Phytochemical Test and Antibacterial Test of Rhizopora apiculata Mangrove-Root against Escherichia coli and Staphylococcus aureus Bacteria

Usman, Usman

doi:10.20961/jkpk.v2i3.11850

Cited by 10 publications

(7 citation statements)

References 1 publication

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“…There are several factors that affect the antibacterial activity in the content of secondary metabolites. Several factors that affect the inhibition of bacterial growth are the selection of media, the solvent used, the thickness of the media, the solvent used and the concentration of the methanol extract used [12]. From this statement, it can be concluded that there were several factors that caused no inhibition zones to be found in the Staphylococcus aureus sample.…”

Section: Figure-2 Inhibition Ability Test Processmentioning

confidence: 99%

Antibacterial test of Rhizophora apiculata extract originated from Mangrove Wonorejo Surabaya against Staphylococcus aureus and Escherichia coli

Widiani,

Aliyah,

Awwalia

et al. 2023

mrcm

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Rhizopora apiculata is a type of mangrove that has the potential as a source of antibacterial. This plant is used as medicine by coastal communities in Indonesia because it contains useful active ingredients. This research aims to study Rhizophora apiculata antibacterial potential against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The samples used were Rhizophora apiculata leaves from Mangrove Wonorejo Surabaya. The research stages include extracting mangrove leaves, media preparation, and testing the antibacterial inhibition zone. The results of the research showed that the antibacterial test with Escherichia coli bacteria had a weak inhibition zone of less than 10 mm with details of a 0.1 ml sample around 5.35 mm and a 0.2 ml sample around 6.78 mm at 24 hour incubation, a 0.1 ml sample measuring around 7.12 mm and the 0.2 ml sample measuring around 6.98 mm at 48 hours incubation, and for the Staphylococcus aureus bacteria there was no zone of inhibition at 24 and 48 hours incubation for both the 0.1 ml and 0.2 ml samples.

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Section: Figure-2 Inhibition Ability Test Processmentioning

confidence: 99%

Antibacterial test of Rhizophora apiculata extract originated from Mangrove Wonorejo Surabaya against Staphylococcus aureus and Escherichia coli

Widiani,

Aliyah,

Awwalia

et al. 2023

mrcm

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“…According to Acacia et al [4], R. apiculata leaves had secondary metabolite compounds in the form of phenols, alkaloids, flavonoids, tannins, saponins and steroids. Usman [31] also said that the roots of R. apiculata contained secondary metabolites in the form of alkaloids, flavonoids and triterpenoids. Meanwhile, according to Berawi & Marini [7], the stems of R. apiculata had secondary metabolites, namely alkaloids, flavonoids and tannins.…”

Section: Oceanography and Fisheries Open Access Journalmentioning

confidence: 99%

“…The toxic category is seen from the LC50 value, which is found in the stems of R. apiculata (BRA) and roots of R. apiculata (ARA), while the leaves of R. apiculata (DRA) do not show toxicity. According to Sari's research [32], R. apiculata mangrove bark extract had an LC50 value of 338.364 mg/l and according to Usman [31], R. apiculata mangrove root extract had an LC50 value of 25.7 mg/l. As well as previous research conducted by Maulana & Sasmito [9], mangrove leaf extract R. ap/l.…”

Section: Exact Toxicity Levelmentioning

confidence: 99%

Antioxidant Activity and Toxicity Test on Mangrove (Rhizophora Apiculata) from Banyuasin Waters, South Sumatra

Hendri

2023

OFOAJ

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determine the content of secondary metabolites [3]. According to Akasia et al. [4], R. apiculata contained phenolic compounds, alkaloids, flavonoids, tannins, saponins and steroids. However, the metabolite compounds contained in R. apiculata mangrove parts such as stems, roots, and leaves have different concentrations.Antioxidants are compounds that can inhibit free radical reactions in the body [5]. Free radicals are molecular atoms that have unpaired electrons and if the molecular atoms present in high amounts in the body, these can cause stress, cancer, diabetes and inflammation [6]. Free radicals can be formed either naturally in the body through normal metabolic processes (metabolic waste) or from external factors such as food, X-rays, air pollutants, and cigarette smoking. The body has the ability to ward off free radicals in small amounts but not in large quantities. One of the methods used to measure the level of antioxidants in samples is the DPPH method. Toxicity is a qualitative term whether there is a damage or not depending on the amount of toxic active compounds absorbed. The results obtained from the toxicity test using the BSLT method have been proven to have a positive correlation with the toxicity of anticancer compounds [3]. The BSLT method uses A. salina as the observed test object.

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“…Ekstrak etanol tidak menunjukkan sifat antibakteri terhadap bakteri uji, sedangkan ekstrak air hanya bersifat antibakteri terhadap S. aureus [4] . Walaupun beberapa ekstrak menunjukkan aktivitas antibakteri, namun ekstrak metanol dari akar tumbuhan ini tidak bersifat antibakteri terhadap E. coli [4,5] .…”

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DAYA HAMBAT EKSTRAK DAUN MANGROVE (Rhizophora apiculata) ASAL PESISIR SUKADANA TERHADAP BAKTERI Escherichia coli (INHIBITION THE LEAF EXTRACT OF Rhizophora apiculata FROM THE SUKADANA COAST AGAINST Escherichia coli)

Wibowo,

Sari,

Ardiningsih

et al. 2023

indonesian

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Rhizophora apiculata is a plant that is widely found in mangrove forests in Sukadana subdistrict, and has the potential to inhibit of Escherichia coli. This research aims to determine the activity of R. apiculata which grows in the Sukadana mangrove forest in inhibiting E. coli. The leaves of R. apiculata were macerated with methanol solvent, partitioned into three fractions (n-hexane fraction, dichloromethane fraction, and methanol fraction), and tested for phytochemical screening. The results of phytochemical screening of R. apiculata leaves were positive for flavonoids, alkaloids, phenolics, terpenoids, tannins, and saponins. The potential of R. apiculata leaf samples in inhibiting bacterial growth was measured by the size of the clear zone in the antibacterial test with the agar well diffusion method that uses variations in concentration, namely 250 mg/mL, 125 mg/mL, and 62.5 mg/mL. Based on the research results, it is known that R. apiculata leaves can inhibit the growth of the test bacteria with a minimum inhibitory concentration for methanol extract of 250 mg/mL. The methanol and dichloromethane fractions had minimum inhibitory concentrations of 125 mg/mL and 250 mg/mL respectively, while the n-hexane fraction did not inhibit at all test concentrations. So it can be concluded that the leaves of R. apiculata have the potential as an alternative for antibacterial active compounds

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Phytochemical Test and Antibacterial Test of Rhizopora apiculata Mangrove-Root against Escherichia coli and Staphylococcus aureus Bacteria

Cited by 10 publications

References 1 publication

Antibacterial test of Rhizophora apiculata extract originated from Mangrove Wonorejo Surabaya against Staphylococcus aureus and Escherichia coli

Antibacterial test of Rhizophora apiculata extract originated from Mangrove Wonorejo Surabaya against Staphylococcus aureus and Escherichia coli

Antioxidant Activity and Toxicity Test on Mangrove (Rhizophora Apiculata) from Banyuasin Waters, South Sumatra

DAYA HAMBAT EKSTRAK DAUN MANGROVE (Rhizophora apiculata) ASAL PESISIR SUKADANA TERHADAP BAKTERI Escherichia coli (INHIBITION THE LEAF EXTRACT OF Rhizophora apiculata FROM THE SUKADANA COAST AGAINST Escherichia coli)

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