Phytohormone profiles and related gene expressions after endodormancy release in developing Pinus tabuliformis male strobili

Zhang, Jingxing; Li, Hongmei; Yang, Boning; Wang, Huili; Niu, Shihui; El‐Kassaby, Yousry A.; Li, Wei

doi:10.1016/j.plantsci.2021.111167

Cited by 8 publications

(6 citation statements)

References 54 publications

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“…Moreover, the expression level of these genes was increased in these drought stress situations (Rasheed et al, 2018). ALDH2B4 is an enzyme involved in IAA synthesis (Hou et al, 2020;Zhang et al, 2022). It is possible that the observed increase in the expression of this enzyme is related to the increase in IAA detected by GC-MS.…”

Section: Role Of Aldhs Tc-induced Metabolic Alterations In Arabidopsi...mentioning

confidence: 95%

Trans-cinnamaldehyde-related overproduction of benzoic acid and oxidative stress on Arabidopsis thaliana

López-González

Ferradás

Araniti³

et al. 2023

Front. Plant Sci.

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IntroductionTrans-cinnamaldehyde is a specialised metabolite that naturally occurs in plants of the Lauraceae family. This study focused on the phytotoxic effects of this compound on the morphology and metabolism of Arabidopsis thaliana seedlings.Material and methodsTo evaluate the phytotoxicity of trans-cinnamaldehyde, a dose-response curve was first performed for the root growth process in order to calculate the reference inhibitory concentrations IC50 and IC80 (trans-cinnamaldehyde concentrations inducing a 50% and 80% inhibition, respectively). Subsequently, the structure and ultrastructure of the roots treated with the compound were analysed by light and electron microscopy. Based on these results, the following assays were carried out to in depth study the possible mode of action of the compound: antiauxinic PCIB reversion bioassay, determination of mitochondrial membrane potential, ROS detection, lipid peroxidation content, hormone quantification, in silico studies and gene expression of ALDH enzymes.ResultsTrans-cinnamaldehyde IC50 and IC80 values were as low as 46 and 87 μM, reducing the root growth and inducing the occurrence of adventitious roots. At the ultrastructural level, the compound caused alterations to the mitochondria, which were confirmed by detection of the mitochondrial membrane potential. The morphology observed after the treatment (i.e., appearance of adventitious roots) suggested a possible hormonal mismatch at the auxin level, which was confirmed after PCIB bioassay and hormone quantification by GC-MS. The addition of the compound caused an increase in benzoic, salicylic and indoleacetic acid content, which was related to the increased gene expression of the aldehyde dehydrogenase enzymes that can drive the conversion of trans-cinnamaldehyde to cinnamic acid. Also, an increase of ROS was also observed in treated roots. The enzyme-compound interaction was shown to be stable over time by docking and molecular dynamics assays.DiscussionThe aldehyde dehydrogenases could drive the conversion of trans-cinnamaldehyde to cinnamic acid, increasing the levels of benzoic, salicylic and indoleacetic acids and causing the oxidative stress symptoms observed in the treated seedlings. This would result into growth and development inhibition of the trans-cinnamaldehyde-treated seedlings and ultimately in their programmed-cell-death.

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Section: Role Of Aldhs Tc-induced Metabolic Alterations In Arabidopsi...mentioning

confidence: 95%

Trans-cinnamaldehyde-related overproduction of benzoic acid and oxidative stress on Arabidopsis thaliana

López-González

Ferradás

Araniti³

et al. 2023

Front. Plant Sci.

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“…GA has critical interaction with auxin through induction of PIN1, leading to the stimulation of cambium proliferation [45]. GAs have been reported to be key signals for the release of dormancy in gymnosperm seeds and buds [46][47][48]. In Manchurian red pine (Pinus tabuliformis), GAs are induced in the early spring to break bud dormancy by blocking DELLA, resulting in expression of FTL2 (Flowering Locus T/Terminal Flower1-like 2) that then induces flowering [48].…”

