Phytochemical Test Methods: Qualitative, Quantitative and Proximate Analysis

Egbuna, Chukwuebuka; Ifemeje, Jonathan C.; Maduako, Maryann Chinenye; Tijjani, Habibu; Udedi, Stanley Chidi; Nwaka, Andrew C.; Ifemeje, Maryjane Oluoma

doi:10.1201/9780429426223-15

Cited by 28 publications

(22 citation statements)

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“…The extracts were then evaporated to dryness using rotary evaporator [8]. Preliminary phytochemical screenings were done according to the methods described by [9][10][11][12][13][14].…”

Section: Phytochemical Analysismentioning

confidence: 99%

Proximate, Phytochemical and Antioxidant Mineral Compositions of Four Different Brands of Tea

Ifemeje

Egbuna

et al. 2020

Adv. J. Grad. Res.

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The comparative studies of the proximate, phytochemical and antioxidant mineral compositions of four different brands of green tea were investigated. The four different brands, Tianshi®, Qualitea®, Slimfit®, and Xiang® were purchased from a supermarket in Ihiala Local Government Area of Anambra State, Nigeria. The samples were pulverized and stored in an airtight container prior to analysis. The proximate composition was analysed using AOAC method while phytochemical compositions of the green teas were investigated using standard methods. The result of the study indicated that the moisture content (15.80±0.01%) and protein content (1.05±0.00%) of Qualitea® was significantly higher (P<0.05) compared to Slimfit and Xiang tea while the fibre content of Xiang tea (80.35±0.03%) was significantly higher (P<0.05) than other tea studied. The phytochemical study showed the presence of flavonoids, tannins, alkaloids, saponins and cardiac glycosides in all the teas studied. However, Qualitea® contains the highest amount of phytochemicals when compared to the other brands of tea investigated. Data obtained from the antioxidant mineral study showed that the four brands of the tea are good sources of antioxidant minerals such as Fe, Zn, Cu, Mn and Se. It is therefore recommended that tea be included in our menu because of their nutritive and health benefits.

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“…The extracts were then evaporated to dryness using rotary evaporator [8]. Preliminary phytochemical screenings were done according to the methods described by [9][10][11][12][13][14].…”

Section: Phytochemical Analysismentioning

confidence: 99%

Proximate, Phytochemical and Antioxidant Mineral Compositions of Four Different Brands of Tea

Ifemeje

Egbuna

et al. 2020

Adv. J. Grad. Res.

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“…The anthocyanin content was first qualitatively identified using ammonia HCl test following a previous procedure [10]. To be specific, 2 mL of the extract was added with 2 mL of 2N HCl and ammonia.…”

Section: Determination Of Total Anthocyanin Contentmentioning

confidence: 99%

Optimization of Total Anthocyanin Content, Stability and Antioxidant Evaluation of the Anthocyanin Extract from Vietnamese Carissa Carandas L. Fruits

Huynh

Pham

et al. 2019

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In this study, the extraction of anthocyanin colorant from karanda fruit (Carissa carandas L.) was carried out and optimized with multiple single factor assays. Selected conditions for yield maximization consisted of ripen fruits with black-purple color, material size of thin slices (1.0–1.5 mm), solvent of EtOH 50%, material/ solvent ratio of 1:3, temperature of 50 °C, extraction time of 45 min, and two extraction cycles. The anthocyanin content in the extract was 277.2 mg/L, which is equivalent to 9.33 mg anthocyanin per gram of dry material. Aqueous solutions of the extract and dried extracts from Carissa carandas fruit were evaluated for stability at two temperature conditions, namely room temperature (30 ± 2 °C) and 45 °C. The temperature exerted great impact on color change, anthocyanin content and the degree of polymerization of anthocyanin. Aqueous solutions of extract with citric acid (3.0–5.0 g/L) were generally more color stable and less anthocyanin degradable than those without citric acid. In the DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging assay, The half maximal inhibitory concentration (IC50) of the dried extract was 87.56 μg/mL, which was approximately 29 times higher than that of vitamin C. After 3-month storage at −18 °C, IC50 of the dried extract was 173.67 μg/mL.

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“…The isolates were screened for the presence of anthraquinones by Bornträgerʼs test, as described in [33]. Positive reactions gave a pink to violet color of the samples containing anthraquinones but not naphthodianthrones such as hypericin or pseudohypericin (▶ Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The supernatant was transferred to a vial and used for phytochemical screening. The screening reaction for the detection of anthraquinones was performed by Bornträgerʼs test, as described in Egbuna et al [33]. A methanol solution of emodin (≥ 97 %; Sigma Aldrich) was used as a standard in 100 g • dm −3 , 50 g•dm −3 , 25 g •dm −3 , 10 g•dm −3 , and 2 g • dm −3 .…”

Section: Phytochemical Screening For Anthraquinonesmentioning

confidence: 99%

Isolation, Characterization and Targeted Metabolic Evaluation of Endophytic Fungi Harbored in 14 Seed-Derived Hypericum Species

et al. 2020

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Medicinal plants of the genus Hypericum are rich sources of bioactive naphthodianthrones, which are unique in the plant kingdom, but quite common in fungal endophytes. Cultivable endophytic fungi were isolated from 14 different Hypericum spp. originating from seeds grown under in vitro conditions and further acclimated to outdoor conditions. Among 37 fungal isolates yielded from the aerial and underground plant organs, 25 were identified at the species level by the fungal barcode marker internal transcribed spacer rDNA and protein-coding gene region of tef1α. Ten of them were isolated from Hypericum spp. for the first time. The axenic cultures of the isolated endophytes were screened for the production of extracellular enzymes, as well as bioactive naphthodianthrones and their putative precursors by Bornträgerʼs test and HPLC-HRMS. Traces of naphthodianthrones and their intermediates, emodin, emodin anthrone, skyrin, or pseudohypericin, were detected in the fungal mycelia of Acremonium sclerotigenum and Plectosphaerella cucumerina isolated from Hypericum perforatum and Hypericum maculatum, respectively. Traces of emodin, hypericin, and pseudohypericin were released in the broth by Scedosporium apiospermum, P. cucumerina, and Fusarium oxysporum during submerged fermentation. These endophytes were isolated from several hypericin-producing Hypericum spp. Taken together, our results reveal the biosynthetic potential of cultivable endophytic fungi harbored in Hypericum plants as well as evidence of the existence of remarkable plant-endophyte relationships in selected non-native ecological niches. A possible role of the extracellular enzymes in plant secondary metabolism is discussed.

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Phytochemical Test Methods: Qualitative, Quantitative and Proximate Analysis

Cited by 28 publications

References 0 publications

Proximate, Phytochemical and Antioxidant Mineral Compositions of Four Different Brands of Tea

Proximate, Phytochemical and Antioxidant Mineral Compositions of Four Different Brands of Tea

Optimization of Total Anthocyanin Content, Stability and Antioxidant Evaluation of the Anthocyanin Extract from Vietnamese Carissa Carandas L. Fruits

Isolation, Characterization and Targeted Metabolic Evaluation of Endophytic Fungi Harbored in 14 Seed-Derived Hypericum Species

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