Phytotoxicity and genotoxicity as a new approach to assess heavy metals effect on Medicago sativa L.: Role of metallo-resistant rhizobacteria

Raklami, Anas; Oubane, Mohamed; Meddich, Abdelilah; Hafidi, Mohamed; Marschner, Bernd; Heinze, Stefanie; Oufdou, Khalid

doi:10.1016/j.eti.2021.101833

Cited by 21 publications

(5 citation statements)

References 31 publications

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“…Previously, reduction in positive values of PI for the barley plant has been attributed to diluted concentrations of treated wastewater, which indicated removal of phenol and heavy metals [52]. In our work, crop irrigation with treated hospital wastewater leads to the reduction in PI value, which may also be due to various reasons: heavy metal reduction [63,64], reduction in Previously, the phytotoxicity assessment in plants has been carried out using one growth predicting factor, i.e., GI [62]. The present study is significantly different as it chose two growth predicting factors, GI and PI.…”

Section: Radish Cropsupporting

confidence: 54%

“…Previously, reduction in positive values of PI for the barley plant has been attributed to diluted concentrations of treated wastewater, which indicated removal of phenol and heavy metals [52]. In our work, crop irrigation with treated hospital wastewater leads to the reduction in PI value, which may also be due to various reasons: heavy metal reduction [63,64], reduction in phenols and supplementary organic complexes [65] and stress tolerances [66]. Radish roots are more sensitive towards the uptake of heavy metals and contaminants from the soil [67]; hence, the crop plant has more proximity towards inducing phytotoxicity.…”

Section: Radish Cropsupporting

confidence: 54%

“…Another two treated hospital wastewater concentration (50 and 75%) showed positive values of PI (0.21 and 0.39) (Figure 7b). In light of the available literature, heavy metal reduction [63,64], reduction of phenols and supplementary organic compounds [65] may be the possible reasons that contributed to the positivity in phytotoxicity values.…”

Section: Hot Pepper Cropmentioning

confidence: 99%

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Machine Learning Approach to Predict Quality Parameters for Bacterial Consortium-Treated Hospital Wastewater and Phytotoxicity Assessment on Radish, Cauliflower, Hot Pepper, Rice and Wheat Crops

Rashid

Mirza

Keating

et al. 2022

Water

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Raw hospital wastewater is a source of excessive heavy metals and pharmaceutical pollutants. In water-stressed countries such as Pakistan, the practice of unsafe reuse by local farmers for crop irrigation is of major concern. In our previous work, we developed a low-cost bacterial consortium wastewater treatment method. Here, in a two-part study, we first aimed to find what physico-chemical parameters were the most important for differentiating consortium-treated and untreated wastewater for its safe reuse. This was achieved using a Kruskal–Wallis test on a suite of physico-chemical measurements to find those parameters which were differentially abundant between consortium-treated and untreated wastewater. The differentially abundant parameters were then input to a Random Forest classifier. The classifier showed that ‘turbidity’ was the most influential parameter for predicting biotreatment. In the second part of our study, we wanted to know if the consortium-treated wastewater was safe for crop irrigation. We therefore carried out a plant growth experiment using a range of popular crop plants in Pakistan (Radish, Cauliflower, Hot pepper, Rice and Wheat), which were grown using irrigation from consortium-treated and untreated hospital wastewater at a range of dilutions (turbidity levels) and performed a phytotoxicity assessment. Our results showed an increasing trend in germination indices and a decreasing one in phytotoxicity indices in plants after irrigation with consortium-treated hospital wastewater (at each dilution/turbidity measure). The comparative study of growth between plants showed the following trend: Cauliflower > Radish > Wheat > Rice > Hot pepper. Cauliflower was the most adaptive plant (PI: −0.28, −0.13, −0.16, −0.06) for the treated hospital wastewater, while hot pepper was susceptible for reuse; hence, we conclude that bacterial consortium-treated hospital wastewater is safe for reuse for the irrigation of cauliflower, radish, wheat and rice. We further conclude that turbidity is the most influential parameter for predicting bio-treatment efficiency prior to water reuse. This method, therefore, could represent a low-cost, low-tech and safe means for farmers to grow crops in water stressed areas.

