Phytochemicals, antimicrobial and antioxidant potential of methanolic extract of Berberis aristata roots

Thakur, Monika; Sharma, Kunjan; Mehta, Sonia; Rai, Swati; Sharma, Isha; Tripathi, Astha

doi:10.5958/0974-360x.2020.01004.5

Cited by 9 publications

(1 citation statement)

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“…BRB in topical delivery dosage form has been reported to have antimicrobial and anti-inflammatory activity as per the result of in vitro and in vivo experiment [10][11][12] . Also, eugenol has been demonstrated as a promising antimicrobial and inflammatory agent by in vitro and in vivo experiment 13,14 . Based on the literature survey, it is hypothesized that the combination of berberine chloride and eugenol in formulated emulgel can be effective in controlled management of S. aureus and Candida albicans induces inflammation and skin infections.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Methods for the Analysis of Berberine Hydrochloride and Eugenol in Formulated Emulgel

Zanwar¹,

Sen²,

Memon³

et al. 2022

JNR

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The present work describes three spectrophotometric methods for determining two phytoconstituent berberine hydrochloride and eugenol in formulated gels: simultaneous equation method, absorbance correction, and zero-crossing derivative method. In the simultaneous equation method, the absorbance at 263 nm and 280 nm and the absorbance correction method at 345 nm and 280 nm were measured and applied to their respective equation for the estimation of berberine hydrochloride and eugenol in phosphate buffer and formulated emulgel. The amplitudes of the first derivative spectra were measured at 252.5 nm for berberine hydrochloride and 263.5 nm for eugenol in zero-crossing crossing derivative spectrophotometry. For berberine hydrochloride and eugenol, linearity was attained in the concentration ranges of 4–20 and 2–10 µg/ml, respectively. Validation shows the applicability of the above procedures for the quantitative determination of berberine hydrochloride and eugenol. As a result, the presented method sucessfully estimated the aforesaid active phytoconstituent in formulated emulgel, with no interference from excipients.

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