Phytosterols isolated from endophytic fungus Colletotrichum gloeosporioides(Melanconiaceae)

Carvalho, Josiwander Miranda; Dolabela, Maria Fâni; Marinho, Patrícia Santana Barbosa; Marinho, Andrey Moacir do Rosário

doi:10.1590/1809-4392201500072

Cited by 25 publications

(9 citation statements)

References 17 publications

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“…Colletotrichum spp. was found to be the source of "phytosterols" [26]. Thus, the analysis confirms marine fungi are potential due to their adaptation in salt-rich environment.…”

Section: Analysis Of Mycoflorasupporting

confidence: 55%

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

2018

J App Biol Biotech

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Keratin derived from poultry, and other keratinous waste remains a headache to the human society and also to the nature. Marine fungi -an untapped resource to be exploited specifically for the production of keratinase. In this connection, marine sediment samples were randomly collected from Kovalam located along the south west coast of Tamil Nadu. Two marine-derived fungi were isolated from the sediment samples. The internal transcribed spacer region of the isolates was sequenced, submitted to the GenBank and an individual accession number obtained for Colletotrichum capsici (KY817475) and Curvularia lunata (KY828216). Keratinase enzyme was produced with C. capsici and C. lunata at pH 8.5, and total protein was estimated. The amount of protein produced by C. capsici was found to be 0.91 mg/ml and by C. lunata was 1.06 mg/ml. The molecular weight of keratinase was determined as 35kDa. Four parameters through pH, concentration of feather meal, carbon source, and nitrogen source were optimized during the present study. Keratinase production media inoculated with C. capsici and C. lunata exhibit OD max with respect to feather meal concentration and corresponding OD was 1.264 mg/ml and 0.639 mg/ml. The OD max for C. capsici and C. lunata supplemented with maltose as carbon source was 1.281 mg/ml and 1.426 mg/ml. The OD max for C. capsici and C. lunata supplemented with yeast extract as nitrogen source and corresponding OD was 1.858 mg/ml and 1.613 mg/ml. Thus, we conclude that marine-derived fungi C. capsici and C. lunata are potential producers of ecovaluable keratinase which could be used up to treat the ecological burden created by keratinous waste. INTRODUCTIONSoil-borne microfungi are reported to synthesize a wide variety of hydrolytic enzymes. Still several species are exploited in the industries toward the production of industrially important enzymes such as cellulase, pectinase, carbohydrase, and lipase. A unique class of enzyme called keratinase produced by microfungi population has both biotechnological, biomedical potentials, and ecological benefits. Some clinically important pathogenic dermatophytes such as Trichophyton spp.[1] produce this enzyme to invade the skin tissues. On the other hand, the microbial keratinase has ecovaluable role since it is being applied in the bioconversion of poultry and other keratinous waste. For this purpose keratinases trapped from Streptomyces spp. [2,3] and Bacillus spp. [4,5] have been investigated. Around 24 billion chickens were killed annually consequently 8.5 billion tone of poultry feather *Corresponding Author: Samuel Ponpandian, Department of Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu, India. Email: drsamuelponpandian@rediffmail.com waste are generated. As per a recent citation from India's leading newspaper that our country alone contributes 350 million tone of poultry waste. Discarded feather causes several ailments to human beings such as chlorosis, mycoplasmosis, and fowl cholera [6]. Poultry waste drenched during the ...

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“…Colletotrichum spp. was found to be the source of "phytosterols" [26]. Thus, the analysis confirms marine fungi are potential due to their adaptation in salt-rich environment.…”

Section: Analysis Of Mycoflorasupporting

confidence: 55%

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

2018

J App Biol Biotech

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“…: fusarentine 6,7-dimethyl ether, monocerin, and colletotrialide (Tianpanich et al, 2011), stigmasterol, sitostenone, squalene, ergosterol and ergosterol peroxide (Carvalho et al, 2016) were identified. Colletotrichum species have been identified to produce a variety of secondary metabolites genes, including flavones, peptides and terpenes (Crouch et al, 2014) and many other metabolites as Jayawardena et al (2016) shows.…”

Section: Discussionmentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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Lippia sidoidesCham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC 50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

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“…16 The sterols ergosterol (5), ergosterol peroxide (6), cerivisterol (7), sitosterol (8), and stigmasterol (9) were isolated by chromatographic procedures and identified by NMR and with literature data. [17][18][19] Analysis of the control extracts by MS showed the absence of the isolated compounds in the culture medium, confirming that the isolated compounds are secondary metabolites produced by the fungus Aspergillus FRIZ12 and not from the culture medium.…”

Section: Isolated Compoundsmentioning

confidence: 76%

Chemical Constituents and Antimicrobial Activity of Soil Fungus Aspergillus sp. FRIZ12

et al. 2018

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Constituintes Químicos e Atividade Antimicrobiana do Fungo de Solo Aspergillus sp. FRIZ12 Resumo: O presente trabalho relata o isolamento de nove compostos da biomassa do fungo de solo Aspergillus sp. FRIZ12. Os compostos 2-benzil-4H-piran-4-ona (1), flavasperona (2), 5-(hidroximetil) furfural (3), uridina (4), ergosterol (5), peróxido de ergosterol (6), cerivisterol (7), estigmasterol (8) e sitosterol (9) foram isolados por métodos cromatográficos, e as estruturas foram identificadas por RMN, EM e comparação com dados da literatura. O composto 1 está sendo relatado pela primeira vez no gênero Aspergillus e pela segunda vez como um produto natural. Os compostos 1 e 2 mostraram moderada atividade antimicrobiana

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Phytosterols isolated from endophytic fungus Colletotrichum gloeosporioides(Melanconiaceae)

Cited by 25 publications

References 17 publications

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

Bio-prospecting of marine-derived fungi with special reference to production of keratinase enzyme - A need-based optimization study

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Chemical Constituents and Antimicrobial Activity of Soil Fungus Aspergillus sp. FRIZ12

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