Phytochemical Studies of the Ethyl Acetate Extract of the Fruit of Piper capense

Abstract

Natural products have been a major source of drugs for centuries. The fruit of Piper capense is used traditionally as spice and for the treatment of infectious diseases in different parts of Ethiopia. The main objective of this work is to carry out phytochemical study of the EtOAc crude extract of the fruit of P. capense. Phytochemical screening on this crude extract revealed the presence of phenols, alkaloids, steroids, terpenes, saponins and flavonoids. After silica gel column chromatography the crude extract led to the isolation of compound PC1 identified as 5-Hydroxy-7, 4'-dimethoxyflavone. Characterization of this compound was achieved via spectroscopic methods (NMR, UV and IR). The structure of compound PC2 was elucidated, the spectral data showed the presence of more than one compound one of which is partially characterized and proposed to be rotenone.

Phytochemical Constituents of Aquilaria malaccensis Leaf Extract and Their Anti-Inflammatory Activity against LPS/IFN-γ-Stimulated RAW 264.7 Cell Line

“…Figure demonstrates 1 H– 1 H COSY, HMQC, and HMBC correlations in compound 2 . The spectral data for compound 2 is in well agreement with 5 - hydroxy-7,4′-dimethoxyflavone reported in ref .…”

“…An absorption band appeared at 285 nm on the UV spectrum. 3, which are similar to aquilarinenside E previously isolated from Aquilaria sinensis leaves 34 and flower buds, 45 compound 6 is identified as aquilarinenside E. 40 The HRESIMS spectrum of compound 7 revealed a significant peak at m/z 391.1002 [M − H] − indicated to the molecular formula of C 19 H 20 O 9 , while the UV spectrum demonstrated a maximum absorption band at 285 nm. IR spectrum revealed absorption bands at 3326, 1635, and 1595 cm −1 recognized as OH, CO, and aromatic groups absorptions, respectively.…”

“…The molecular formula of the compound (C 21 H 22 O 10 ) was in agreement with the molecular ion peak that occurred at m / z 434.1250 [M – H] − analyzed using the HRESIMS technique. Based on the spectroscopic data of the compound presented in Table , which are similar to aquilarinenside E previously isolated from Aquilaria sinensis leaves and flower buds, compound 6 is identified as aquilarinenside E …”

“…From the leaves of A. malaccensis , eight known compounds ( 1 – 8 ) were obtained and structurally characterized (Figure ). Through comparing the NMR spectroscopic data of the isolated compounds with literature values, the compounds were recognized as epifriedelanol ( 1 ), 5-hydroxy-7,4′-dimethoxyflavone ( 2 ), luteolin-7,3′,4′-trimethyl ether ( 3 ), , luteolin 7,4′- dimethyl ether ( 4 ), , acacetin ( 5 ), aquilarinenside E ( 6 ), iriflophenone-2- O -α- l -rhamnopyranoside ( 7 ), and Iriflophenone 3- C -β-glucoside ( 8 ). , The above-mentioned compounds were obtained from the species of A. malaccensis for the first time.…”

Phytochemistry and Cytotoxic Activity of Aquilaria crassna Pericarp on MDA-MB-468 Cell Lines

“…Thus, compound 4 was identified and confirmed as 5-hydroxy-7,4′-dimethoxyflavone by comparison with reported data. 23 The MS (ESI − ), NMR spectroscopic data in DMSO-d 6 suggested that compound 5 was also a flavon derivative with two methoxy groups and two hydroxy groups. Based on HMBC data, the two methoxy groups were attached at C7 and C3′, meanwhile two hydroxy groups were linked at the C5 and C4′ positions.…”