Phytoscreening of Vochysiaceae species: Molecular identification by HPLC-ESI-MS/MS and evaluating of their antioxidant activity and inhibitory potential against human α-amylase and protein glycation

Justino, Allisson Benatti; Martins, Mário Machado; Silva, C.G.; Campana, Priscilla Rodrigues Valadares; Lopes, Júlio César Dias; Almeida, Vera Lúcia de; Espindola, Foued Salmen

doi:10.1016/j.bioorg.2019.103122

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…These results are in agreement with those obtained by R.R Franco and coll. (2019) who showed strong antioxidant activities of several plants belonging to the Vochysiaceae family [ 5 ]. In fact, they are even more interesting because all of the activities observed by Franco and colleagues (2019) are lower than the antioxidant capacity of ascorbic acid regarding the FRAP test, while the extract and fractions obtained from Erismadelphus exsul are four times more antioxidant than ascorbic acid, suggesting a putative interest for industrial valorization such as nutraceutical potential.…”

Section: Resultsmentioning

confidence: 99%

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Combined LC-MS/MS and Molecular Networking Approach Reveals Antioxidant and Antimicrobial Compounds from Erismadelphus exsul Bark

Mintsa

N’Nang

Choque

et al. 2022

Plants

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Erismadelphus exsul Mildbr bark is widely used in Gabonese folk medicine. However, little is known about the active compounds associated with its biological activities. In the present study, phytochemical profiling of the ethanolic extract of Erismadelphus exsul was performed using a de-replication strategy by coupling HPLC-ESI-Q/TOF with a molecular network approach. Eight families of natural compounds were putatively identified, including cyclopeptide alkaloids, esterified amino acids, isoflavonoid- and flavonoid-type polyphenols, glycerophospholipids, steroids and their derivatives, and quinoline alkaloids. All these compounds were identified for the first time in this plant. The use of molecular networking obtained a detailed phytochemical overview of this species. Furthermore, antioxidant (2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH) and ferric reducing capacity (FRAP)) and in vitro antimicrobial activities were assessed. The crude extract, as well as fractions obtained from Erismadelphus exsul, showed a better reactivity to FRAP than DPPH. The fractions were two to four times more antioxidant than ascorbic acid while reacting to FRAP, and there was two to nine times less antioxidant than this reference while reacting to DPPH. In addition, several fractions and the crude extract exhibited a significant anti-oomycete activity towards the Solanaceae phytopathogen Phytophthora infestans in vitro, and, at a lower extent, the antifungal activity against the wheat pathogen Zymoseptoria tritici had growth inhibition rates ranging from 0 to 100%, depending on the tested concentration. This study provides new insights into the phytochemical characterization and the bioactivities of ethanolic extract from Erismadelphus exsul bark.

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Section: Resultsmentioning

confidence: 99%

“…The work of Franco and coll. also demonstrated the antioxidant and anti-glycation capacity of plant extracts from the Vochysiaceae family [ 5 ]. In addition, Dornelo and coll.…”

Section: Introductionmentioning

confidence: 99%

Combined LC-MS/MS and Molecular Networking Approach Reveals Antioxidant and Antimicrobial Compounds from Erismadelphus exsul Bark

Mintsa

N’Nang

Choque

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…The liver homogenates were incubated with 300 mM sodium acetate buffer (pH 3.6), 10 mM 2,4,6-tri (2pyridyl) -striazine (TPTZ) and 20 mM ferric chloride at 37 ºC for 6 min, at 593 nm. A trolox analytical curve determined the antioxidant capacity and sodium acetate buffer was used as a blank (22).…”

Section: Total Antioxidant Capacitymentioning

confidence: 99%

Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Sommerfeld

Mundim

Silva

et al. 2021

Preprint

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Background: Several works have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardise the evaluated parameters are needed to better understand and identify the viability of chicken embryos (CE) as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are peculiar to the model. To achieve this goal, in ovo assays were performed injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult chickens and other animals. Results: Embryo response to virus and drug challenges may not occur as expected for adult chickens and even different species. While macroscopic and microscopic damage was visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not result in the expected effects for CE. On the other hand, with dexamethasone (DX), changes in blood parameters and biomarkers were inherent to the model and depended on the stage of CE development.Conclusions: Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are recorded.

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Section: Characterisation Of Blood Cellsmentioning

confidence: 99%

Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Sommerfeld

Mundim

Silva

et al. 2021

Preprint

View full text Add to dashboard Cite

Several works have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardise the evaluated parameters are needed to better understand and identify the viability of chicken embryos (CE) as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are peculiar to the model. To achieve this goal, in ovo assays were performed injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult chickens and other animals. Embryo response to virus and drug challenges may not occur as expected for adult chickens and even different species. While macroscopic and microscopic damage was visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not result in the expected effects for CE. On the other hand, with dexamethasone (DX), changes in blood parameters and biomarkers were inherent to the model and depended on the stage of CE development. Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are recorded.

show abstract

Phytoscreening of Vochysiaceae species: Molecular identification by HPLC-ESI-MS/MS and evaluating of their antioxidant activity and inhibitory potential against human α-amylase and protein glycation

Cited by 13 publications

References 37 publications

Combined LC-MS/MS and Molecular Networking Approach Reveals Antioxidant and Antimicrobial Compounds from Erismadelphus exsul Bark

Combined LC-MS/MS and Molecular Networking Approach Reveals Antioxidant and Antimicrobial Compounds from Erismadelphus exsul Bark

Physiological Changes in Chicken Embryos Inoculated With Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardisation of This Animal Model

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