PHYTOCHEMICALS: EXTRACTION, ISOLATION & IDENTIFICATION OF BIOACTIVE COMPOUNDS FROM ARISTOLOCHIA BRACTEOLATE

Abstract

Objective:To isolate, analyze and identify the phytochemical compositions from Aristolochia bracteolate leaves. Methods: Preliminary phytochemical analysis for four active constituents, extracted from powdered leaves with different solvents by using Soxhlet apparatus and carried out by different chromatographic techniques. The phytochemical characterizations were evaluated by NMR & MS. Results: This species exhibited & Identified four compounds representing 2.0 % of the total phytochemical composition. The Preliminary phytochemical analysis confirmed the presence of Hydroxystigmast-5-en-7-one, Oleanonic acid, 5,7,4ʹ-trihydroxy-3,8-dimethoxyflavone & 7,9-dimethoxytariacuripyrone. All of them are report for first time from this Spp. Conclusions: From this study, can be concluded that report for the first time from Aristolochia bracteolate leaves, identified 4 pure chemical compounds, by analysis using various physical & spectral techniques.