Phytochemical Screening, Proximate Composition and Antibacterial Activity of Oyster Mushroom, Pleurotus ostreatus Collected from Etim Ekpo in Akwa Ibom State, Nigeria

Ikon, Grace Michael; Udobre, E. A.; Etang, U. E.; Ekanemesang, U; Ebana, R. U. B.; Edet, U. O.

doi:10.9734/afsj/2019/45956

Cited by 7 publications

(6 citation statements)

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“…Molisch's, Barfoed's, and Benedict's tests yielded positive results signifying that it is a carbohydrate. The results obtained were in accordance with previous literatures [29][30][31]. Since β-glucan is a non-starch polysaccharide, it is crucial to test the absence of starch to continue working for large-scale industrial production of valueadded milk.…”

Section: Qualitative Testssupporting

confidence: 90%

Fortification of soy milk with prebiotic natural ß-glucan derived from edible mushrooms Pleurotus ostreatus and Agaricus bisporus

Kumaresan¹,

Gopalakrishnan²,

K.³

et al. 2022

J Appl Biol Biotech

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Hot water extraction of edible mushrooms Pleurotus ostreatus and Agaricus bisporus at 90°C for 4 hours yielded a polysaccharide fraction containing crude β-glucan. Qualitative and quantitative analyses of the samples were carried out to identify the presence and abundance of carbohydrates. Diethylaminoethyl (DEAE) Sephacel column chromatography followed by affinity chromatography in an agarose-bound Concanavalin A column resulted in purified β-glucan. β-glucan was confirmed through the presence of unique functional groups present in it by using the Fourier transform infrared spectrum technique. Soy milk fortified with β-glucan was formulated, and this can alleviate the rate of obesity and related diseases caused by high calorific carbonated beverages. The further scope of the study involves sensory analysis and commercialization of the product.

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Section: Qualitative Testssupporting

confidence: 90%

Fortification of soy milk with prebiotic natural ß-glucan derived from edible mushrooms Pleurotus ostreatus and Agaricus bisporus

Kumaresan¹,

Gopalakrishnan²,

K.³

et al. 2022

J Appl Biol Biotech

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“…The presence of some vitamins such as Vitamin A, Vitamin C and B-carotene is an indication that C. rotundus may play a part in the regulation of bodily functions, because some vitamins require fat for their dissolution into the blood stream and provide nutrients. Also, vitamin A is necessary for enhancement of good vision that can be gotten from C. rotundus [33,34].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Phytochemical Contents, Proximate Nutritional Composition and Antimicrobial Activity of the Leaves and Rhizome Extracts of Cyperus rotundus Linn. in Uyo, Akwa Ibom State, Nigeria

Ikon

Etang

Udoiko

et al. 2020

SAJRM

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Background: The leaves and rhizome extracts of Cyperus rotundus Linn. popularly called “Nut grass” in many Nigerian communities have been extensively used in local food preparation and in treatment purposes. Aim: This study aimed to evaluate the phytochemical contents, proximate nutritional composition and antimicrobial activity of the leaves and rhizome extracts of C. rotundus. Methodology: The disease-free plant materials were collected from a farm in Uyo, Akwa Ibom State, Nigeria. Preparation of the plant material, methanolic and aqueous extracts; bacterial culture, isolation, microscopy and biochemical identification; phytochemical screening and proximate nutritional analysis were done according to standard methods, while screening for antimicrobial activity was done by agar well diffusion technique. Results: The preliminary phytochemical analysis of the plant extracts showed the presence of bioactive compounds at varying amounts such as glycosides, tannins, reducing sugars, alkaloids, flavonoids, polyphenols, terpenoids, saponins and phlobatannins. The proximate nutritional and elemental analysis of C. rotundus extracts showed high presence of B-carotene (164.3 ± 0.02), Vitamin A (109.25 ± 0.01) and carbohydrate (59.0 ± 0.01) with moderate content of lipid (24.25 ± 0.02) and moisture (9.10 ± 0.01) as well as contents of some mineral elements such as Ca, K and P occurring in the range literature values in mg per 100 g dry weight of the plant sample. The methanol and aqueous extracts of C. rotundus showed varying diameter of zones of inhibition on the test organism. The observable inhibitory effect of the plant extracts on the test organism was more pronounced with methanol extracts as indicated by the diameter of zones of inhibition in mm in the order of 22.0>14.0>13.0 for P. mirabilis, E. coli and S. aureus, respectively compared to the aqueous extract. Conclusion: The results of this study have shown the antimicrobial, therapeutic and nutritional potential of the leaves and rhizome extracts of C. rotundus. It could possibly find application as a good alternative antibacterial agent, nutraceuticals and dietary supplements.

