Phytochemical Screening and Antimicrobial Effects of Aqueous and Ethanol Leaf and Stem Extracts of Gongronema latifolium Benth.

Ilodibi, Chinyere V.; Ezeja, Ijeoma J.; Akachuk, Ebele E.; Chukwum, Maureen U.; Egboka, Tochukwu P.; Emeka, Adaeze N.

doi:10.3923/rjb.2015.50.60

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…Chlohexidine promotes wound healing through its antimicrobial properties [20], whereas natural extracts are enriched in bioactive compounds that facilitate healing through multiple effects. For example, studies showed that quince fruit extract is enriched in tannins and phenolic compounds that induce healing of wounds and inflamed mucous membranes during the inflammatory phase through chelation of free radicals, enhancing wound contraction, formation of new capillary vessels and fibroblasts and keratinocytes proliferation in addition to their antibacterial activity [21], [22], [23], [24]. Vol.18.Issue 2,June 2020…”

Section: Discussionmentioning

confidence: 99%

Determination of Antibacterial and Wound Healing Potentials of Ethanolic Extract of Cydonia oblonga Miller Fruit

Najeeb¹,

Abdulaziz²,

Kurtas³

2020

DJM

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Background: Cydonia oblonga Miller (Quince) is one of the oldest cultural plants that belongs to the Rosaceae family. The bioactive components of Quince fruit are thought to have antimicrobial properties and able to accelerate the process of wound healing. The active ingredients that have antibacterial activity such as polyphenols can overcome the resistant mechanisms of bacteria and enhance killing through disturbing their metabolic activities. Objective: To determination of antibacterial and wound healing potentials of ethanolic extract of Cydonia oblonga Miller Fruit. Patients and Methods: The extract was prepared using hydroalcoholic extraction of quince whole fruit. Predominant wound bacteria isolates were obtained from active wounds on oral cheek mucosa of Albino Rabbits in a pilot study in which swab samples were taken from the induced oral wounds on the 2 nd day and immediately sent to the lab. The specimens were cultured on bacteriological media under aerobic and CO 2 conditions at 37°C for 24hr. discrete colonies were identified using microscopy, cultural characteristics and biochemical examinations. Different concentrations of the extract (15, 30, 60, 90 and 120mg/ml) using well-diffusion agar method were tested for their antibacterial activity. In another pilot study, the effective extract concentration on wound healing was determined after 3, 5, 7 days post wounding. The extract concentration showed the best healing activity was used later and compared with normal healing (without treatment) and chlorhexidine-treated wounds. Histological assessment were used to judge on healing activity. Results: Twelve bacterial isolates belonging to Neisseria sp., Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were tested. On the whole, all the isolates showed sensitivity to quince extract especially at high extract concentrations. Quince extract 60mg/ml oral irrigation showed significant antibacterial effect following 3 days post wounding as no bacterial colonies were detected in the test group when compared with the negative and positive control groups with sever inflammatory infiltrate in all groups. Nearly, complete healing of the wounds after 5 and 7 days was recorded in all groups. Conclusion: Cydonia oblonga Miller fruit ethanolic extract could be regarded as a good antibacterial substance with broad spectrum activity that promotes wound healing as well. The best concentration for both was 60mg/ml.

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Section: Discussionmentioning

confidence: 99%

Determination of Antibacterial and Wound Healing Potentials of Ethanolic Extract of Cydonia oblonga Miller Fruit

Najeeb¹,

Abdulaziz²,

Kurtas³

2020

DJM

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“…Different substances have been identified in medicinal plants which are believed to be antimicrobial agents and these includes; different forms of alkaloids, diterpenes, saponins, flavonoids, tannins, sterols, phenols, different forms of other proteins as well as lipids [3]. Several plant species have been tested for phytochemical and anti-microbial properties but the vast majorities have not yet been adequately evaluated [1,[4][5][6]. Among the medicinal plants, locally used in our community for treatment and management of various ailments is V. chrysocarpa.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Antimicrobial Studies on Vitex Chrysocarpa (Planch ex Benth.)

