Phytochemical study and evaluation of cytotoxic and genotoxic properties of extracts from Clusia latipes leaves

Bailón-Moscoso, Natalia; Romero-Benavides, Juan Carlos; Sordo, Monserrat; Villacis, Javier; Silva, Ronald; Celi, Luisa; Martínez‐Vázquez, Mariano; Ostrosky‐Wegman, Patricia

doi:10.1016/j.bjp.2015.08.014

Cited by 14 publications

(8 citation statements)

References 25 publications

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“…The cells were incubated at 37 ∘ C in a 5% CO 2 atmosphere. The viable cells were counted using the trypan blue exclusion method in a hemocytometer [8].…”

Section: Cell Culture Procedures Four Human Cancer Cell Linesmentioning

confidence: 99%

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Romero-Benavides

Ortega-Torres

Villacis

et al. 2018

International Journal of Medicinal Chemistry

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Some species of the Baccharis genus have been shown to possess important biomedical properties, including cytotoxic activity. In this study, we examined the cytotoxic effect of methanol extract from Baccharis obtusifolia (Asteraceae) in cancer cell lines of prostate (PC-3), colon (RKO), astrocytoma (D-384), and breast (MCF-7). The methanolic extract displayed the largest substantial cytotoxic effect in lines of colon cancer (RKO) and cerebral astrocytoma (D-384). Chromatographic purification of the B. obtusifolia methanolic extract led to the isolation and identification of 5,4′-dihydroxy-7-methoxyflavone (1) and 5-hydroxy-7,4′-dimethoxyflavone (2) compounds of the flavonoid type.

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“…The cells were incubated at 37 ∘ C in a 5% CO 2 atmosphere. The viable cells were counted using the trypan blue exclusion method in a hemocytometer [8].…”

Section: Cell Culture Procedures Four Human Cancer Cell Linesmentioning

confidence: 99%

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Romero-Benavides

Ortega-Torres

Villacis

et al. 2018

International Journal of Medicinal Chemistry

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“…Different G. neuberthii extracts were evaluated on cell proliferation by the MTS metabolic viability assay, according to Guamán-Ortiz et al (2015). [10] Briefly, 2 × 10 3 cells were seeded in 96-well plates in 100 μL of medium per well and incubated for 24 h. en, cells were treated in triplicate for 48 h either with 50 μg/mL of each extract or 0.3 μg/mL Dxo. Four hours before finishing the treatment, 20 μL of Cell Titer 96 Aqueous One Solution cell proliferation reagent (Promega, USA) was added to each well.…”

Section: Extraction and Isolation Of Compoundsmentioning

confidence: 99%

Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

Guamán-Ortiz

Romero-Benavides

Suárez

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Traditional herbal medicine has become an important alternative in the treatment of various cancer types, including colon cancer, which represents one of the main health problems around the world. Therefore, the search for new therapies to counteract this disease is very active. Grias neuberthii is an endemic plant located in the Ecuadorian Amazon region, which has been used in traditional medicine for its pharmacological properties, including its ability to inhibit tumor cell growth, although scientific studies are limited. We have analyzed the effect of this plant on two colon carcinoma cell lines, that is, RKO (normal p53) and SW613-B3 (mutated p53) cells. Among several extracts obtained from various parts of G. neuberthii plant, we identified the extract with the greatest cytotoxic potential, derived from the stem bark. The cytotoxic effect was similar on both cell lines, thus indicating that it is independent of the status of p53. However, significant differences were observed after the analysis of colony formation, with RKO cells being more sensitive than SW613-B3. No evidence for apoptotic markers was recorded; nevertheless, both cell lines showed signs of autophagy after the treatment, including increased Beclin-1 and LC3-II and decreased p62. Finally, three chemical compounds, possibly responsible for the effect observed in both cell lines, were identified: lupeol (1), 3′-O-methyl ellagic acid 4-O-β-D-rhamnopyranoside (2), and 19-α-hydroxy-asiatic acid monoglucoside (3).

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“…Leaves and stems were processed separately. The pulverized leaves (1540 g) and stems (1608 g) were extracted by static maceration at ambient temperature with hexane (Hex), ethyl acetate (EtOAc), and methanol (MeOH) sequentially during three days with each solvent 18 . The procedure was repeated three times and concentrated on a rotary evaporator (Buchi R210; Switzerland, Flawil) at 50 mbar and 35°C, to yield a total of six extracts: leaf extracts, 71.00 g (Hex-L), 28.52 g (EtOAc-L), and 283.87 g (MeOH-L); and stem extracts, 39.44 g (Hex-S), 44.23 g (EtOAc-S), and 117.23 g (MeOH-S).…”

Section: Extraction and Partitions Of Clusia Latipesmentioning

confidence: 99%

“…The genus Clusia is widely distributed in the tropical and subtropical regions of Central and South America 12 . In the species of this genus, a great variety of biological activities have been found: broad-spectrum antimicrobial activity; chemopreventive cancer effects and antioxidant activity 13 ; anti-inflammatory and anti-hepatotoxic activity and inhibitory action of the human immunodeficiency virus (HIV) [14][15][16] ; and cytotoxic activity 17,18 . In the present study, both the antioxidant and anti-glycemic activity of leaf and stem extracts of C. latipes were evaluated.…”

Section: Introductionmentioning

confidence: 99%

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

Silva-Rivas¹,

Bailón-Moscoso²,

Cartuche³

et al. 2020

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Introduction: The prevalence of diabetes has increased more rapidly in low and middleincome countries than in high-income countries. Type 2 diabetes mellitus (DM2), which is the most common form of diabetes, is caused by the inefficient use of insulin in the body and is characterized by disrupted insulin action or secretion. Also, oxidative stress plays an important role in the development of disease. The goal of this study is to identify the antioxidant and hypoglycemic properties of Clusia latipes, an endemic species of Central and South America. Methods: The antioxidant and hypoglycemic capacity of the extracts (hexane, ethyl acetate, and methanol) of the leaves and stems of Clusia latipes were evaluated. From the most potent extract, the phytochemical study was carried out and fractionated. Antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), while hypoglycemic capacity was measured by alpha-glucosidase inhibition. Results: The extracts with the highest antioxidant capacity are the extracts with the highest α-glucosidase inhibition activity. Inhibitory activity increased in samples extracted with medium polar (ethyl acetate) and polar (methanol) solvents. Phytochemical screening of these extracts revealed the presence of alkaloids, carbohydrates, flavonoids/xanthones, quinones, saponins, and tannins. The highest α-glucosidase inhibitory activity was detected in the ethyl acetate fraction obtained from leaf methanol extract, with a half-maximal inhibitory concentration (IC 50 ) value of 0.90 μg/ml. The major constituent isolated from the same fraction was isoquercitrin.

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Phytochemical study and evaluation of cytotoxic and genotoxic properties of extracts from Clusia latipes leaves

Cited by 14 publications

References 25 publications

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Phytochemical Study and Evaluation of the Cytotoxic Properties of Methanolic Extract from Baccharis obtusifolia

Cytotoxic Property of Grias neuberthii Extract on Human Colon Cancer Cells: A Crucial Role of Autophagy

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

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