Phytomedicinal Role of Pithecellobium dulce against CCl₄‐mediated Hepatic Oxidative Impairments and Necrotic Cell Death

Abstract

Present study investigates the beneficial role of the aqueous extract of the fruits of Pithecellobium dulce (AEPD) against carbon tetrachloride (CCl4)-induced hepatic injury using a murine model. AEPD has been found to possess free radical (DPPH, hydroxyl and superoxide) scavenging activity in cell-free system. CCl4 exposure increased the activities of various serum maker enzymes and intracellular reactive oxygen species (ROS) production. In line with these findings, we also observed that CCl4 intoxication increased the lipid peroxidation and protein carbonylation accompanied by decreased intracellular antioxidant defense, activity of cytochrome P450 and CYP2E1 expression. DNA fragmentation and flow cytometric analyses revealed that CCl4 exposure caused hepatic cell death mainly via the necrotic pathway. Treatment with AEPD both pre- and post-toxin exposure protected the organ from CCl4-induced hepatic damage. Histological findings also support our results. A well-known antioxidant vitamin C was included in this study to compare the antioxidant potency of AEPD. Combining all, results suggest that AEPD protects murine liver against CCl4-induced oxidative impairments probably via its antioxidative property.

Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

“…Becker et al [37] stated that elevated activities of these enzymes are indicative of cellular leakage and loss of the functional integrity of liver cell membrane. The elevation of plasma enzymes concentration is generally regarded as one of the sensitive markers of hepatic damage [38]. In our study, CCl 4 was found to cause significant increases in plasma AST, ALT and GGT levels compared to control (p < 0.001).…”

A probable crosstalk between Ca+2, reactive oxygen species accumulation and scavenging mechanisms and modulation of protein kinase C activity during seed development in sunflower

“…30 Tissue homogenates were filtered through 4 layers of muslin cloth and centrifuged at 10 000 g for 20 min at 4 °C. ROS was estimated using de-esterified 2,7-dichlorofluorescein (DCFH) obtained from DCFH-DA by the hydrolysis in NaOH.…”

“…14 Present investigations aim at understanding the possible correlation among the various phases of seed development, generation, and enzymatic scavenging of ROS during these phases, activation of protein kinase C, and its modulation by calcium. In this context, qualitative and quantitative analysis of major ROS scavenging enzymes, namely superoxide dismutase (SOD), peroxidase (POD), and glutathione reductase (GR), has been undertaken at 3 stages of sunflower seed development (20,30, and 40 d after anthesis). Modulation of the activity of these enzymes with seed development has been examined for a possible correlation with calcium-stimulated protein kinase C (PKC) activity.…”

Tribal Way of Livestock Husbandry: An Ethnobotanical Survey of Purulia District, India in Search for Fodder Plants: Neutraceutical and Pharmaceutical Relevance

“…Benth. Chaudhary and Taparia (2007) Hepatoprotective (Manna et al, 2011(Manna et al, ) (1990 and Paengkoum and Paengkoum (2010) Pterocarpus marsupium Roxb. Ally and Kunjikutty (2003) Ally and Kunjikutty Hepatoprotective (Devipriya et al, 2007), (2003) hypoglycaemic (Dhanabal et al, 2006) Schleichera oleosa Prasad et al (1991) and Makkar et al (1990) Cytotoxic, radical-scavenging (Lour.)…”