Phytoremediation of cadmium-polluted soil assisted by D-gluconate-enhanced Enterobacter cloacae colonization in the Solanum nigrum L. rhizosphere

Xu, Zhiguang; Wang, Dongsheng; Wanpeng, Tang; Wang, Lili; Li, Qu-Sheng; Lu, Ziyan; Liu, Hui; Zhong, Yuming; He, Tao; Guo, Song

doi:10.1016/j.scitotenv.2020.139265

Cited by 65 publications

(14 citation statements)

References 78 publications

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“…Heavy metal pollution in soil is one of the most important factors restricting agriculture development and endangering human health. Hyperaccumulators, such as sedum, solanum, rapeseed, mustard, clover, switchgrass, were used to adsorb or degrade heavy metal pollutants in the soil, which as an ideal method at present being the characteristics of simple, low cost, little damage to farmland, suitable for large-area treatment and not easy to cause secondary pollution ( Yu et al., 2019 ; Nazli et al., 2020 ; Xu et al., 2020 ). However, compared with the super-accumulating bacteria, cotton is an inedible cash crop with a large biomass and a strong ability to absorb and accumulate Cd ( Li et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton

Yan²,

Li³

et al. 2022

Front. Plant Sci.

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Cadmium (Cd), a non-essential element for plant, is a ubiquitous and highly toxic heavy metal, seriously endangering agricultural production and human health. As a nonedible economic crop, cotton (Gossypium hirsutum L.) has great potential in remediation of Cd contaminated soil, but its underlying mechanism is still unknown. Melatonin (MT), as a plant growth regulator, is involved in alleviating Cd toxicity in some plants, but the molecular mechanisms of MT-mediated Cd detoxification in cotton are largely unknown. This study investigated the possible molecular mechanisms of the MT-mediated Cd detoxification in cotton seedlings by comparative transcriptomic and metabolomic analyses. The results showed that the cotton seedlings were dwarfed and the leaves were wilted and yellow under Cd stress. The application of 50 µmol L−1 MT significantly increased the superoxide dismutase (SOD) activity and malondialdehyde (MDA) content under Cd stress, but 100 µmol L−1 MT significantly decreased SOD activity, while increased ascorbate peroxidase (APX) activity significantly. The addition of 100 μmol L−1 MT significantly increased Cd concentration in the shoots and roots under Cd stress. RNA-seq analysis showed that 5573, 7105, 7253, 25, 198, 9 up-regulated and 6644, 7192, 7404, 9, 59, 0 down-regulated differentially expressed genes (DEGs) were identified in the comparisons of CK vs T1, CK vs T2, CK vs T3, T1 vs T2, T1 vs T3 and T2 vs T3, respectively. It was revealed that MT promoted the expression of certain related genes under Cd stress, and the effect of 100 µmol L−1 MT was better. Moreover, UPLC-MS/MS widely targeted metabolites analyses showed that 195, 150, 150, 12, 24, 59 up-regulated and 16, 11, 23, 38, 127, 66 down-regulated differentially accumulated metabolites (DAMs) were changed in the CK vs T1, CK vs T2, CK vs T3, T1 vs T2, T1 vs T3 and T2 vs T3, respectively. It was revealed that MT induced the synthesis of alkaloids and flavonoids, and inhibited or reduced the synthesis of lipids, amino acids and their derivatives. The comprehensive analyses of transcriptomic and metabolic data showed that 33 DEGs and 4 DAMs, 46 DEGs and 16 DAMs, and 1 DEGs and 1 DAMs were dominantly involved in the pathways of valine, leucine and isoleucine degradation, ABC transporter, alpha-linolenic acid metabolism, respectively. It was revealed that there were three major mechanisms involved in MT-mediated Cd detoxification in cotton, including the enhancement of antioxidant capacity regulated by APX, flavonoids and alkaloids; accumulation of secondary metabolites related to Cd chelation, such as amino acids and derivatives; and regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the MT-mediated Cd stress response.

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Section: Discussionmentioning

confidence: 99%

Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton

Yan²,

Li³

et al. 2022

Front. Plant Sci.

