Phytosynthesis of eco-friendly silver nanoparticles and biological applications A novel concept in nanobiotechnology

Reddy, M Sunitha; Murthy, K. Sri Rama; Srilakshmi, A; Rao, Krs Sambasiva; Pullaiah, T

doi:10.5897/ajb2013.13299

Cited by 22 publications

(2 citation statements)

References 78 publications

(95 reference statements)

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“…Generally, it is common that due to SPR, silver nano-crystals showed a typical absorption peak at 3 keV in EDX (Magudapatty et al, 2001). Some other minor peaks in graph may be possible due to the presence of biomolecules in plant material bound to AgNPs (Reddy et al, 2015). The SEM results of the current study are in accordance with the work of Mehmood et al (2017), who synthesized predominantly spherical shaped AgNPs from M. azedarach extract.…”

Section: Discussionsupporting

confidence: 88%

Microwave-Assisted Green Synthesis and Characterization of Silver Nanoparticles Using Melia azedarach for the Management of Fusarium Wilt in Tomato

et al. 2020

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Section: Discussionsupporting

confidence: 88%

Microwave-Assisted Green Synthesis and Characterization of Silver Nanoparticles Using Melia azedarach for the Management of Fusarium Wilt in Tomato

et al. 2020

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“…18 Because of the binding of biomolecules to the surface of Ag/AgCl-NPs, weak signals were formed. 19 The first weight loss (3%) that appeared within temperature 100 °C, indicated the removal of water, and the second and third weight loss (40.28%) suggested the elimination or decomposition of the capping biomolecules as observed by TGA. 20 This result indicated that the synthesized nanoparticles possessed high thermal stability.…”

Section: ■ Discussionmentioning

confidence: 93%

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

et al. 2020

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Recently, green synthesis of silver/silver chloride nanoparticles (Ag/AgCl-NPs) has gained a lot of interest because of the usage of natural resources, rapidness, eco-friendliness, and benignancy. Several researchers reported that silver-based biogenic NPs have both antimicrobial and anticancer properties. In the present study, Ag/AgCl-NPs were synthesized from Zizyphus mauritiana fruit extract, and their antibacterial, antifungal, and antiproliferative mechanisms against human MCF-7 cell lines were evaluated. Synthesis of Ag/AgCl-NPs from the Z. mauritiana fruit extract was confirmed by the changes of color and a peak of the UV–visible spectrum at 428 nm. The nanoparticles were characterized by transmission electron microscopy, energy dispersive X-ray, X-ray powder diffraction, thermal gravimetric analysis, atomic force microscope, and Fourier transform infrared. Antibacterial activity was checked against four pathogenic bacteria and two fungi. Cytotoxicity was checked against human breast cancer cell line (MCF-7) and mice Ehrlich ascites carcinoma (EAC) cells by MTS assay and clonogenicity assay. Cell morphology of the control and nanoparticle-treated MCF-7 cells were checked by Hoechst 33342, YF488-Annexin V, and caspase-3 substrates. The level of reactive oxygen species (ROS) was studied by using 2′,7′-dichlorofluorescein-diacetate staining. Real-time polymerase chain reaction was used for gene expression. Synthesized nanoparticles were heat stable cubic crystals with an average size of 16 nm that contain silver and chlorine with various functional groups. The synthesized Ag/AgCl-NPs inhibited the growth of three pathogenic bacteria ( Bacillus subtilis , Shigella boydii, and Escherichia coli ) and two fungi ( Aspergillus niger and Trichoderma spp.). Ag/AgCl-NPs inhibited the growth of MCF-7 and EAC cells with the IC 50 values of 28 and 84 μg/mL, respectively. No colony was formed in MCF-7 cells in the presence of these nanoparticles as compared with control. Ag/AgCl-NPs induced apoptosis and generated ROS in MCF-7 cells. The expression level of FAS, FADD, and caspase-8 genes increased several folds with the decrease of PARP gene expression. These results demonstrated that the anti-proliferation activity of Ag/AgCl-NPs against MCF-7 cells resulted through ROS generation and induction of apoptosis through the Fas-mediated pathway.

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Development of antibacterial and UV protective cotton fabrics using plant food waste and alien invasive plant extracts as reducing agents for the in-situ synthesis of silver nanoparticles

2021

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The development of cellulose-based textiles that are functionalised with silver nanoparticles (AgNP), synthesised according to a green approach, and offer protection against ultraviolet (UV) radiation and pathogenic bacteria is very important today. In the present work we demonstrate the environmentally friendly approach to obtain such textile material by AgNP synthesis directly (in-situ) on cotton fabrics, using water extracts of plant food waste (green tea leaves, avocado seed and pomegranate peel) and alien invasive plants (Japanese knotweed rhizome, goldenrod flowers and staghorn sumac fruit) as reducing agents. The extracts were analysed for their total content of phenols and flavonoids and their antioxidant activity. The synthesised AgNP on cotton were round, of different size and amount depending on the reducing agent used. The highest amount of AgNP was found for samples where Japanese knotweed rhizome extract was used as reducing agent and the lowest where extracts of goldenrod flowers and green tea leaves were used. Regardless of the reducing agent used to form AgNP, all cotton samples showed excellent protection against E. coli and S. aureus bacteria and against UV radiation with UV protection factor values above 50. The best results for UV protection even after the twelve repetitive washing cycles were found for the sample functionalized with AgNP synthesised with an extract of the Japanese knotweed rhizome. Due to the presence of AgNP on cotton, the air permeability and thermal conductivity decreased. AgNP had no effect on the change in breaking strength or elongation of fabrics. Graphic abstract

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Phytosynthesis of eco-friendly silver nanoparticles and biological applications A novel concept in nanobiotechnology

Cited by 22 publications

References 78 publications

Microwave-Assisted Green Synthesis and Characterization of Silver Nanoparticles Using Melia azedarach for the Management of Fusarium Wilt in Tomato

Microwave-Assisted Green Synthesis and Characterization of Silver Nanoparticles Using Melia azedarach for the Management of Fusarium Wilt in Tomato

Zizyphus mauritiana Fruit Extract-Mediated Synthesized Silver/Silver Chloride Nanoparticles Retain Antimicrobial Activity and Induce Apoptosis in MCF-7 Cells through the Fas Pathway

Development of antibacterial and UV protective cotton fabrics using plant food waste and alien invasive plant extracts as reducing agents for the in-situ synthesis of silver nanoparticles

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