Phytosynthesis of Silver Nanoparticles Using Myrtus communis L. Leaf Extract and Investigation of Bactericidal Activity

Abstract

Silver nanoparticles have been synthesized using only Myrtus communis L. leaf extract by a facile procedure without other reagents. The extract played the roles of both reducing and capping agent. The nanoparticles were characterized using field-emission scanning microscopy, and remained stable for at least 3 weeks. Antibacterial activity of the nanoparticles was evaluated toward Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, and Enterococcus faecalis based on inhibition zone disk diffusion assays. The minimum inhibitory and bactericidal concentrations of the nanoparticles were obtained. Mechanisms for the antibacterial activity were proposed.

Biogenic silver nanoparticles by Myrtus communis plant extract: biosynthesis, characterization and antibacterial activity

“…MIC value of synthesized aqueous silver nanoparticles against MRSA was 12.5 mg/mL, for E. coli -25 mg/mL in comparison to vancomycin -3 mg/ mL and gentamicin -6 mg/mL (Table 2). Ajdari et al [24] reported MIC value of E. coli 24.5 mg/mL and of MRSA -16.3 mg/mL against silver nanoparticles from M. communis leaf extract which coincided with our results. MBC was observed to be 25 mg/mL and 50 mg/ mL respectively for MRSA and E. coli in reference to 6 mg/mL for vancomycin and 12 mg/mL for gentamicin which were lower values obtained for our study as compared to Ajdari et al [24].…”

“…(A)) and E. coli (Figure5(B)) with respect to plant extracts. Recent study on Buffer extraction of M. communis showed strong sensitivity against Gram-positive bacteria[24].…”

Nickel-Doped Cerium Oxide Nanoparticles: Green Synthesis Using Stevia and Protective Effect against Harmful Ultraviolet Rays

“…Its sweet components are stevioside, steviol bioside, ribodiazide and dalcoside [27]. Recent investigations have shown that this plant has the ability to prepare in a fast and non-toxic way chemically stable iron oxide, zinc oxide and zinc sulfide nanoparticles [28][29][30][31][32][33].…”

Antimicrobial Photothermal Treatment of Pseudomonas Aeruginosa by a Carbon Nanoparticles-Polypyrrole Nanocomposite

“…Nanomaterials are developed due to exhibition of novel characteristics arising from their size-dependent features, and widely used in medical diagnoses and therapies [8][9][10]. These materials have a usage in biological and medical applications such as labeling of biomolecules [11], drug and gene delivery [12][13][14], rapid and sensitive microbial detections [15,16], antimicrobial materials [10,[17][18][19], detection and recognition of DNA and proteins [20][21][22], probing of intrinsic DNA structure [23], biologic substitutes and regenerative medicine [24], hyperthermia [25], separation of specific cell types [26] and MRI contrast agents [27]. Photothermal agents employed in PTT should absorb NIR light, while they possess photostability with no cytotoxicity.…”