Abstract:The genus, Hymenocrater, belongs to the plant family Lamiaceae which contains eleven shrub species, of which H. calycinus belonges and grows wildly in Northeastern Iran. From the ethyl acetate and methanol extracts of the flowered aerial parts of Hymenocrater calycinus, four compounds were isolated using chromatographic methods and identified by spectroscopic data (MS, 1 H-and 13 C-NMR, HMQC, HMBC and 1 H-1 H COSY). The effect of rosmarinic acid, as the main component in our study was applied to Staphylococcus… Show more
“…The IR spectrum showed absorption bands at 3165, 1707 and 1617 cm −1 indicating the presence of hydroxyl groups and carboxylic acid function. Its spectral data were in agreement with those previously reported in the literature for the same compound 23, 24, 25, 26. Different spectroscopic data like HMBC, HMQC, and 1 H- 1 H COSY of the isolated compound is presented in Table 3.Fig.…”
BackgroundMedicinal plants constitute a natural reservoir for medicines worldwide. They serve mainstream therapeutics and are central in folklore medicine. In case of Adenium obesum (Lav, Apocynaceae), indigenous people of Oman use it for the treatment of venereal diseases, wounds, skin diseases, headaches, muscle pain as well as joint pain; yet, the active ingredients have not been classified. To screened the antioxidant and antimicrobial activities of an identified compound that we isolated from the highest active chloroform extract.MethodsThe antioxidant and antimicrobial activities of the extracts and the isolated compound were determined by diphenyl-1-picrylhydrazyl (DPPH) and disc diffusion methods. To characterize the compound, we used TLC, column, 1H-NMR, 13C-NMR, 2D-NMR, IR and MS.ResultsThe highest antioxidant activity was found in chloroform extract with EC50 value of 28.32 μg/ml followed by water, methanol, butanol, ethyl acetate and hexane extracts, their IC50 being 29.95, 39.17, 42.40, 43.20 and 57.00 μg/ml respectively. All crude extracts and pure compound displayed moderate antimicrobial activity against one Gram positive Staphylococcus aureus and three Gram negative Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris within growth inhibition range of 0–13 mm. The active metabolite was identified as 3,4-dihydroxycinnamic acid (R)-1-carboxy-2-(3,4-dihydroxyphenyl) ethyl ester which is a common plant ingredient known as rosmarinic acid.ConclusionThe results indicate that walnut chloroform fraction may contain effective compounds with a broad spectrum of curative applications. This is the first report on isolation and characterization of a compound from chloroform crude extract of A. obesum.
“…The IR spectrum showed absorption bands at 3165, 1707 and 1617 cm −1 indicating the presence of hydroxyl groups and carboxylic acid function. Its spectral data were in agreement with those previously reported in the literature for the same compound 23, 24, 25, 26. Different spectroscopic data like HMBC, HMQC, and 1 H- 1 H COSY of the isolated compound is presented in Table 3.Fig.…”
BackgroundMedicinal plants constitute a natural reservoir for medicines worldwide. They serve mainstream therapeutics and are central in folklore medicine. In case of Adenium obesum (Lav, Apocynaceae), indigenous people of Oman use it for the treatment of venereal diseases, wounds, skin diseases, headaches, muscle pain as well as joint pain; yet, the active ingredients have not been classified. To screened the antioxidant and antimicrobial activities of an identified compound that we isolated from the highest active chloroform extract.MethodsThe antioxidant and antimicrobial activities of the extracts and the isolated compound were determined by diphenyl-1-picrylhydrazyl (DPPH) and disc diffusion methods. To characterize the compound, we used TLC, column, 1H-NMR, 13C-NMR, 2D-NMR, IR and MS.ResultsThe highest antioxidant activity was found in chloroform extract with EC50 value of 28.32 μg/ml followed by water, methanol, butanol, ethyl acetate and hexane extracts, their IC50 being 29.95, 39.17, 42.40, 43.20 and 57.00 μg/ml respectively. All crude extracts and pure compound displayed moderate antimicrobial activity against one Gram positive Staphylococcus aureus and three Gram negative Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris within growth inhibition range of 0–13 mm. The active metabolite was identified as 3,4-dihydroxycinnamic acid (R)-1-carboxy-2-(3,4-dihydroxyphenyl) ethyl ester which is a common plant ingredient known as rosmarinic acid.ConclusionThe results indicate that walnut chloroform fraction may contain effective compounds with a broad spectrum of curative applications. This is the first report on isolation and characterization of a compound from chloroform crude extract of A. obesum.
