Phytoestrogen (+)-pinoresinol exerts antitumor activity in breast cancer cells with different oestrogen receptor statuses

Abstract

BackgroundConsumption of virgin olive oil (VOO) has been associated with a low breast cancer incidence. Pinoresinol is a phytoestrogen that is typically found in VOO. Considering the role of oestrogen in breast cancer development and progression, we investigated the potential antitumor activity of pinoresinol in breast cancer cells.MethodsTo address this question, we treated MDA-MB-231 (oestrogen receptor [ER] negative) and MCF7 (ER+) human breast tumour cells and MCF10A human mammary epithelial cells (ER-) with different concentrations of pinoresinol. The cytotoxic activity, cell proliferation, cell cycle profile, apoptosis induction, reactive oxygen species production and DNA damage were assessed.ResultsPinoresinol showed cytotoxic, anti-proliferative and pro-oxidant activity in human breast tumour cells, independent of their oestrogen receptor status. In addition, pinoresinol exerted antioxidant activity and prevented DNA damage associated with oxidative stress in human mammary epithelial cells.ConclusionsOverall, the results suggest that pinoresinol may have antitumor activity in human breast cancer cells independently of oestrogen receptor status. Furthermore, the results show that the pinoresinol has the typical characteristics of a chemopreventive compound.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1233-7) contains supplementary material, which is available to authorized users.

Withdrawn: The Structural Bioinformatics Analysis of Biophenolic Lignan- Estrogen Receptor Interaction

“…More than half of all breast cancers over express ER and approximately 70% of them respond to anti-estrogen (for instance tamoxifen) therapy [1]. Drug resistance issues have brought about the search for and the development of various hormonal therapies designed to inhibit ER action, while research on the mechanisms underlying resistance has cast light on the cellular mechanisms besides ligand binding, which control ER function [23][24].…”

Change in quality during ripening of olive fruits and related oils extracted from three minor autochthonous Sardinian cultivars

“…An important contribute to the polyphenolic fraction was also exerted by pinoresinol that was the second most abundant polyphenol in CO and was not detected by Deiana et al (2019). Pinoresinol has been recently addressed to potentially have antitumor activity in human breast cancer cells (López-Biedma et al, 2016), although it has recently been demonstrated that pinoresinol is responsible for decreasing vitamin D intestinal absorption in rats (Goncalves et al, 2018). Phenolic acids and alcohols were detected in low amounts, while a good content of the flavonoids luteolin and apigenin, which was comparable to that reported by Deiana et al (2019), was detected.…”

Do Olive and Fish Oils of the Mediterranean Diet Have a Role in Triple Negative Breast Cancer Prevention and Therapy? An Exploration of Evidence in Cells and Animal Models

“…All searches returned a combined total of 50 studies published within our selected time frame (5 y). After filtering for relevance, we identified a total of 34 articles reviewed below ( 41 – 74 ). Some studies that were published outside our selected time frame (>5 y) were identified in post-hoc searches and also reviewed to enhance the perspective and provide additional commentary.…”

“…Previous studies had demonstrated pinoresinol (100 μM) had anti-proliferative effects on MDA-MB-231 cells (70 ± 6% cell survival) but not MCF7 cells (96 ± 7% cell survival) ( 126 ). Recently, pinoresinol treatment was shown to have cytotoxic effects on MDA-MB-231 cells at concentrations ranging from 0.001–100 μM, whereas only low levels (0.001–0.01 μM) were cytotoxic to MCF7 cells ( 74 ). Interestingly, the cytotoxic effect of pinoresinol may be specific to tumorigenic cells.…”