Phytochemical Screening, Antioxidant and Antimicrobial Activities of Erodium glaucophyllum (L.) L'Hérit

Abdelkebir, Radhia; Najjaa, Hanen; Abdelkarim, Ben Arfa; Mohamed, Neffati

doi:10.4172/2254-609x.100092

Cited by 8 publications

(10 citation statements)

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“…We identified the presence of phytochemical classes in freshly prepared crude extracts of the fruits of S. edule using standard colorimetric procedures [ 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Sechium edule fruit attenuation impact on the cardiomyopathy of the STZ-induced diabetic rats

Mohammad,

Das,

Samanta

et al. 2024

Heliyon

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“…We identified the presence of phytochemical classes in freshly prepared crude extracts of the fruits of S. edule using standard colorimetric procedures [ 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Sechium edule fruit attenuation impact on the cardiomyopathy of the STZ-induced diabetic rats

Mohammad,

Das,

Samanta

et al. 2024

Heliyon

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“…We identified the presence of phytochemical classes in freshly prepared crude extracts of the flowering aerial parts of H. ramosissimum using standard colorimetric procedures 18 , 19 .…”

Section: Methodsmentioning

confidence: 99%

Heliotropium ramosissimum metabolic profiling, in silico and in vitro evaluation with potent selective cytotoxicity against colorectal carcinoma

Fayed

Abouelela

Refaey

2022

Sci Rep

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Heliotropium is a genus of the Boraginaceae family. Its members are used in many traditional and folklore medicines to treat several ailments. Despite this widespread usage, only a few evidence-based scientific studies investigated and identified its phytoconstituents. Herein, we documented the chemical profile of the Heliotropium ramosissimum methanolic extract using gas chromatography-mass spectrometry (GC–MS) and liquid chromatography-tandem mass spectrometry (LC–ESI–MS/MS) and assessed its antioxidant and cytotoxic effects. The methanolic extract exhibited high phenolic content (179.74 ± 0.58 µg/mL) and high flavonoid content (53.18 ± 0.60 µg/mL). The GC–MS analysis of the lipoidal matter allowed us to identify 41 compounds with high percentages of 1,2-benzenedicarboxylic acid, bis(2-methoxyethyl) ester (23.91%), and 6,10,14-trimethylpentadecan-2-one (18.74%). Thirty-two phytomolecules were tentatively identified from the methanolic extract of H. ramosissimum using LC–MS/MS. These compounds belonged to several phytochemical classes such as phenolic acids, alkaloids, coumarins, and flavonoids. Furthermore, we assessed the antioxidant activity of the methanolic extract by DPPH assay and oxygen radical absorbance capacity assay, which yielded IC50 values of 414.30 µg/mL and 170.03 ± 44.40 µM TE/equivalent, respectively. We also assessed the cytotoxicity of the methanolic extract on seven different cell lines; Colo-205, A-375, HeLa, HepG-2, H-460, and OEC showed that it selectively killed cancer cells with particularly potent cytotoxicity against Colo-205 without affecting normal cells. Further studies revealed that the extract induced apoptosis and/or necrosis on Colo-205 cell line at an IC50 of 18.60 µg/mL. Finally, we conducted molecular docking on the LC–ESI–MS/MS-identified compounds against colon cancer antigen 10 to find potentially cytotoxic compounds. Binding score energy analysis showed that isochlorogenic acid and orientin had the highest affinity for the colon cancer antigen 10 protein, with binding scores of (− 13.2001) and (− 13.5655) kcal/mol, respectively. These findings suggest that Heliotropium ramosissimum contains potent therapeutic candidates for colorectal cancer treatment.

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“…In a test tube, 5 ml of extract was mixed with 2 ml of dichloromethane, and then 3 ml of Conc H2SO4 was added to form a layer an aqueous layer. A reddish-brown coloration forms at the interface indicating the presence of terpenoids 16 .…”

Section: Terpenoids: Salkowski Testmentioning

confidence: 99%

Phytochemical, Anatomical, and Histochemical Investigation of Felicia abyssinica: A Chemical Analysis of Tissues in Relation to Their Structural Organization

Elshorabgy,

El-Banna,

Fayed

et al. 2023

Journal of Advanced Medical and Pharmaceutical Research

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The genus Felicia, family Asteraceae, is used in South African folk medicine. The Zulu and Xhosa healers prescribed the aqueous extract of Felicia species to treat headaches, fever, stomach diseases, and catarrh due to its analgesic, anthelmintic, antibacterial, antifungal, and anti-inflammatory properties. This study examines Felicia abyssinica aerial parts to disclose the chemical nature of its secondary metabolites as well as their histochemical localization. The freshly prepared extracts were qualitatively examined to detect phytochemical constituents using different chemical reagents. Selected plant sections and powdered parts were investigated using a light microscope. The transverse sections of stems and leaves were stained with chemical reagents specific for detecting various phytoconstituents. We detected high amounts of nitrogenous compounds, flavonoids, phenolic compounds, steroids, and low quantities of carbohydrates, glycosides, mucilage, and saponins. The leaves showed anomocytic and anisocytic stomata, one layer of epidermis, dorsiventral mesophyll, and a collateral vascular bundle. The multicellular, uniseriate, non-glandular trichomes with tapered apical cells are characteristic of the leaves. The stem shows a multicellular uniseriate stalk with multicellular head glandular trichomes. In the leaves, nitrogenous compounds were localized in the mesophyll, phenolics, and lignins in the xylem vessels, while flavonoids in the epidermis and xylem. However, in the stem, nitrogenous compounds, flavonoids, and lignins were found in hypodermis; and the area of vascular bundles, including xylem vessels and fibers. This study for the first time, investigates the anatomy of F. abyssinica L aerial parts and also correlates the phytochemical analysis with the structural organization of F. abyssinica tissues.

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Phytochemical Screening, Antioxidant and Antimicrobial Activities of Erodium glaucophyllum (L.) L'Hérit

Cited by 8 publications

References 0 publications

Evaluation of Sechium edule fruit attenuation impact on the cardiomyopathy of the STZ-induced diabetic rats

Evaluation of Sechium edule fruit attenuation impact on the cardiomyopathy of the STZ-induced diabetic rats

Heliotropium ramosissimum metabolic profiling, in silico and in vitro evaluation with potent selective cytotoxicity against colorectal carcinoma

Phytochemical, Anatomical, and Histochemical Investigation of Felicia abyssinica: A Chemical Analysis of Tissues in Relation to Their Structural Organization

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