Water contamination by heavy metals is an environmental threat, endangering human health. Phytoremediation, through artificial wetlands, emerges as an eco-friendly method for heavy metals decontamination. This study aimed to assess the efficiency of Phalaris arundinacea L. (canary seed plant) in removal of arsenic and manganese from a saturated sand culture under different hydraulic retention times (HRT). The research consisted of two distinct experiments utilizing a completely randomized factorial design, encompassing three factors: two bed lengths (BL) of 50 and 100 cm, four inflow concentrations (Ci) of heavy metals (arsenic at 0, 2, 4, and 6 mg/L, and manganese at 0, 10, 20, and 30 mg/L), and five HRT (3, 6, 9, 12 and 15 days), in three replications. Results revealed that the maximum arsenic absorption (Absmax) (5.88 mg/L) occurred at Ci of 6 mg/L, HRT of 6 days and BL of 50 cm. However, the manganese Absmax (29.25 mg/L) was observed at Ci of 30 mg/L, HRT of 9 days and BL of 50 cm. Phalaris bio-concentration factors (BCF) were 2.29 and 0.15 for arsenic and manganese, respectively, highlighting superior arsenic absorption by 15.26 times. The surface absorption values of arsenic and manganese in the sand bed (qeAs=0.005 mg/g and qeMn=0.004 mg/g) confirmed the insignificant effect of this process in this experiment. The average maximum removal efficiency (RE) for manganese (99.61 %) attained at Ci of 20 mg/L, BL of 100 cm, on the fifteenth day, however that of arsenic (98.11 %) reached at Ci of 6 mg/L, BL of 50 cm, on the sixth day. These findings underscore the Phalaris exceptional capacity to efficiently remove dissolved manganese and arsenic under saturated conditions in the sand culture.