Phytosphingosine inhibits cell proliferation by damaging DNA in human cell lines

Sun, Chunxiao; Chang, Xuexiu; MacIsaac, Hugh J.; Wen, Jiayao; Zhao, Li‐Xing; Dai, Zhi; Li, Jiaojiao

doi:10.1016/j.ecoenv.2023.114840

Cited by 5 publications

(2 citation statements)

References 57 publications

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“…At present, more than 2000 chemicals other than phycotoxins were identified from the secondary metabolites of M. aeruginosa , including lipids, organic heterocyclic compounds, organic acids, benzene-like compounds, and organic oxygen compounds 8 , 9 , 11 . Although several chemicals have been screened as potential reason of neurotoxicity (such as phytosphingosine, egonol glucoside, and dihydrosuberenol) 75 , reproductive interference (such as 8-iso-15-keto-PGE2, ricinoleic acid, and oleic acid), immunotoxicity (such as shinflavanone, stearidonic acid, and linoleamide) 10 , and cytotoxicity (phytosphingosine) 76 , 77 caused by MaE, the toxic effect of MaE on aquatic organisms is the result of integrated toxicity of all the secondary metabolites. Therefore, it is still necessary to focus on the integrated ecotoxicological effects of extracellular secretions of cyanobacteria on organisms, especially which system and organ function of the organism is influenced the most 12 .…”

Section: Discussionmentioning

confidence: 99%

Adverse effects of Microcystis aeruginosa exudates on the filtration, digestion, and reproduction organs of benthic bivalve Corbicula fluminea

Hong,

Chen,

et al. 2024

Sci Rep

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Cyanobacteria bloom and the secondary metabolites released by the microorganism are extremely harmful to aquatic animals, yet study on their adverse effects in zoobenthos is rare. Corbicula fluminea widely distributed in freshwater environment with algal blooms. It is a typical filter feeding zoobenthos that may be affected by the secondary metabolites of cyanobacteria due to its high filtering rate. In this study, C. fluminea was exposed to Microcystis aeruginosa exudates (MaE) for 96 h, which was obtained from 5 × 105 cells/mL and 2.5 × 106 cells/mL exponential stage M. aeruginosa culture solution that represented cyanobacteria cell density needs environmental risk precaution control and emergent control, respectively. The responses of C. fluminea critical organs to MaE were analyzed and evaluated based on histopathological sections, antitoxicity biomarkers, and organ function biomarkers. The results showed that all the organs underwent structural disorders, cell vacuolization, apoptosis, and necrosis, and the damage levels increased as MaE concentration increased. The detoxification and antioxidant defense systems biomarkers in each organ response to MaE exposure differently and the level of reaction improved when MaE concentration increased. The siphon rate and acetylcholinesterase activity showed that the filtration function decreased significantly as the MaE concentration increased. Increased activity of glutathione S-transferase and amylase in the digestive gland indicate that it is the major detoxification organ of C. fluminea. Increased vitellogenin concentration and enlarged oocytes in the gonad indicate that MaE may have an estrogenic effect on C. fluminea. This study demonstrates that cyanobacteria threat benthic bivalves by inducing oxidative stress, inhibiting filtering feeding system, and disturbing digestion system and reproduction potential of C. fluminea.

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Section: Discussionmentioning

confidence: 99%

Adverse effects of Microcystis aeruginosa exudates on the filtration, digestion, and reproduction organs of benthic bivalve Corbicula fluminea

Hong,

Chen,

et al. 2024

Sci Rep

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“…They can also directly or indirectly interfere with mitochondria and induce apoptosis ( 57 ) and promote apoptosis by disrupting mitochondrial autophagy ( 54 , 58 ). Furthermore, PHS causes abnormal nuclear morphology, micronuclei and DNA damage, inhibits cell proliferation by damaging DNA, and activates the ATM/P53/p21 pathway, resulting in cell cycle arrest in S phase ( 59 ). In this experiment, we found that the metabolites of PHS and its analog (2-Amino-1,3-octadecanediol) in the mammary epithelial cells of dairy cows under high temperature conditions were significantly downregulated by 32 mmol/L taurine.…”

Section: Discussionmentioning

confidence: 99%

Effects of taurine on metabolomics of bovine mammary epithelial cells under high temperature conditions

Liu,

Liang,

Luo

et al. 2024

Front. Vet. Sci.

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High temperature induces heat stress, adversely affecting the growth and lactation performance of cows. Research has shown the protective effect of taurine against hepatotoxicity both in vivo and in vitro. This study aimed to investigate the effect of taurine on the metabolomics of mammary epithelial cells of dairy cows under high-temperature conditions. Mammary epithelial cells were exposed to 0 mmol/L (HS, control), 8 mmol/L (HT-8), and 32 mmol/L (HT-32) of taurine, then incubated at 42°C for 6 h. Metabolomics analysis was conducted using Liquid Chromatograph Mass Spectrometer (LC–MS). Compared with the HS group, 2,873 and 3,243 metabolites were detected in the HT-8 group in positive and negative ion modes. Among these, 108 and 97 metabolites were significantly upregulated in positive and negative ion modes, while 60 and 166 metabolites were downregulated. Notably, 15 different metabolites such as palmitic acid, adenine and hypoxanthine were screened out in the HT-8 group. Compared with the HS group, 2,873 and 3,243 metabolites were, respectively, detected in the HT-32 group in the positive and negative ion modes. Among those metabolites, 206 metabolites were significantly up-regulated, while 206 metabolites were significantly downregulated in the positive mode. On the other hand, 497 metabolites were significantly upregulated in the negative mode, while 517 metabolites were reported to be downregulated. Noteworthy, 30 distinct metabolites, such as palmitic acid, phytosphingosine, hypoxanthine, nonanoic acid, and octanoic acid, were screened out in the HT-32 group. KEGG enrichment analysis showed that these metabolites were mainly involved in lipid metabolism, purine metabolism and other biological processes. Overall, our study indicates that taurine supplementation alters the metabolites primarily associated with purine metabolism, lipid metabolism and other pathways to alleviate heat stress in bovine mammary epithelial cells.

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Metagenomic and transcriptome elucidating the sulfamethoxazole degradation and metabolism pathways in fermentative bacteria and microalgae coupling system for mariculture wastewater treatment

Fan,

Wang,

Sun

et al. 2023

Chemical Engineering Journal

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Phytosphingosine inhibits cell proliferation by damaging DNA in human cell lines

Cited by 5 publications

References 57 publications

Adverse effects of Microcystis aeruginosa exudates on the filtration, digestion, and reproduction organs of benthic bivalve Corbicula fluminea

Adverse effects of Microcystis aeruginosa exudates on the filtration, digestion, and reproduction organs of benthic bivalve Corbicula fluminea

Effects of taurine on metabolomics of bovine mammary epithelial cells under high temperature conditions

Metagenomic and transcriptome elucidating the sulfamethoxazole degradation and metabolism pathways in fermentative bacteria and microalgae coupling system for mariculture wastewater treatment

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