Phytochrome A Negatively Regulates the Shade Avoidance Response by Increasing Auxin/Indole Acidic Acid Protein Stability

Yang, Chuanwei; Xie, Famin; Jiang, Ying-Kui; Li, Zhen; Huang, Xu; Li, Lin

doi:10.1016/j.devcel.2017.11.017

Cited by 108 publications

(127 citation statements)

References 57 publications

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“…CRY1 interacts with PIF4 to regulate its transcriptional activity and auxin biosynthesis (Ma et al , ). Furthermore, CRYs and PHYs interact directly with AUX/IAA proteins, stabilizing them and thus inhibiting auxin signaling (Xu et al , ; Yang et al , 2018a). Auxin homeostasis is affected by UV‐B via photooxidative damage, degradation, biosynthesis, and conjugation (Vanhaelewyn et al , ).…”

Section: Discussionmentioning

confidence: 99%

UV‐B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development

et al. 2019

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The UV-B photoreceptor UVR8 mediates multiple UV-B responses in plants, but the function of UVR8 in regulating root development has not previously been investigated. Here, we show that UV-B light inhibits Arabidopsis lateral root growth in a UVR8-dependent manner. Monomeric UVR8 inhibits auxin responses in a tissueautonomous manner and thereby regulates lateral root growth. Genome-wide gene expression analysis demonstrated that auxin and UV-B irradiation antagonistically regulate auxin-regulated gene expression. We further show that UVR8 physically interacts with MYB73/MYB77 (MYB DOMAIN PROTEIN 73/77) in a UV-Bdependent manner. UVR8 inhibits lateral root development via regulation of MYB73/MYB77. When activated by UV-B light, UVR8 localizes to the nucleus and inhibits the DNA-binding activities of MYB73/MYB77 and directly represses the transcription of their target auxin-responsive genes. Our results demonstrate that UV-B light and UVR8 are critical for both shoot morphogenesis and root development. The UV-B-dependent interaction of UVR8 and MYB73/MYB77 serves as an important module that integrates light and auxin signaling and represents a new UVR8 signaling mechanism in plants.

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Section: Discussionmentioning

confidence: 99%

UV‐B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development

et al. 2019

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“…COP1/SPA complex interferes with the interaction of BIN2 and PIF3, thereby repressing the degradation of PIF3 and stabilizing PIF3 (Ling et al 2017). Two recent studies showed that the photoreceptors phyA, phyB, and CRY1 interact directly with and stabilize auxin/indole‐3‐acetic acid (AUX/IAA) proteins to restrict auxin signaling in Arabidopsis (Xu et al 2018; Yang et al 2018a). In addition, recent studies demonstrated that the UV‐B receptor UVR8 and the blue light receptor CRY1 physically interact with the BIM1 and BES1 TFs to inhibit BR signaling through interfering with the DNA‐binding activity of BES1 (Liang et al 2018; Wang et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Photoexcited phytochrome B interacts with brassinazole resistant 1 to repress brassinosteroid signaling in Arabidopsis

Dong

Liu

et al. 2019

JIPB

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Photoreceptor phytochrome B (phyB) mediates a variety of light responses in plants. To further elucidate the molecular mechanisms of phyB-regulated hypocotyl elongation, we performed firefly luciferase complementation imaging (LCI) screening for phyB-interacting transcription factors (TFs). LCI assays showed that phyB possibly interacts with brassinazoleresistant 1 (BZR1), BZR2, AUXIN RESPONSE FACTOR 6 (ARF6), and several WRKY DNA-binding TFs in a red light-dependent manner. Furthermore, biochemical assays demonstrated that photoexcited phyB specifically interacts with nonphosphorylated BZR1, the physiologically active form of a master TF in brassinosteroid (BR) signaling, and this interaction can be competitively interfered by phytochrome-interacting factor 4. Furthermore, we showed that phyB can directly interact with the DNA-binding domain of BZR1 and affect the enrichment of BZR1 on the chromatin of target genes. Moreover, our genetic evidence and RNA-seq analysis demonstrated that phyB negatively regulates BR signaling. Together, we revealed that photoexcited phyB directly interacts with the TF BZR1 to repress BR signaling in Arabidopsis.

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“…In Arabidopsis thaliana, there are five distinct phytochromes, phyA to phyE; phyA is a type I phytochrome and is the most abundant phytochrome species in etiolated seedlings, whereas phyB to phyE are all type II phytochromes, and phyB is the most abundant phytochrome in light-grown plants (1)(2)(3). PhyA is the only photoreceptor in plants responsible for initiating responses in FR light, and thus is unique to higher plants enabling them to de-etiolate in shady conditions characterized by a high FR content (1,2,4,5). Both type I and type II phytochrome molecules can be divided into an N-terminal domain (∼70 kDa) and a C-terminal domain (∼55 kDa), which are connected by a flexible hinge region (2,6).…”

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confidence: 99%

Hinge region of Arabidopsis phyA plays an important role in regulating phyA function

Zhou

Yang

Duan

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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Phytochrome A (phyA) is the only plant photoreceptor that perceives far-red light and then mediates various responses to this signal. Phosphorylation and dephosphorylation of oat phyA have been extensively studied, and it was shown that phosphorylation of a serine residue in the hinge region of oat phyA could regulate the interaction of phyA with its signal transducers. However, little is known about the role of the hinge region of Arabidopsis phyA. Here, we report that three sites in the hinge region of Arabidopsis phyA (i.e., S590, T593, and S602) are essential in regulating phyA function. Mutating all three of these sites to either alanines or aspartic acids impaired phyA function, changed the interactions of mutant phyA with FHY1 and FHL, and delayed the degradation of mutant phyA upon light exposure. Moreover, the in vivo formation of a phosphorylated phyA form was greatly affected by these mutations, while our data indicated that the abundance of this phosphorylated phyA form correlated well with the extent of phyA function, thus suggesting a pivotal role of the phosphorylated phyA in inducing the far-red light response. Taking these data together, our study reveals the important role of the hinge region of Arabidopsis phyA in regulating phyA phosphorylation and function, thus linking specific residues in the hinge region to the regulatory mechanisms of phyA phosphorylation.Arabidopsis | far-red light | phyA | hinge region | phosphorylation

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Phytochrome A Negatively Regulates the Shade Avoidance Response by Increasing Auxin/Indole Acidic Acid Protein Stability

Cited by 108 publications

References 57 publications

UV‐B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development

UV‐B photoreceptor UVR8 interacts with MYB73/MYB77 to regulate auxin responses and lateral root development

Photoexcited phytochrome B interacts with brassinazole resistant 1 to repress brassinosteroid signaling in Arabidopsis

Hinge region of Arabidopsis phyA plays an important role in regulating phyA function

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