts of A. leiocarpus reported results similar to our study [29,30].…”

Section: Resultssupporting

confidence: 93%

Phytochemical profiling and heavy metals composition of aqueous and ethanol extracts of Anogeissus leiocarpus

DAHIRU

Badgal

Musa

2022

Ankara Ecz. Fak. Derg.

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Objective: The present study aimed to investigate the phytochemical and heavy metals components of A. leiocarpus considering its applications in ethnomedicine. Material and Method: The phytochemical components were determined qualitatively and quantitatively gravimetrically, while component identification was done using (Gas spectrometer-mass spectrometer (GC-MS) technique. Heavy metals were quantified by atomic absorption spectrophotometer. Result and Discussion: Saponins and flavonoids were detected in the aqueous extracts in concentrations of 10.22% ±0.48, and 38.67% ±0.17 respectively, and concentrations of 17.37% ±0.65 and 19.63% ±0.60 respectively in the ethanol extract.. GC-MS analysis identified 16 and 26 compounds in the aqueous and ethanol extracts respectively. In the aqueous extract, 5-Hydroxymethylfurfural, 1,2,4-Benzenetriol, and cis-Vaccenic acid had the highest peak areas of 46.24, 17.12, and 15.13% respectively, while in the ethanol extract 5-Hydroxymethylfurfural (14.40%),1,2,3-Benzenetriol (12.29%) and -methoxybenzene-1,4-diol (7.54%) were the highest. Chromium (0.548 ppm ±0.030) was detected only in the aqueous concentration, while Cadmium had a concentration of 0.002 ±0.001 and 0.006 ppm ±0.002 in the aqueous and ethanol extract respectively. Lead was present with aqueous and ethanol extracts concentrations of 0.096 ±0.020 and 0.096 ppm ±0.040 respectively. Conclusively, the present study agrees with the claims for the traditional application of the plant in folkloric medicine to manage different ailments.

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Section: Resultssupporting

confidence: 93%

Phytochemical profiling and heavy metals composition of aqueous and ethanol extracts of Anogeissus leiocarpus

DAHIRU

Badgal

Musa

2022

Ankara Ecz. Fak. Derg.

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“…In the DPPH assay, the antioxidants are able to reduce the stable radical DPPH to nonradical form, DPPH-H. Various mechanisms have been put forward to explain the antioxidant activity of phenolic compounds including reducing capacity, prevention of chain initiation, binding of transition metal ion catalysts, decomposition of peroxides, prevention of continued hydrogen abstraction and radical scavenging (Vasanthi et al, 2014). The reducing capacity of a compound may serve as a significant indicator of its potential antioxidant activity (Chanda et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Studies on In-vitro Bioactivity of Icacina trichantha Methanol Seed Extract

Orimisan

Olakunle

Morenikeji

2023

IJSGS

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Plants are new sources of antioxidative and antimicrobial agents, hence the need to determine and evaluate the antibacterial, antifungal properties and antioxidant activity of medicinal plants. The aim of this study was to investigate antioxidant and antimicrobial potentials of methanol extract of Icacina trichantha. Oliv. (Icacinaceae) seed. In this research work, antioxidant study was done by using various in vitro methods such as: 1-Dipheny-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline)-6-sulfonic acid) (ABTS), total antioxidant capacity (TAC), nitric oxide (NO) scavenging, hydrogen peroxide (H2O2) scavenging, ferric reducing antioxidant power, superoxide radical scavenging and hydroxyl radical scavenging assays. Antimicrobial properties were determined using agar cup diffusion method on MRSA, P. aeruginosa, S. typhi, C. krusei, S. dysenteriae, S. pyrogenes, E. coli, K. pneumoniae, C. albicans, and C. tropicalis. The extract of the studied plant exhibited pronounced antioxidant activities in the various antioxidant activity assay tested. Also, the extract had remarkable susceptibility effect on the tested microorganism with the exception of P. aeruginosa, S. typhi and C. tropicalis that were resistant. The findings indicate that the extract was found to be effective free radical quencher, a potent source of natural antioxidants and antimicrobial agents, thus justifying its traditional use in green therapeutics.

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“…In other cases, the plant parts are ground to powder and applied to external wounds to prevent infection to allow the healing process to take place (23). In experimental studies, AL was reported to exhibit antihyperglycemic (24), antioxidant (24), antihyperlipidemic (24), and antimicrobial effects (25) through different modes of action attributed to its phytoconstituents including alkaloids, glycosides, and flavonoids. However, there are limited studies revealing the potential mechanisms of action…”

Section: (−)-mentioning

confidence: 99%

Phytochemical Analysis, In-vitro, and In-silico Antibacterial Activity of Stembark Extract of Anogeissus leiocarpus (DC) Guill and Perr

Dahiru,

Abaka,

Musa

2023

sciphar

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Bacterial infections subsequently leading to antibiotic resistance has been a leading cause of mortality and morbidity worldwide especially in developing countries with high poverty rate and poor healthcare system. Thus, prompting the prospect in alternative therapy such as medicinal plants. In the present study, we evaluated the antibacterial action of stem bark extract of Anogeissus leiocarpus (AL) Guill and Perr. as applied in folkloric medicine for antibacterial purposes. The phytochemicals present in the plant extract were identified and quantified, followed by the determination of the antibacterial effects of the extract against Escherichia coli and Staphylococcus aureus. Molecular docking study was carried out to ascertain the inhibitory effects of compounds from AL against bacterial enzymes. Alkaloids (7.17% ±0.60), saponins (11.33% ±3.18), and flavonoids (31.01% ±4.04) were detected. A maximum ZI was observed for E. coli compared to S. aureus at the highest extract concentration (100 mg/mL) with amoxicillin having superior ZI at 50 mg/mL concentration. The MIC against E. coli and S. aureus were 12.5 mg/mL and ≤ 6.25 mg/mL respectively while the MBC was>100 mg/mL and 100 mg/mL respectively. Among the identified compounds, IX exhibited the least binding affinity (BA) (7.2 kcal/mol) and inhibition constant (Ki) (5 µM) against UDP-N-acetylglucosamine Enolpyruvyl Transferase (Mur A) compared to all the other targets. AL demonstrated antibacterial activity evidenced by the bacterial growth inhibition, bactericidal potential, and in-silico study revealing high affinity of the bacterial enzymes for the identified compounds, thereby supporting the acclaimed antibacterial use of the plant in folkloric medicine.

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Phytochemical Screening and Antimicrobial Activity of Crude Stem Bark Extracts of Anogeissus leiocarpus

Cited by 9 publications

References 14 publications

Phytochemical profiling and heavy metals composition of aqueous and ethanol extracts of Anogeissus leiocarpus

Phytochemical profiling and heavy metals composition of aqueous and ethanol extracts of Anogeissus leiocarpus

Studies on In-vitro Bioactivity of Icacina trichantha Methanol Seed Extract

Phytochemical Analysis, In-vitro, and In-silico Antibacterial Activity of Stembark Extract of Anogeissus leiocarpus (DC) Guill and Perr

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