Phytogenic synthesis of silver nanobactericides for anti-biofilm activity against human pathogen H. pylori

Abstract

The present study reports the phytogenic synthesis of silver nanobactericides using Acorus calamus L. and their antibiofilm activity against clinically isolated H. pylori. The synthesis was confirmed with change in the color of the reaction mixture to brown. The increased in the color intensity was periodically monitored with UV-visible spectroscopy which displayed maximum absorption at 410 nm. The biomolecular interaction was studied with FTIR spectral measurements of silver nanobactericides which revealed the presence of broad absorbance band appearing at 3361 is due to OH group and the prominent peak at 1634 correspond to an amide group. X-ray diffraction (XRD) displayed Bragg's intensities at 2θ angle reflecting (111), (200), (220) and (311) of the face centered cubic (fcc) structure of silver which was compared with standard XRD pattern. The morphological characteristics of nanobactericides were studied using Transmission electron microscopy (TEM) analysis which revealed the polydispersity of nanoparticles with size ranging from 5 to 60 nm. The anti-biofilm activity of silver nanobactericides against H. pylori was measured using crystal violet and ruthenium red assays which revealed 350 µg/mL to be more effective. The obtained activity was validated with standard antibiotics amoxicillin. Overall, the results obtained in the present investigation are promising enough to reveal the efficacy of silver nanoparticles to inhibit the biofilm production.

Phyto-fabricated Nanoparticles and Their Anti-biofilm Activity: Progress and Current Status

“…The biofilm inhibitory activity was evaluated against Helicobacter pylori clinical isolates. The synthesized NPs exhibited significant antibiofilm activity at concentration 350 μg/ml, as evidenced by crystal violet and ruthenium red assays (Prasad et al, 2019).…”

Helicobacter Pylori-Induced Gastric Infections: From Pathogenesis to Novel Therapeutic Approaches Using Silver Nanoparticles

“…Non-spherical silver ultrananoclusters, with the smallest size of all the AgNPs, were used in a single study [ 124 ], and they showed a high degree of growth inhibition of the bacterium when the dosage was between 0.16 and 0.33 mg/L. In the vast majority of the studies, spherical silver nanoparticles were used, obtained from different extracts, having various sizes: A. priceps , 5–30 nm [ 125 ]; S. xanthocarpum , 4–18 nm [ 32 , 126 ]; T. vernicifluum , 4–20 nm [ 127 ]; C. longa [ 128 ] and A. nilotica , 22–66 nm [ 124 ]; P. putida , 6–16 nm [ 35 ]; A. calamus , 5–60 nm [ 129 ]; A. nilotica , 22–55 nm [ 130 ]. The most common effect on the bacterial colonies was inhibition of growth, which was presented in six of the papers [ 124 , 125 , 127 , 128 , 131 , 132 , 133 , 134 ], followed by activation of reactive oxygen species production [ 130 , 132 ], inactivation of H. pylori urease [ 131 , 135 ], bacterial DNA fragmentation and consecutive loss of viability [ 130 ], damage to the bacterial cellular membrane [ 132 ], and activation of antioxidant activity [ 136 ].…”