“…The isolated compounds from aerial parts of C. erythraeum were identified as isoquercetin (1), rutin (2), 13,14 along with β-sitosterol (3), [15][16][17] and 2-decenol (4) based on the spectroscopic data ( 1 H-NMR, 13 C-NMR) and compared to the pertinent spectroscopic data in previously published literature (Figure 1). 9 To the best of our knowledge, this is the first report on the isolation and elucidation of secondary metabolites of C. (Table 1).…”
Objective: Cymbocarpum erythraeum (Apiaceae) is an endemic species in Iran. Up to now, there have been no phytochemical and biological investigations on this species. Therefore, isolation of the main secondary metabolites of the plant along with its anti-H. pylori activity have been considered in this paper.
Materials and Methods:The dried parts of the plant were extracted with different solvents using solvent percolation and the antibacterial activity of the extracts evaluated by the disk diffusion method. Four compounds were isolated using different column chromatography methods.
Results:The compounds were identified by proton nuclear magnetic resonance and carbon-13 nuclear magnetic resonance as isoquercetin (1), rutin (2), β-sitosterol (3) and 2-decenol (4).
Conclusion:Anti-H. pylori evaluation of the extracts and isolated compounds against three clinical isolates of H. pylori revealed that hexane extract of the plant inhibited all H. pylori strains. Amaç: Cymbocarpum erythraeum (Apiaceae) İran'a endemik bir türdür. Bu zamana kadar, bu tür üzerinde herhangi bir fitokimyasal ve biyolojik aktivite çalışmasına rastlanmamıştır. Bu nedenle, bu çalışmada, bitkinin ana sekonder metabolitlerinin ve Helicobacter pylori'ye karşı etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Kurutulmuş bitki kısımları perkolasyon yöntemi ile farklı çözücüler kullanılarak ekstre edilmiş ve ekstrelerin antibakteriyel etkisi disk difüzyon yöntemiyle değerlendirilmiştir. Farklı kolon kromatografisi yöntemleri kullanılarak dört bileşik izole edilmiştir. Bulgular: Bileşiklerin yapıları, proton nükleer manyetik rezonans ve karbon-13 nükleer manyetik rezonans tekniği ile izokersetin (1), rutin (2), β-sitosterol (3) ve 2-dekenol (4) olarak belirlenmiştir. Sonuç: Ekstrelerin ve izole edilen bileşiklerin üç H. pylori klinik izolatına karşı etkilerinin değerlendirildiği bu çalışmada, bitkinin hekzan ekstresinin tüm H. pylori suşlarını ihibe ettiği tespit edilmiştir.
“…The 13 C NMR spectral data were compared with rosemeric acid 33 and teucrol. 34 The carbon signals of the sugar unit were comparable to galactoside chain.…”
ABSTRACT:Mentha piperitais currently one of the most economically important aromatic and medicinal crops in all over the world. Phytochemical investigation of the leaves of Mentha piperita (L.) (Lamiaceae) yielded a new compound, which is decarboxyrosemarinic acid galactoside. Formulate as 3', 4'-dihydroxy-β-phenyl ethyl caffeate-4-(3'''-menthyl)-4'-β-D-galacto pyranoside. 13C NMR, 1H NMR, FABMS, IR were used for structural characterization. Wound healing responses of this compound was evaluated from biochemical as well as biophysical parameters and analyze the role of isolated compound. The parameters studied included rate of wound contraction and the period of epithelialization in excision wound model. Tissues were removed on different days of intervals and subsequently analyzed for specific assays Tensile strength in incision wound model was assessed along with histopathological examinations. Compound-1was found to increase the cellular proliferation and collagensynthesis at the wound site, as indicated by increases in amounts of DNA synthesized, protein content. Compound-1treatment was also shown to decrease the levels of lipid peroxides (LPs), while the activity of enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were significantly found to be increased when compared to the control. This study demonstrates and validates the efficacy of Mentha piperita isolated compounds on wound healing activity.
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