Phytochemical screening and in-vitro activities of dichloromethane leaf extract of leptadenia hastata (pers.) decne against pathogens

Umaru, Isaac John; Umaru, Hauwa A.

doi:10.15406/ijvv.2018.05.00099

Cited by 1 publication

(1 citation statement)

References 7 publications

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“…All the test organisms were susceptible to the extract, albeit at different concentrations and MICs (Table 5). Umaru and Umaru (2018) projected the antibacterial activities of various concentration of dichloromethane crude extract of the plant Leptadenia hastata that was tested against some pathogenic bacteria such as; K. pneumoniae, E. coli, S. aureus and Salmonella typhi. All the concentrations used were active against all test organisms, the highest activity was recorded against E. coli and S. aureus at the concentrations of 500 ppm and 1000 ppm (1.03±0.06 mm, 1.23±0.06 mm and 1.23±0.06 mm, 1.33±0.06 mm) respectively.…”

Section: Qualitative and Quantitative Phytochemical Analysismentioning

confidence: 99%

Comparative antibacterial efficacies of the extracts of <i>Enantia chlorantha</i> (Awopa) on selected clinical isolates

Kamoldeen,

Toyosi,

Yetunde

et al. 2023

SWJ

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Researchers and health scientists are challenged by the phenomenon of antimicrobial resistance which has necessitated the need for the discovery of novel antimicrobial agents. This study evaluated the antimicrobial efficacy of the bark of Enantia chlorantha (Awopa) based on extractions using selected polar and non-polar solvents (Aqueous, Dichloromethane, Petroleum ether, and N-hexane). Antimicrobial activities were investigated against six clinical isolates (Candida albicans, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Pseudomonas aeruginosa) via the agar well diffusion assay. Minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined by the macro broth dilution method. The phytochemical components of the extracts were analyzed qualitatively and quantitatively. The dichloromethane extract of E. chlorantha expressed the highest antimicrobial activity against all the test organisms with K. pneumonia being the most susceptible with (21.50 mm zone of inhibition) at a concentration of 50mg/mL; while C. albicans was the least susceptible organism with 3.00 mm zone of inhibition at concentration of 500mg/mL. On the other hand, extract from the petroleum ether expressed no antimicrobial activities, based on the absence of the zones of inhibition around the cultures of the respective organisms. Mainly the Dichloromethane and aqueous extracts of E. chlorantha expressed varying organism-dependent MIC on the test isolates. While the lowest MIC of dichloromethane extract was expressed on S. aureus at 6.25mg/mL; the highest MIC (50mg/mL) was exerted on C. albicans. Phytochemical screening of the extracts revealed the presence of glycosides, oxalates, steroids, alkaloids, tannins, flavonoids, phenol, triterpenes, saponin and phytate; while terpenoid was not found in all the plant’s extracts. Findings in this study suggest that the bioactive ingredients of E. chlorantha barks could be best extracted by polar solvents which are evident in the superior antimicrobial activities of dichloromethane and aqueous extracts. The non-polar solvents such as n-hexane and petroleum ether are poor extractant, thus necessitating the need to carefully select suitable solvent for the extraction of any plant’s bioactive compounds.

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Section: Qualitative and Quantitative Phytochemical Analysismentioning

confidence: 99%