Section: Hormonal Regulation Of Seasonal Wood Formationmentioning

confidence: 99%

“…GAs have been reported to be key signals for the release of dormancy in gymnosperm seeds and buds [46][47][48]. In Manchurian red pine (Pinus tabuliformis), GAs are induced in the early spring to break bud dormancy by blocking DELLA, resulting in expression of FTL2 (Flowering Locus T/Terminal Flower1-like 2) that then induces flowering [48]. The induced expression of GA20-oxidase1 and GA20-oxidase2 genes increased GA concentrations in Ginkgo (Ginkgo biloba) embryos upon the dormancy release of seeds [47].…”

Section: Hormonal Regulation Of Seasonal Wood Formationmentioning

confidence: 99%

Exploring the Seasonal Dynamics and Molecular Mechanism of Wood Formation in Gymnosperm Trees

Nguyen

Bae

Tran

et al. 2023

IJMS

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Forests, comprising 31% of the Earth’s surface, play pivotal roles in regulating the carbon, water, and energy cycles. Despite being far less diverse than angiosperms, gymnosperms account for over 50% of the global woody biomass production. To sustain growth and development, gymnosperms have evolved the capacity to sense and respond to cyclical environmental signals, such as changes in photoperiod and seasonal temperature, which initiate growth (spring and summer) and dormancy (fall and winter). Cambium, the lateral meristem responsible for wood formation, is reactivated through a complex interplay among hormonal, genetic, and epigenetic factors. Temperature signals perceived in early spring induce the synthesis of several phytohormones, including auxins, cytokinins, and gibberellins, which in turn reactivate cambium cells. Additionally, microRNA-mediated genetic and epigenetic pathways modulate cambial function. As a result, the cambium becomes active during the summer, resulting in active secondary xylem (i.e., wood) production, and starts to become inactive in autumn. This review summarizes and discusses recent findings regarding the climatic, hormonal, genetic, and epigenetic regulation of wood formation in gymnosperm trees (i.e., conifers) in response to seasonal changes.

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“…However, suitable genetic markers for seed orchard studies of this species are scarce. Previous studies have focused on its growth and development [2][3][4], drought stress response [5], and genetic diversity [6].…”

Section: Introductionmentioning

confidence: 99%

“…Guo et al [4] performed transcriptomic and proteomic analyses of the far-red light effect on stem elongation in the presence or absence of PAC and investigated the molecular mechanism of FR-regulated stem elongation under PAC treatment. Zhang et al [3] studied the plant hormone spectrum and related gene expression after megagametophyte release in developing male cones and analyzed the endogenous hormones and their related genes, verifying the important role of abscisic acid (ABA) in plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances

Wang,

Guo,

Zhang

et al. 2023

Forests

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Chinese pine (Pinus tabuliformis) is a conifer species endemic to the temperate, warm temperate, and semi-arid regions of China. It has important ecological and economic values, but lacks suitable molecular markers for genetic studies. In this study, we collected open-pollinated progeny seeds from four provenances: Ningcheng (Nc), Qinyuan (Qy), Weichang (Wc), and Pingquan (Pq). We sequenced the transcriptomes of open-pollinated progeny seedlings from four provenances and annotated 18,244 unigenes. We analyzed the expression of genes involved in the auxin indole-3-acetic acid (IAA), cytokinin (CTK), and gibberellin (GA) signaling pathways among the provenances. Additionally, we detected 2811 expressed sequence tag simple sequence repeat (EST-SSR) loci in 2360 unigenes, with a frequency of 14.83% and an average of one locus per 14,556 base pairs (bp). We developed 10 polymorphic primers from 67 pairs and tested them on 56 samples from the four provenances. These primers exhibited moderate to high polymorphism and distinguished all samples clearly. Our study reveals variation in growth and development among open-pollinated progeny seedlings from different provenances of Chinese pine and provides novel markers for its genetic diversity study and marker-assisted breeding.

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Phytohormone profiles and related gene expressions after endodormancy release in developing Pinus tabuliformis male strobili

Cited by 8 publications

References 54 publications

Trans-cinnamaldehyde-related overproduction of benzoic acid and oxidative stress on Arabidopsis thaliana

Trans-cinnamaldehyde-related overproduction of benzoic acid and oxidative stress on Arabidopsis thaliana

Exploring the Seasonal Dynamics and Molecular Mechanism of Wood Formation in Gymnosperm Trees

Transcriptome Analysis and Novel EST-SSR Marker Development for Pinus tabuliformis Seedlings from Four Provenances

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