Phytochemical studies and antibacterial activity of <b> <i>Centaurium pulchellum</i> </b> Druce

Bibi, Hafsa; Ali, İrshad; Sadozai, S. K.; Atta-ur-Rahman, _

doi:10.1080/14786410500162047

Cited by 13 publications

(4 citation statements)

References 12 publications

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“…All the tested extracts possessed antibacterial activity with MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations) in the range of 0.05-0.2 mg mL -1 , depending on the bacterial species (Table 1), showing similar effects as the commercial antibiotics Streptomycin and Ampicillin. These results indicate that methanol extracts of C. pulchellum were more powerful antibacterial agent than previously reported for chloroform, ethyl acetate and ethanol extracts of this species [3]. Despite the fact that selected populations significantly differ in their quantitative secoiridoid content, no major differences in antibacterial activities were observed.…”

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confidence: 56%

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Antibacterial and Antifungal Screening of Centaurium Pulchellum Crude Extracts and Main Secoiridoid Compounds

Šiler

Mišić

Nestorović

et al. 2010

Natural Product Communications

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The main principles of C. pulchellum (Sw.) Druce, secoiridoid glycosides, have been studied as potent bioactive compounds. Here we scored their content in extracts of ten populations of this species. Antibacterial and antifungal assays of the extracts and pure secoiridoid glycosides were performed against eight bacterial strains and five fungal species. Methanol extracts from both aerial parts and roots exhibited excellent antibacterial (0.05-0.2 mg mL-1) and very good antifungal (0.1-2 mg mL-1) activity. Pure secoiridoid glycosides isolated from these extracts demonstrated very strong antibacterial (0.01-0.04 mg mL-1) and especially antifungal (0.001-0.1 mg mL-1) activity.

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confidence: 56%

“…The traditional medicine of Egypt recommends this species for the treatment of various disorders [1]. This annual herbaceous plant, like the majority of Centaurium species, is a rich source of bitter secoiridoid glycosides, xanthones and β-monoglycosides [2] and is reported to have some antibacterial properties [3].…”

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confidence: 99%

Antibacterial and Antifungal Screening of Centaurium Pulchellum Crude Extracts and Main Secoiridoid Compounds

Šiler

Mišić

Nestorović

et al. 2010

Natural Product Communications

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“…By using the well diffusion method, the crude ethyl acetate extracts of endophytic fungi suspended in Dimethyl Sulfoxide (DMSO) were screened against bacterial pathogens, and the zones of inhibition were measured [ 22 , 23 ]. Amoxicillin was used as positive control, while dimethyl sulfoxide (DMSO) was used as negative control.…”

Section: Methodsmentioning

confidence: 99%

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Rehman

Khan

Hamayun

et al. 2022

BioMed Research International

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Plant species are known to harbor large number of endophytes, which stays in plant tissues as symbionts. These endophytes secrete large array of bioactive compounds that have potency against certain diseases with no side effects. We have collected leaf samples of the Moringa oleifera plant from the Pakistan Forest Institute, Khyber Pakhtunkhwa, Pakistan for the isolation of beneficial endophytes. The strains isolated from the leaves of M. oleifera were coded with MOL and tested for antimicrobial, antifungal, germicidal, phytotoxic, insecticidal, cytotoxic, and anti-inflammatory activities. The isolates, MOL1, MOL16, MOL19, and MOL21, possessed antibacterial activity against Staphylococcus aureus, whereas MOL7 inhibited 55% of the growth of Escherichia coli. MOL3 inhibited the growth of E. coli, S. aureus, and Pseudomonas aeruginosa. The strains, MOL1 and MOL7, showed antifungal activity against Candida albicans and Saccharomyces cerevisiae, while the strains, MOL11 and MOL17, showed activity against Verticillium chlamydosporium. The isolates, MOL3, MOL7, MOL9, MOL15, MOL17, MOL18, and MOL19, inhibited the growth of Lemna minor (duckweed) at 100 μg/ml. MOL2 exhibited strong activity in the brine shrimp assay, while MOL1, MOL2, MOL5, MOL6, MOL12, MOL17, MOL19, and MOL20 showed insecticidal, and MOL3 demonstrated larvicidal and antileishmanial activity. The isolated potent endophytes were identified as Aspergillus, Penicillium, Fusarium, Tricoderma, Rhizoctonia, Mucor, Alternaria, Pestalotiopsis, Acremonium, and Cladosporium through morphological and microscopic characteristics of the colonies.

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“…Chemotaxonomy and pharmacology study reports that swertiamarin is usually found in Gentianaceae family (Jensen, & Schripsema, 2002). It was found that many plants in Gentianaceae family containing a secoiridoid glycoside, swertiamarin possessed antibacterial properties such as Centaurium species (Bibi et al, 2006;van der Sluis, 1985). and Enicostema species (Perumal et al, 2022;Pillai et al, 2020;Vishwakarma et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Swertiamarin Content from Fagraea fragrans Leaf Extract using HPLC Technique and its Correlation to Antibacterial Activity

Hongsing,

Kongart,

Nuiden

et al. 2024

JCST

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The rise in drug resistance poses escalating challenges for antibacterial medications, leading to an urgent demand for the exploration and innovation of new antibacterial drugs. Fagraea fragrans Roxb., belonging to the Gentianaceae family, is one of the common herbal medicines which can be found abundantly in Southeast Asia. The secoiridoid glucoside swertiamarin, one of the major compounds in F. fragrans leaf, exhibits antimicrobial effects. To guarantee the medicinal effectiveness of F. fragrans leaves, it is essential to identify a standardized analytical method for quantifying the active compound. In this study, the optimized HPLC method following ICH guideline was validated for the quantitative analysis of swertiamarin content in F. fragrans leaf in terms of linearity (y = 5733.5x - 369.1; R2 = 0.9999), accuracy (93.57-96.39% recovery), precision (0.91% RSD for repeatability precision; 1.19% RSD for intermediate precision), limit of detection (0.73 µg/mL), limit of quantitation (2.23 µg/mL), specificity (peak purity index = 0.999995) and robustness (% RSD <1).The maximum wavelength of swertiamarin was found to be at 238 nm. The amount of swertiamarin content in F. fragrans leaf extract conducted from the validated HPLC method was found to be 0.0259 ± 0.0005 g/100 g crude drug. The leaf extract exhibited antimicrobial activity against clinical isolates of Enterobacter cloacae, Klebsiella pneumonia, and Escherichia coli at 0.125 mg/mL, and Pseudomonas aeruginosa at 0.5, showing minimum inhibitory concentration (MIC) values. Whereas swertiamarin exhibited even lower MIC values. The developed HPLC analysis effectively determines swertiamarin content as a chemical marker to ensure the antimicrobial potential of F. fragrans leaves.

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Phytochemical studies and antibacterial activity of Centaurium pulchellum Druce

Cited by 13 publications

References 12 publications

Antibacterial and Antifungal Screening of Centaurium Pulchellum Crude Extracts and Main Secoiridoid Compounds

Antibacterial and Antifungal Screening of Centaurium Pulchellum Crude Extracts and Main Secoiridoid Compounds

Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Quantitative Analysis of Swertiamarin Content from Fagraea fragrans Leaf Extract using HPLC Technique and its Correlation to Antibacterial Activity

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