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“…The purity of DNA was checked by NanoDrop spectrophotometer ND-2000c (Thermo Fisher Scientific, United States). The V3-V4 regions of 16S rRNA were amplified with the primers 338F and 806R to demonstrate the composition and structure of the bacterial community ( Xu et al, 2020 ). About 20 μl of the reaction system was applied, including 4 μL of × 5 FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of forward primer, 0.8 μL of reverse primer, 0.4 μL of FastPfu polymerase, 0.2 μL of BSA, 10 ng of DNA template, and ddH 2 O.…”

Section: Methodsmentioning

confidence: 99%

Effect of soil management systems on the rhizosphere bacterial community structure of tobacco: Continuous cropping vs. paddy-upland rotation

et al. 2022

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Rhizosphere bacteria play important role in soil nutrient cycling and plant growth, and their richness and diversity are influenced by soil management systems. However, the specific changes in tobacco rhizosphere bacterial community structure in continuous and tobacco-rice rotation cropping systems remain uninvestigated. In this study, soil properties and the composition of the rhizosphere bacterial community in tobacco monocropping and tobacco-rice rotation cropping systems were analyzed. Moreover, the comparison of rhizosphere bacterial community structure between tobacco continuous and tobacco-rice rotation cropping systems was performed via high-throughput sequencing. The changes in the composition of the rhizosphere bacterial community were investigated at different tobacco growth stages. The results showed that continuous tobacco cropping increased the soil soluble organic carbon (SOC), total nitrogen (TN), and the content of other nutrients (e.g., available phosphorus and available potassium) compared to tobacco-rice rotation cropping. However, monocropping decreased bacterial alpha-diversity and altered the community composition when compared to the rotation cropping system. At the phylum level, the relative abundance of Proteobacteria, Gemmatimonadetes, and Bacteroidetes increased in the continuous cropping soil, while that of Acidobacteria, Firmicutes, and Actinobacteria decreased. At the genera level, the average abundance of the dominant genus Bacillus varied from 12.96% in continuous cropping libraries to 6.33% in the rotation cropping libraries (p < 0.05). Additionally, several other taxa, such as o_Acidobacteriales and Candidatus_Solibacter decreased from 7.63 to 6.62% (p < 0.05) and 4.52 to 2.91% (p < 0.05), respectively. However, the relative abundance of f_Gemmatimonadaceae and c_Subgroup_6 showed an increase of 1.46% (p < 0.05) and 1.63% (p < 0.05) in the tobacco-rice rotation cropping system, respectively. The results of NMDS indicated that the rhizobacteria community structure differed in the two cropping systems. In tobacco, the rhizosphere bacterial community structure showed no significant changes in the prosperous long-term stage and topping stage, but the composition changed significantly in the mature stage.

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“…6E–H), further showing the light‐mediated impacts on bacterial growth and organic acid metabolism via this photoreceptor (CmoBphp). Previous work demonstrated that microorganisms can utilize organic acids such as pyruvate and propionic acid for growth via regulation of the TCA cycle for generation of energy and reduction equivalents (Veit et al ., 2009; Matsumoto et al ., 2020; Xu et al ., 2020b). Indeed, our study further shows that the mRNA levels of enzymes involved in the TCA cycle were increased in OT19 cells supplemented with sodium pyruvate and sodium propionate, suggesting that TCA cycle was enhanced (Fig.…”

Section: Discussionmentioning

confidence: 99%

Growth promotion of a deep‐sea bacterium by sensing infrared light through a bacteriophytochrome photoreceptor

Liu

Shan

Zheng

et al. 2021

Environmental Microbiology

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Photoreceptors are found in all kingdoms of life and bacteriophytochromes (Bphps) are the most abundant photo-sensing receptors in bacteria. Interestingly, BphPs have been linked to some bacterial physiological responses, yet most of the biological processes they regulate are still elusive, especially in non-photosynthetic bacteria. Here, we show that a bacteriophytochrome (CmoBphp) from a deep-sea bacterium Croceicoccus marinus OT19 perceives infrared light (wavelength at 940 nm) and transduces photo-sensing signals to a downstream intracellular transduction cascade for better growth. We discover that the infrared light-mediated growth promotion of C. marinus OT19 is attributed partly to the enhancement of pyruvate and propanoate metabolism. Further study suggests that CmoBphp plays a crucial role in integrating infrared light with intracellular signalling to control the bacterial growth and metabolism. This is the first report that deep-sea nonphotosynthetic bacteria can sense infrared light to control growth through a bacteriophytochrome photoreceptor, thus providing new understandings towards light energy utilization by microorganisms.

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Phytoremediation of cadmium-polluted soil assisted by D-gluconate-enhanced Enterobacter cloacae colonization in the Solanum nigrum L. rhizosphere

Cited by 65 publications

References 78 publications

Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton

Metabolome and transcriptome association analysis revealed key factors involved in melatonin mediated cadmium-stress tolerance in cotton

Effect of soil management systems on the rhizosphere bacterial community structure of tobacco: Continuous cropping vs. paddy-upland rotation

Growth promotion of a deep‐sea bacterium by sensing infrared light through a bacteriophytochrome photoreceptor

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