Phytosynthesis of Ag nanoparticles from Mentha longifolia: their structural evaluation and therapeutic potential against HCT116 colon cancer, Leishmanial and bacterial cells

Javed, Bilal; Nadhman, Akhtar; Mashwani, Zia‐ur‐Rehman

doi:10.1007/s13204-020-01428-5

Cited by 40 publications

(24 citation statements)

References 45 publications

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“…The UV-Vis spectrophotometry (Systronics HALO DB-20, Dynamica Scientific Ltd.) was used to confirm the synthesis of SeNPs by measuring the wavelength of the reaction sample in the range of 200-800 nm of the light wavelength [22]. FTIR investigation was performed in the range of 400-4,000 cm −1 by employing an FTIR spectrometer with a resolution of 0.15 cm −1 to identify the functional groups that are responsible to stabilize SeNPs [23].…”

Section: Characterization Of Biosynthesized Senpsmentioning

confidence: 99%

Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

Ikram

Raja

Javed

et al. 2020

Green Processing and Synthesis

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The present study was aimed to biosynthesize selenium nanoparticles (SeNPs) and assess their foliar applications to improve the growth of wheat plants under controlled irrigation and drought stress. Bud aqueous extract of Allium sativum L. was used as a reducing and stabilizing agent of SeNPs followed by their optical and morphological characterization by using ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier-transform infrared, and energy dispersive X-ray analysis. Various concentrations of SeNPs (10, 20, 30, and 40 mg/L) were applied exogenously to drought-tolerant (V1) and drought-susceptible (V2) wheat varieties at the trifoliate stage. Under the positive control conditions, plants were irrigated with 450 mL of water/pot (100% field capacity); and under water-deficit environment, plants were irrigated with 160 mL of water/pot (35% field capacity). Remarkable increase in plant height, shoot length, shoot fresh weight, shoot dry weight, root length, root fresh weight, root dry weight, leaf area, leaf number, and leaf length has been observed when 30 mg/L concentration of SeNPs was used. However, the plant morphological parameters decreased gradually at higher concentrations (40 mg/L) in both selected wheat varieties. Therefore, 30 mg/L concentration of SeNPs was found most preferable to enhance the growth of selected wheat varieties under normal and water-deficient conditions.

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Section: Characterization Of Biosynthesized Senpsmentioning

confidence: 99%

Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

Ikram

Raja

Javed

et al. 2020

Green Processing and Synthesis

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“…Generally, nanotechnology is a base of novel applications such as nanomaterials, nanometrology, electronics, optoelectronics, nanobiotechnology and industrial applications 12 , 13 . In particularly, AgNPs have been used as delivery tool in gene and colon cancer therapies 14 , 15 as well as antibacterial activities 16 – 18 . Therefore, AgNPs could be a promising approach to treat MDR because it is rarely to find microbes that resist AgNPs since microbes need various mutation to develop such phenomenon 19 because of metals several targets in microbe 20 .…”

Section: Introductionmentioning

confidence: 99%

Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

Alqahtani

Othman

Mohammed

2020

Sci Rep

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Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat to human health. One of the useful materials for bacterial control is Silver nanoparticles (AgNPs). Researchers tend to use biogenic agents to synthesize stable and safe AgNPs. The principal aim of this study was to investigate the ability of lichen in AgNPs formation and to find out their suppression ability to MDR bacteria as well as their cytotoxic activity. In the current study, lichens (Xanthoria parietina, Flavopunctelia flaventior) were collected from the south of the Kingdom of Saudi Arabia. Lichens methanolic extracts were used for conversion of Ag ions to AgNPs. Prepared biogenic AgNPs were characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential and Energy-Dispersive X-ray Spectroscopy (EDS). Lichens Secondary metabolites were determined by Fourier-Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS). The antibacterial activity and synergistic effect of AgNPs were evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), and gram-negative; (Pseudomonas aeruginosa, Escherichia coli) as well as the reference strains (ATCC) using the agar disk diffusion method. Cytotoxic effect of biogenic AgNPs was tested against HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer cell), and FaDu (Pharynx cancer cell) by MTT test. TEM imaging showed well-dispersed spherical particles of 1–40 nm size as well as zeta size showed 69–145 nm. Furthermore, FTIR and GC–MS identified various lichen chemical molecules. On the other hand, the highest antibacterial activity of AgNPs was noticed against P. aeruginosa, followed by MRSA, VRE, and E. coli. AgNPs influence on gram-negative bacteria was greater than that on gram-positive bacteria and their synergistic effect with some antibiotics was noted against examined microbes. Moreover, higher cytotoxicity for biogenic AgNPs against FaDu and HCT 116 cell line in relation to MDA-MB-231 was noted. Given the current findings, the biogenic AgNPs mediated by lichens had positive antibacterial, synergistic and cytotoxic powers. Therefore, they might be considered as a promising candidate to combat the multi-drug resistance organisms and some cancer cells.

