Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Sai, Kusum; Thapa, Raj Kumar; Devkota, Hari Prasad; Joshi, Khem Raj

doi:10.3390/medicines6020070

Cited by 28 publications

(29 citation statements)

References 34 publications

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“…It is worth mentioning here that the plants with higher exposure to UV-B triggered phenylalanine ammonia-lyase. Phenylalanine ammonia-lyase is the key enzyme in the biosynthesis of the most important secondary metabolites [77], although these metabolites play a crucial role in plant adaptation to their adverse environmental conditions [78,79]. In addition to the adaptive function of SMs under adverse environments, it plays a crucial role in the maintenance of the stability of the primary metabolism, protecting it from disturbance induced by accumulation of specific metabolites via its conversion into secondary metabolites [80].…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content

Hashim

Alharbi

Abdulmajeed

et al. 2020

Plants

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Most endemic plant species have limited altitudinal ranges. At higher altitudes, they are subjected to various environmental stresses. However, these plants use unique defense mechanisms at high altitudes as a convenient survival strategy. The changes in antioxidant defense system and accumulation of different secondary metabolites (SMs) were investigated as depending on altitude in five endemic endangered species (Nepeta septemcrenata, Origanum syriacum subsp. Sinaicum, Phlomis aurea, Rosa arabica, and Silene schimperiana) naturally growing in Saint Katherine protectorate (SKP). Leaves were collected from different sites between 1600 and 2200 m above sea level to assess the biochemical and physiological variations in response to high altitudes. At higher altitudes, the soil pH and micronutrient soil content decreased, which can be attributed to lower mineralization processes at lower pH. Total phenols, ascorbic acid, proline, flavonoids, and tannins increased in response to different altitudes. SMs progressively increased in the studied species, associated with a significant decrease in the levels of antioxidant enzyme activity. R. arabica, as the most threatened plant, showed the maximum response compared with other species. There was an increase in photosynthetic pigments, which was attained via the increase in chlorophyll a, chlorophyll b, and carotenoid contents. There was a significant increase in total soluble sugars and total soluble protein content in response to different altitudes. SDS-PAGE of leaf proteins showed alteration in the protein profile between different species and the same species grown at a different altitude. These five species can adapt to high-altitude habitats by various physiological mechanisms, which can provide a theoretical basis for the future conservation of these endangered endemic species in SKP.

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Section: Discussionmentioning

confidence: 99%

Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content

Hashim

Alharbi

Abdulmajeed

et al. 2020

Plants

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“…The extracts of VMF‐WF and biscuits were prepared based on the technique of Sai, Thapa, Devkota, and Joshi (2019). The VMF‐WF, control, and VMF biscuits powder were added to 10 ml of 80% acetone at the concentration of 60, 125, 185, and 250 mg/ml, and then, samples were prepared and analyzed as described by Sai et al (2019).…”

Section: Methodsmentioning

confidence: 99%

Utilization of Vigna mungo flour as fat mimetic in biscuits: Its impact on antioxidant profile, polyphenolic content, storage stability, and quality attributes

