Phytochemical screening and evaluation of in vitro antioxidant and antimicrobial activities of the indigenous medicinal plant Albizia odoratissima

Abstract

Context: Albizia odoratissima (L. f.) Benth has been used in Indian folk medicine to treat numerous inflammatory pathologies, such as leprosy, ulcers, burns and asthma. Objective: To evaluate the antioxidant and antimicrobial properties of A. odoratissima. Materials and methods: Dried leaves of A. odoratissima were extracted in organic solvents (hexane, chloroform, ethyl acetate, and methanol). The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and ferric reducing antioxidant power (FRAP) assays. The antibacterial activity was examined using minimum inhibitory concentration (MIC) and the minimum bacterial concentration (MBC), determined by broth microdilution method against Gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Proteus vulgaris) and Gram-positive bacterium (Staphylococcus aureus). Results: The TPC ranged from 4.40 ± 1.06 to 1166.66 ± 31.85 mg GAE/g of dry weight (DW), and the TFC ranged from 48.35 ± 3.62 to 109.74 ± 1.84 mg QE/g of DW. The IC50 values of the ethyl acetate extract for DPPH, ABTS, and H2O2 were 10.96 ± 0.40, 4.35 ± 0.07, and 163.82 ± 1.52 μg/mL, respectively. Both methanol and ethyl acetate extracts demonstrated effective antibacterial activity with MICs and MBCs values ranging 136–546 μg/mL and 273–1093 μg/mL, respectively, against the tested pathogenic species. Conclusions: The leaves of A. odoratissima showed potent free radical scavenging property and antimicrobial activity.

Design and synthesis of oxaprozin‐1,3,4‐oxadiazole hybrids as potential anticancer and antibacterial agents

“…Additionally, the newly synthesized analogues 5a‐p were screened for their in vitro antibacterial activity (Table ) against Gram‐positive bacteria ( Staphylococcus aureus ) and Gram‐negative bacteria ( Klebsiella pneumoniae , Pseudomonas aeruginosa and Escherichia coli ) by Agar well diffusion method . As anticipated and from the bacterial investigations, it is obvious that all the tested derivatives showed enhanced bacterial inhibition than the oxaprozin core.…”

“…The bacterial stock cultures were maintained on Mueller Hinton Agar (MHA) slants and stored at 4°C. Agar‐well diffusion method was employed for evaluation of antibacterial activity. The bacterial strains were reactivated from stock cultures by transferring into Mueller Hinton Broth (MHB) and incubating at 37°C for 18 hours.…”

Synthesis and Biological Evaluation of New Ibuprofen‐1,3,4‐oxadiazole‐1,2,3‐triazole Hybrids

“…aeruginosa ), and Escherichia coli ( E . coli ) by Agar‐well diffusion method , and the screening results are presented in Table . Norfloxacin and DMSO were used as a standard reference and a negative control, respectively.…”

Effect of Juglansregia and Nasturtum officinalis on biochemical parameters following toxicity of kidney by CCl4 in Wistar rats

“…Phenolic and flavonoid compounds in plants, have potent antioxidant activity, due to they have a functional group with antioxidant and scavenging activities (16). The hepatotoxic effects of CCl4 are mostly caused by peroxidation of lipids and the presence of the free radicals (17). In the recent work, the hepatotoxicity was demonstrated by significant increases in the activity of AST, ALT and ALP enzyme markers in CCl4 treated animals compared to normal group.…”

“…The J. regia extract reduces systolic and diastolic blood pressure. It also increased plasma renin levels and significantly reduced the ratio of aldosterone to rennin (17). In another study, the effect of hydroalcoholic extract of N. officinalis leaves on the lipid profile in rats in diabetic rats has been investigated.…”