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“…The biogenesis of nanoparticles can be steered by using different physicochemical reaction conditions. The pH along with the temperature and the molar concentration of silver salt plays a vital role to affect the morphology and the biological covering of the metallic nanoparticles [16]. The pH scale is divided into three basic parts acidic, neutral and basic so the synthesis of nanoparticles was tested against all three types of pH conditions.…”

Section: Role Of Different Ph Ranges and Concentrations Of Agnomentioning

confidence: 99%

“…Almost 50% of the Leishmanial population was found dead by using 10 mg/ml of SNPs which is showing that these nanoparticles are highly biocompatible as a very small dosage is required to affect the growth of pathogen and results are statistically significant (p < .05). The cytotoxicity of SNPs is usually because of the presence of a positive charge on silver ion that interacts with the negatively charged plasma cell membrane and disrupts the ionic balance and finally the membrane structure [16,17]. Another reason is the small size of nanoparticles that have the advantage to cross the plasma cell membrane barrier of the pathogenic organisms and enter the cytoplasm where it disrupts the normal cells' biochemical processes.…”

Section: Dose-responsive Potential Of Snps Against Leishmania Tropicamentioning

confidence: 99%

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Synergistic response of physicochemical reaction parameters on biogenesis of silver nanoparticles and their action against colon cancer and leishmanial cells

Javed

Mashwani

Sarwer

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Physicochemical parameters include pH, temperature, the concentration of the AgNO 3 , ratio of reactants, agitation and incubation period that act synergistically and provide a steering force to modulate the biogenesis of nanoparticles by influencing the molecular dynamics, reaction kinetics, protein conformations, and catalysis. The current study involved the bio-fabrication of silver nanoparticles (SNPs) by using the reducing abilities of Mentha longifolia (L.) L. leaves aqueous extract. Spectrophotometric analysis of various biochemical reactions showed that 3 mM of AgNO 3 at 120 C in an acidic pH when mixed in 1-9 ratio of plant extract and AgNO 3 respectively, are the optimised conditions for SNPs synthesis. Different analytical techniques confirmed that the nanoparticles are anisotropic and nearly spherical and have a size range of 10-100 nm. The $10 mg/ml of SNPs killed $66% of Leishmania population and IC 50 was measured at 8.73 mg/ml. SRB assay and Annexin V apoptosis assay results showed that the plant aqueous extract and SNPs are not active against HCT116 colon cancer cells and no IC 50 (80% survival) was reported. ROS generation was quantified at 0.08 U, revealed that the SNPs from M. longifolia can generate free radicals and no photothermal activity was recorded which makes them non-photodynamic.

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Phytosynthesis of Ag nanoparticles from Mentha longifolia: their structural evaluation and therapeutic potential against HCT116 colon cancer, Leishmanial and bacterial cells

Cited by 40 publications

References 45 publications

Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

Foliar applications of bio-fabricated selenium nanoparticles to improve the growth of wheat plants under drought stress

Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

Synergistic response of physicochemical reaction parameters on biogenesis of silver nanoparticles and their action against colon cancer and leishmanial cells

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