Phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis of phytochemical constituents and anti-bacterial activity of Aerva lanata (L.) leaves

Thangavel, Arun; Balakrishnan, Senthilkumar; Arumugam, Aarthy; Duraisamy, Senbagam; Sureshkumar, Muthusamy

doi:10.5897/ajpp2013.3969

Cited by 14 publications

(3 citation statements)

References 18 publications

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“…GC/MS analysis was conducted following Thangavel et al, method with modifications . For GC/MS, powdered R.…”

Section: Methodsmentioning

confidence: 99%

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Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Ishtiaq,

Ahmad,

Zahid

et al. 2024

ACS Omega

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Ranunculus hirtellus, also known as crowfoot (buttercup), has a rich tradition of use in various biological contexts. While antibacterial studies on extracts from this plant have been conducted, the phytochemical composition, antioxidant properties, and antidiabetic effects remain unexplored. In this study, the phytochemical, antioxidant, and antidiabetic effects of its methanol and aqueous extracts were investigated. Our approach involved gas chromatography− mass spectrometry (GC/MS), alongside quantitative and qualitative methods, for phytochemical profiles. Additionally, concerning biological activities, the antioxidant effect was assessed through 2, 2-diphenyl-pieryl hydrazyl (DPPH) and 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) assays, while the antidiabetic effect was examined through the α-amylase inhibitory assay. The chloroform, ethyl acetate, and n-hexane extracts of R. hirtellus revealed the presence of 14 distinct compounds. In the methanol extract, sterols, quinones, glycosides, lactones, lignin, and flavonoids were identified. The aqueous extract contained sterols, alkaloids, glycosides, triterpenes, terpenoids, quinones, leucoanthocyanins, and lactones. The total flavonoid content (TFC), total phenolic content (TPC), total tannin content (TTC), and reducing sugar content (RDC) were determined in plant extracts, and a linear relationship was found between these parameters. Additionally, the TTC, TPC, and TFC values for both extracts hovered around 0.3786, 0.0476, and 0.1864 μg/mL, respectively, across all plant concentrations, while RDC ranged from 0.9336 to 1.0119 μg/mL in all four extracts. In vitro assays demonstrated dose-dependent antidiabetic activity in both methanolic and aqueous extracts by inhibiting α-amylase. Furthermore, the antioxidant activity observed in the DPPH assay was greater in the aqueous extract compared with the methanolic extract. In addition, the ethyl acetate extract exhibited the highest inhibition among chloroform and n-hexane in the ABTS assay. The results suggest that R. hirtellus can be a potential source of natural antioxidants and antidiabetic agents, and further studies are warranted to investigate the underlying mechanisms of its therapeutic effects.

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“…GC/MS analysis was conducted following Thangavel et al, method with modifications . For GC/MS, powdered R.…”

Section: Methodsmentioning

confidence: 99%

“…GC/MS analysis was conducted following Thangavel et al, method with modifications. 32 For GC/MS, powdered R. hirtellus (200 g) was mixed with 500 mL of solvent. To prepare the samples, 20 g of R. hirtellus powders (aerial parts and roots) were used separately.…”

Section: Methodsmentioning

confidence: 99%

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Ishtiaq,

Ahmad,

Zahid

et al. 2024

ACS Omega

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“…The identification of the Tp EtOH70% leaf extract compounds was performed using a trace gas chromatograph interfaced to a Polaris Q mass spectrometer (Thermo Finnigan, Hertfordshire, UK). According to Thangavel et al (2014), DB5-MS capillary standard non-polar column (30 m × 0.25 mm × 0.25 µm) and helium as a carrier gas were used at a constant flow rate of 1 ml • min -1 . The temperature of the oven was kept at 70°C and adjusted to reach 260°C at an increasing rate of 6°C/min for 2 min.…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysis Of Tp ...mentioning

confidence: 99%

Journal of Plant Protection Research

Ismail¹,

Tag²,

Rizk³

2019

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This study investigates the acaricidal, ovicidal, and repellent effects of the Tagetes patula Linn. (Asteraceae) leaf extract against both the adult female and egg stages of Tetranychus urticae Koch (Trombidiformes: Tetranychidae) under laboratory conditions. The Tagetes patula ethanolic leaf extract [Tp EtOH70% ] was screened for adulticide and ovicide bioassays in order to consider its acute toxicity. One sublethal concentration was used to assess egg-laying capacity (fecundity), repellent, and oviposition deterrent activities. The chemical characterization was conducted by gas chromatography-mass spectrometry (GC-MS) analysis to identify the Tp EtOH70% bioactive components. Results showed that the LC 50 value of Tp EtOH70% leaf extract predicted by Probit analysis against T. urticae adult females at 24 h was 0.99%. The Tp EtOH70% leaf extract showed a significant toxic effect as the highest mean mortality rates (± SE) of the treated adult females was 88.9 ± 3.7%. However, the Tp EtOH70% leaf extract was insignificant in affecting the egg-laying capacity of the adult females treated with a sublethal dose of 0.5% even after 72 h. The Tp EtOH70% leaf extract was classified repellent since the repellent index (RI) value was lower than 1 -SD. In addition, it had a high oviposition deterring effect based on a 100% reduction of the total number of eggs. The Tp EtOH70% leaf extract had a significant ovicidal effect on T. urticae eggs, with 56.04% reduction in hatching. Five bioactive compounds from various classes of phytochemicals were identified in the Tp EtOH70% leaf extract and the major compound was phytol (62.72%). This pioneering investigation reveals the adulticidal, ovicidal, and repellent activities of the Tp EtOH70% leaf extract against T. urticae. A combination of multiple modes of action of different plant components may act alone or in synergism to delay the development of mite resistance.

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A review on phytoestrogens: Current status and future direction

Patra

Gorai

Pal

et al. 2023

Phytotherapy Research

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Phytoestrogens are plant secondary metabolite that is structurally and functionally similar to mammalian estrogens, which have been shown to have various health benefits in humans. Isoflavones, coumestans, and lignans are the three major bioactive classes of phytoestrogens. It has a complicated mechanism of action involving an interaction with the nuclear estrogen receptor isoforms ERα and ERβ, with estrogen agonist and estrogen antagonist effects. Depending on their concentration and bioavailability in various plant sources, phytoestrogens can act as estrogen agonist or antagonists. Menopausal vasomotor symptoms, breast cancer, cardiovascular disease, prostate cancer, menopausal symptoms, and osteoporosis/bone health have all been studied using phytoestrogens as an additional standard hormone supplemental remedy. The botanical sources, techniques of identification, classification, side effects, clinical implications, pharmacological and therapeutic effects of their proposed mode of action, safety issues, and future directions for phytoestrogens have all been highlighted in this review.

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Phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis of phytochemical constituents and anti-bacterial activity of Aerva lanata (L.) leaves

Cited by 14 publications

References 18 publications

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Phytochemicals, Antioxidant, and Antidiabetic Effects of Ranunculus hirtellus Aerial Parts and Roots: Methanol and Aqueous Extracts

Journal of Plant Protection Research

A review on phytoestrogens: Current status and future direction

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