Saeed

Ali

et al. 2020

Legume Science

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In the present study, an effort was made to incorporate Vigna mungo flour (VMF) as a fat mimetic for the development of functional biscuits. Rheological properties of dough, pasting profile, total phenolic content (TPC) and total flavonoid content (TFC), antioxidant activities (1, 1‐diphenyl‐2‐picrylhydrazyl [DPPH] inhibition and ferric reducing antioxidant power [FRAP]), shelf life, and nutritional and sensory properties of the samples were analyzed. An increase in DPPH inhibition, FRAP, TPC, and TFC was observed with increase in the concentration of VMF in wheat flour (WF). The addition of VMF resulted in increased water absorption (58.71–72.40%) and dough stability time (6.30–13.11 min). Set back viscosities (479–81 torque) decreased significantly (P ≤ 0.05), whereas break down viscosities (378–447 torque) increased. The baking process decreased the IC50 values of DPPH inhibition (255.40–169.21 mg/ml) and FRAP (114.45–34.12 mg/ml), although TPC and TFC decreased. The study of shelf life for 2 months revealed no significant difference (P ≤ 0.05) in antioxidant, phenolics, and flavonoids contents of VMF‐WF biscuits. Furthermore, the antioxidants, TFC, and TPC compounds were undetected in control throughout the storage period. The increase in both peroxide value (3.9 mEqO2/kg of fat) and free fatty acid (0.87%) was significantly lower (P ≤ 0.05) than that of the control biscuits during storage but were in an acceptable range. It correlates to the enhanced activity of natural antioxidants as TPC and TFC in VMF‐WF biscuits, which are responsible for the decrease in the rate of oxidation. Nutritional data of VMF‐WF biscuits revealed that protein (11.20–15.85%), crude fiber (0.21–2.01%), and ash content (0.53–4.01%) significantly increased (P ≤ 0.05) and calories (497–430 kcal/100 g) reduced because of the addition of fat mimetic. Biscuits prepared with VMF 15% demonstrated superior quality as compared with control. In conclusion, it could be suggested that the addition of VMF as a fat mimetic in biscuits may increase antioxidant and polyphenolic contents with enhanced storage stability. The reduced calories, increased protein, fiber, mineral contents, and enhanced antioxidant activity considerably increase the nutritional status of the biscuits.

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“…After drying, they were ground by an electric mixer until they became a powder. Then the powdered samples were stored in a dark place and subjected to extraction method (Sai et al, 2019). Extraction of powdered samples was done using aqueous ethanol (80%).…”

Section: Preparation Of Plant Extractmentioning

confidence: 99%

Anthelmintic and Antioxidant activity of Aqueous Ethanolic Extract of Erythrina subumbrans (Hassk.) Merr.

Ganesh¹,

M²

2020

ijrps

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Aqueous Ethanolic extract of vegetative (leaf) part of herbal plant Erythrina subumbrans (Hassk.) was assessed for Anthelmintic and Antioxidant activity. The obtained crude extract was prepared in different concentration, i.e., 50 and 100 mg/mL, against the standard Piperazine citrate, i.e., 10mg/mL for anthelmintic activity. Pheretima posthuma test worms were used during the study and the anthelmintic activity was evaluated based on Paralysis and Death time. Three groups of the test specimen and freshly prepared solutions were used during the study. The average paralysis and death time of standard is 1.4 & 39.59 min (10mg/mL) compared to sample 5.3 & 65.09 min (50mg/mL); 2.5 & 54.08 min (100mg/mL). From the data, aqueous ethanolic extract possesses promising anthelmintic activity compared to piperazine citrate standard. Similarly, antioxidant activity was evaluated for sample at different concentration ranging from 5, 10, 20, 40, 80, 160, and 320 µg/ml premixed with 2.5mL of 0.0135mM DPPH solution. Ascorbic acid was used as a standard and treated in a similar way as that of a sample. Control samples were also prepared without standard and sample solutions. Prepared solutions were vortexed and kept at benchtop condition for 30 minutes and absorbance was measured at 517 nm in a UV-Vis spectrophotometer. The absorbance pattern of the sample is not comparable with that of the standard solution at the same concentration. Aqueous ethanolic extract with IC50 (half maximal inhibitory concentration) value is >320 µg/mL against standard Ascorbic acid with an IC50 value of 15.5 µg/ml. Which witness the crude extract possess less antioxidant activity.

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Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Cited by 28 publications

References 34 publications

Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content

Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content

Utilization of Vigna mungo flour as fat mimetic in biscuits: Its impact on antioxidant profile, polyphenolic content, storage stability, and quality attributes

Anthelmintic and Antioxidant activity of Aqueous Ethanolic Extract of Erythrina subumbrans (Hassk.) Merr.

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