Based on the materials of studies of phytoplankton of the tributaries of the Ob (Tom’, Ket’, Vasyugan) in the summer of 2019, the species composition, taxonomic structure, abundance and biomass of phytoplankton were established, the water quality class and trophic status were determined. The basis of the species richness of phytoplankton in the tributaries is created by green algae (Chlorophyta), the parameters of alpha diversity indicate the average complexity of the structure of phytoplanktocenosis. In terms of phytoplankton biomass, the trophic status of the rivers varied from mesotrophic to eutrophic category, the water quality class corresponded to class 3 “satisfactory purity”.