Ilodibia

Okafor

Akachukwu

et al. 2016

AJLSR

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Ethanolic leaf, stem and root extracts of Vitex chrysocarpa were investigated for the presence and composition of these phytochemicals (alkaloid, flavonoid, phenol, tannin, sterol, hydrogen cyanide, anthraquinone, saponin and terpenoid) using standard techniques. Their antimicrobial activities at different concentration against some selected clinical pathogens (bacterial strains Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, Fungal strain Aspergilus niger) were also studied using agar diffusion method. Minimum Inhibitory Concentration against the organisms was also evaluated. Analysis of Variance (ANOVA) was employed in data analysis. Qualitative and percent quantitative phytochemical results showed that Ethanolic leaf, stem and root extracts contained these phytochemicals assayed but at varied quantities. Antimicrobial studies indicated that the ethanolic leaf, root and stem extracts of Vitex chrysocarpa inhibited the growth of the pathogens but at varied levels and the inhibition was extracts concentration dependent. However, the extracts showed higher inhibition against the fungal strain than the bacterial strains. Inhibitory effect of the leaf extract was significantly higher than those of the stem and root extracts with minimum inhibitory concentration of 50 mg/ml for S. aureus and E. coli, 25 mg/ml for P. aeruginosa and 12.50 mg/ml for A. niger. For the stem, the minimum inhibitory concentration is at 50 mg/ml for S. aureus and P. aeruginosa, 75 mg/ml for E. coli and 25 mg/ml for A. niger. For the root, minimum inhibitory concentration is at 75 mg/ml for S. aureus and E. coli, 25 mg/ml for P. aeruginosa and 50 mg/ml for A.niger. Antibiotic had a better activity when compared to the extracts at the same concentration. The data obtained from this study showed that the plant possessed antimicrobial properties especially antifungal and could be used in the treatment of bacterial and fungal infections but more especially the latter.

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“…These plants were chosen for this research because of so many reports on them to possess some medicinal properties Ebana et al (2016). andIlodibia et al (2015) reported that ethanol and aqueous leaf extracts of Gongronema latifolium have antimicrobial activities on bacterial organisms including Staphylococcus aureus and Escherichia coli. The veterinary importance of both infectious diseases includes: decreased weight gain in meat birds, drop in egg production in laying birds, mortality, cost of treatment, condemnation of carcass at slaughter, and economic loss(Landman & Van- Eck, 2015).…”

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confidence: 99%

In-vitro antimicrobial potential of aqueous and metanolic extracts of Piper guineense and Gongonema latifolium on Staphylococcu aureus and Escherichia coli

2022

JoSVAS

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Plants are rich in different secondary metabolites, such as tannins, terpenoids and alkaloids which have been found in- vitro to have antimicrobial properties. The aim of this study was to determine the in-vitro antimicrobial potential of the methanolic and aqueous extracts of dried leaves of Gongronema latifolium and Piper guineense plants on Staphylococcus aureus and Escherichia coli. Soxhlet extractor was used for the methanolic solvent while cold maceration was used for the aqueous extraction. The phytochemical constituents of these extracts were screened thereafter the extracts were tested for their antimicrobial activity. The technique of agar well diffusion was employed. Wells was made on nutrient agar media and the extracts were placed in the well. Concentration of 1.25mg/ml, 2.5mg/ml, 5mg/ml and 10mg/ml were used. Surface of agar is inoculated (using sterile swab dipped into standardized broth culture of the test organism). Thereafter, the wells were bored into the inoculated agar and the extracts delivered into the well. After allowing for diffusion, the plates were incubated, after which the zones of inhibition around the well measured with the aid of vernier caliper. Activity of the extract against the test organism was expressed in terms of size (mm) of inhibition zone diameter around the well. The presence of alkaloids, flavonoids, tannins, saponins, and phenol in both plant extracts was observed. The aqueous extract of P. guineense and G latifolium showed more presence of phytochemical constituents than the methanolic extract and this is evidenced by higher zones of inhibition. Methanolic extracts of Piper guineense showed visible clearance suggestive of antibacterial activities by the extract only at 10mg/ml on S. aureus, but no form of inhibition on E. coli. Both extracts have concentration dependent antimicrobial effect on Staphylococcus aureus and Escherichia coli, and this may be due to phytochemicals found in them

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Phytochemical Screening and Antimicrobial Effects of Aqueous and Ethanol Leaf and Stem Extracts of Gongronema latifolium Benth.

Cited by 5 publications

References 13 publications

Determination of Antibacterial and Wound Healing Potentials of Ethanolic Extract of Cydonia oblonga Miller Fruit

Determination of Antibacterial and Wound Healing Potentials of Ethanolic Extract of Cydonia oblonga Miller Fruit

Phytochemical and Antimicrobial Studies on Vitex Chrysocarpa (Planch ex Benth.)

In-vitro antimicrobial potential of aqueous and metanolic extracts of Piper guineense and Gongonema latifolium on Staphylococcu aureus and Escherichia coli

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