Phytophthora Species, New Threats to the Plant Health in Korea

Hyun, Ik-Hwa; Choi, Won San

doi:10.5423/ppj.rw.07.2014.0068

Cited by 17 publications

(8 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Projections include most of mainland China, Korea, southern Sweden, Ethiopia, Laos, Thailand and Mynmar. Phytophthora cinnamomi has been detected in South Korean forests (Hyun & Choi, ), data missing from the CABI/EPPO map (Anonymous, ). While earlier surveys of oaks in southern Sweden have not recovered P. cinnamomi (Jönsson et al ., ), if introduced, our model predicts that P. cinnamomi could persist.…”

Section: Discussionmentioning

confidence: 99%

Current and projected global distribution of Phytophthora cinnamomi, one of the world's worst plant pathogens

Burgess

Scott

McDougall

et al. 2016

Global Change Biology

211

170

View full text Add to dashboard Cite

Accepted ArticleThis article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/gcb.13492 This article is protected by copyright. All rights reserved. Accepted ArticleThis article is protected by copyright. All rights reserved. AbstractGlobally, Phytophthora cinnamomi is listed as one of the 100 worst invasive alien species and active management is required to reduce impact and prevent spread in both horticulture and natural ecosystems. Conversely, there are regions thought to be suitable for the pathogen where no disease is observed. We developed a CLIMEX model for the global distribution of P. cinnamomi based on the pathogen's response to temperature and moisture and by incorporating extensive empirical evidence on the presence and absence of the pathogen. The CLIMEX model captured areas of climatic suitability where P. cinnamomi occurs that is congruent with all available records. The model was validated by the collection of soil samples from asymptomatic vegetation in areas projected to be suitable by the model for which there were few records. DNA was extracted and the presence or absence of P. cinnamomi determined by high throughput sequencing (HTS). While not detected using traditional isolation methods, HTS detected P. cinnamomi at higher elevations in eastern Australia and central Tasmania as projected by the CLIMEX model. Further support for the CLIMEX model was obtained by using the large dataset from southwest Australia where the proportion of positive records in an area is related to the Ecoclimatic Index value for the same area. We provide for the first time a comprehensive global map of the current P. cinnamomi distribution, an improved CLIMEX model of the distribution, and a projection to 2080 of the distribution with predicted climate change. This information provides the basis for more detailed regional scale modelling and supports risk assessment for governments to plan management of this important soil-borne plant pathogen.

show abstract

Section: Discussionmentioning

confidence: 99%

Current and projected global distribution of Phytophthora cinnamomi, one of the world's worst plant pathogens

Burgess

Scott

McDougall

et al. 2016

Global Change Biology

211

170

View full text Add to dashboard Cite

show abstract

“…Most Phytophthora species can cause serious diseases in a diverse range of crops and plants under ideal environmental conditions. 1 Among the top 10 oomycetes in molecular plant pathology, there are 6 Phytophthora species, including P. capsici. 2 P. capsici has a wide range of hosts, and can infect 51 genera in 28 families (http://idtools.org/id/ phytophthora/factsheet.php?name=7926).…”

Section: ■ Introductionmentioning

confidence: 99%

Activity and Resistance-Related Point Mutations in Target Protein PcORP1 of Fluoxapiprolin in Phytophthora capsici

Miao

Liu

et al. 2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

Fluoxapiprolin is a new piperidinyl thiazole isoxazoline fungicide developed by Bayer Crop Science in 2012, but the sensitivity and resistance mechanism of fluoxapiprolin are unclear. In this study, the sensitivities of 130 Phytophthora capsici isolates to fluoxapiprolin were determined, and a unimodal distribution was observed with a mean EC50 value of 0.00043 μg/mL. Nine stable fluoxapiprolin-resistant mutants were obtained by fungicide adaption. The fitness of mutants was similar to or lower than that of the corresponding parental isolate. Seven heterozygous point mutations in the target protein PcORP1 were found in these mutants. These point mutations were confirmed in PsORP1 of P. sojae homologue positions using the CRISPR/Cas9 system. G770V and N835S+I877F do confer high fluoxapiprolin resistance (resistance factor, RF > 1000), and ΔN835, N767I, and N837T+S910C lead to low resistance (RF < 100). P. sojae transformants containing L733W, S768F, S768Y, ΔG818/F819, N837Y, N837F, P861H, L863W, and I877Y in PsORP1 were also associated with high fluoxapiprolin resistance (RF > 100). In conclusion, the resistance risk of P. capsici to fluoxapiprolin is moderate, and multiple point mutations in PcORP1 could confer different resistance levels to fluoxapiprolin.

show abstract

“…Understanding Avr proteins and their role in plant pathology is of key interest to several economic sectors, and includes national defense [10,67]. Understanding these proteins at the molecular level can provide the foundation needed to accomplish desired goals of all these areas.…”

Section: Discussionmentioning

confidence: 99%

Molecular Dynamics Simulations of Phytopthora sojae Avirulence Factor 5, PsAvh5, Define Membrane Binding, Inositolphosphate-3’-phosphate Interactions and Protein Mechanics

2021

Preprint

View full text Add to dashboard Cite

Phytopthora Avirulence proteins are a primary target for development of rational chemical and biological control of some of the most devastating plant pathogens. Despite the sequencing of entire genomes, and characterization of many of these proteins at the chemical level, many questions remain regarding actual chemical and biological interactions involved. In addition, disputed roles of ligands, such as Inositolphosphate-3’-phosphate and amino acids of important function remain unclear. To address some of these issues, we developed molecular models from structural elements and published data for Phytopthora sojae avirulence protein 5. Molecular dynamics simulations are used to study protein function, interactions involved primarily with lipids and membranes, and inositol derivatives. Our findings indicate that the protein is stable as a monomer, and in a dimeric form. Also, that these proteins interact with Inositolphosphate-3’phosphate as a necessary membrane element, in binding. We identified several amino acids of importance, additional to defining the mechanical features of the protein within the binding process to different membranes. A high affinity, comparable to other membrane surface binding molecules of −219.54 Kcal for the dimer, and −176.61 for the monomer were determined. With either form, we found the inositolphosphate-3’-phosphate to be essential in the membrane binding process. Our findings answer some of the debated questions while creating a point to further test avirulence proteins in general for functional aspects. Additionally, the structures and data can be utilized to provide a better starting point for rational design approaches to control this pathogen.

show abstract

Phytophthora Species, New Threats to the Plant Health in Korea

Cited by 17 publications

References 57 publications

Current and projected global distribution of Phytophthora cinnamomi, one of the world's worst plant pathogens

Current and projected global distribution of Phytophthora cinnamomi, one of the world's worst plant pathogens

Activity and Resistance-Related Point Mutations in Target Protein PcORP1 of Fluoxapiprolin in Phytophthora capsici

Molecular Dynamics Simulations of Phytopthora sojae Avirulence Factor 5, PsAvh5, Define Membrane Binding, Inositolphosphate-3’-phosphate Interactions and Protein Mechanics

Contact Info

Product

Resources

About