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“…A mushroom is defined as the fleshy spore carrying the fructification of the mushroom which may be epigenic or hypogen once formed and big enough to be seen to the naked eye and plucked by hand. [2] The mushroom has a broad, oyster, or fan-shaped cap. The gills of the mushroom are cream to white.…”

Section: Introductionmentioning

confidence: 99%

“…It is used in cosmetic products for wrinkles, uneven tone, fine lines, and texture because it has potent antioxidant and anti-inflammatory activity. [2,[5][6][7] They are a good source of prebiotics and contain short-chain sugars such as galactose, glucose, fructose, and N-acetylglucosamine. The bioactive components that are present in mushrooms contain peptides, polysaccharides, lipopolysaccharides, nucleosides, glycoproteins, lectins, lipids, and triterpenoids.…”

Section: Introductionmentioning

confidence: 99%

Pharmacognostic, Chemical Characterization Studies on Oyster Mushroom (Pleurotus ostreatus)

Kadam,

Giram,

Shaikh

et al. 2023

Pharmacogn. Res.

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Aim:The purpose of this study is to determine the Pharmacognostic, phytochemical and physicochemical standardization of Pleurotus ostreatus. Materials and Methods: The disease-free oyster mushroom fruiting body was collected during the rainy season from Himgiree Hi-Tech Agro (Mushroom Division) Kalyani Nagar Pune. Their identity and authentication were done. Macromorphology, cytomorphology, powder microscopy, micrometry, physicochemical evaluation, and phytochemical screening were done according to the WHO guidelines and standard methods. Results: Macromorphology of mushroom structure has a broad, oyster or fan-shaped cap, spanning 2-35 cm. yellowish grey colour, chocolaty odor, and significant taste. Cytomorphology of mushroom shows different cells such as aciculate, sphaerodunculate, lanceolate thick-walled, utriform, cornuate, Subcapitate, mucronate, cornuate, clavate 4-sterigmate basidium and in powder microscopy nodulose, isodiametric, snout like pore, spiny, bullet shaped, cuboid. The preliminary phytochemical analysis of the mushroom shows the occurrence of active compounds such as flavonoid, tannin, glycoside, coumarin, phenols, steroid, terpenoids protein and amino acid. Conclusion: The results obtained in this study the Standardization of phytochemical, physico-chemical is a fundamental dimension for sample identification, quality, and purity of oyster mushroom (Pleurotus ostreatus).

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Phytochemical Screening, Proximate Composition and Antibacterial Activity of Oyster Mushroom, Pleurotus ostreatus Collected from Etim Ekpo in Akwa Ibom State, Nigeria

Cited by 7 publications

References 0 publications

Fortification of soy milk with prebiotic natural ß-glucan derived from edible mushrooms Pleurotus ostreatus and Agaricus bisporus

Fortification of soy milk with prebiotic natural ß-glucan derived from edible mushrooms Pleurotus ostreatus and Agaricus bisporus

Evaluation of Phytochemical Contents, Proximate Nutritional Composition and Antimicrobial Activity of the Leaves and Rhizome Extracts of Cyperus rotundus Linn. in Uyo, Akwa Ibom State, Nigeria

Pharmacognostic, Chemical Characterization Studies on Oyster Mushroom (Pleurotus ostreatus)

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