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Section: Radish Cropsupporting

confidence: 54%

“…Previously, reduction in positive values of PI for the barley plant has been attributed to diluted concentrations of treated wastewater, which indicated removal of phenol and heavy metals [52]. In our work, crop irrigation with treated hospital wastewater leads to the reduction in PI value, which may also be due to various reasons: heavy metal reduction [63,64], reduction in phenols and supplementary organic complexes [65] and stress tolerances [66]. Radish roots are more sensitive towards the uptake of heavy metals and contaminants from the soil [67]; hence, the crop plant has more proximity towards inducing phytotoxicity.…”

Section: Radish Cropsupporting

confidence: 54%

Section: Hot Pepper Cropmentioning

confidence: 99%

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Machine Learning Approach to Predict Quality Parameters for Bacterial Consortium-Treated Hospital Wastewater and Phytotoxicity Assessment on Radish, Cauliflower, Hot Pepper, Rice and Wheat Crops

Rashid

Mirza

Keating

et al. 2022

Water

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“…The use of PGPB may alter the metal accumulation capacity and restrict its translocation to a different part of the plant through the growth-promoting traits, such as metal resistance, translocation, accumulation, transformation and sequestration, thus reducing the availability of heavy metals in the contaminated soil [ 140 ]. Inoculation of Medicago sativa with bacterial strains plays an important role in improving seed germination, growth and reduced heavy metal stress by decreasing the antioxidant enzymes and PTEs’ accumulation content, finally improving the phytostabilization process efficiency [ 136 , 141 ]. Mycorrhizae also display their role in phytoremediation by restricting heavy metals on fungal mycelium through a physical barrier, thus reducing their bioavailability, translocation, and bioaccumulation in the plants [ 136 ].…”

Section: Recent Advances In Phytoremediationmentioning

confidence: 99%

Phytoremediation of Potentially Toxic Elements: Role, Status and Concerns

Wani

Ahmad

Asgher

et al. 2023

Plants

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Environmental contamination with a myriad of potentially toxic elements (PTEs) is triggered by various natural and anthropogenic activities. However, the industrial revolution has increased the intensity of these hazardous elements and their concentration in the environment, which, in turn, could provoke potential ecological risks. Additionally, most PTEs pose a considerable nuisance to human beings and affect soil, aquatic organisms, and even nematodes and microbes. This comprehensive review aims to: (i) introduce potentially toxic elements; (ii) overview the major sources of PTEs in the major environmental compartments; (iii) briefly highlight the major impacts of PTEs on humans, plants, aquatic life, and the health of soil; (iv) appraise the major methods for tackling PTE-caused pollution; (v) discuss the concept and applications of the major eco-technological/green approaches (comprising phytoextraction, rhizofiltration, phytostabilization, phytovolatilization, and phytorestoration); (vi) highlight the role of microbes in phytoremediation under PTE stress; and (vii) enlighten the major role of genetic engineering in advancing the phytoremediation of varied PTEs. Overall, appropriate strategies must be developed in order to stop gene flow into wild species, and biosafety issues must be properly addressed. Additionally, consistent efforts should be undertaken to tackle the major issues (e.g., risk estimation, understanding, acceptance and feasibility) in order to guarantee the successful implementation of phytoremediation programs, raise awareness of this green technology among laymen, and to strengthen networking among scientists, stakeholders, industrialists, governments and non-government organizations.

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“… Osuna-Vallejo et al (2019) , evaluated the capability of the conifers Juniperus deppeana and Pinus pseudostrobus for extracting mercury from soils contaminated by mining deposits, and found that both species accumulated the metal in their wood, representing excellent candidates for phytoremediation by bioacumulation of mercury. PGPR can improve this type of phytoremediation processes ( Raklami et al, 2019 , 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Arsenic and mercury tolerant rhizobacteria that can improve phytoremediation of heavy metal contaminated soils

Rojas-Solís¹,

Larsen²,

Lindig‐Cisneros³

2023

PeerJ

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Background Mining deposits often contain high levels of toxic elements such as mercury (Hg) and arsenic (As) representing strong environmental hazards. The purpose of this study was the isolation for plant growth promoting bacteria (PGPBs) that can improve phytoremediation of such mine waste deposits. Methods We isolated native soil bacteria from the rhizosphere of plants of mine waste deposits and agricultural land that was previously mine tailings from Tlalpujahua Michoacán, Mexico, and were identified by their fatty acid profile according to the MIDI Sherlock system. Plant growth promoting traits of all bacterial isolates were examined including production of 3-indoleacetic acid (IAA), siderophores, biofilm formation, and phosphate solubilization. Finally, the response of selected bacteria to mercury and arsenic was examined an in-vitro assay. Results A total 99 bacterial strains were isolated and 48 identified, representing 34 species belonging to 23 genera. Sixty six percent of the isolates produced IAA of which Pseudomonas fluorescens TL97 produced the most. Herbaspirillum huttiense TL36 performed best in terms of phosphate solubilization and production of siderophores. In terms of biofilm formation, Bacillus atrophaeus TL76 was the best. Discussion Most of the bacteria isolates showed high level of tolerance to the arsenic (as HAsNa2O4 and AsNaO2), whereas most isolates were susceptible to HgCl2. Three of the selected bacteria with PGP traits Herbispirillum huttiense TL36, Klebsiella oxytoca TL49 and Rhizobium radiobacter TL52 were also tolerant to high concentrations of mercury chloride, this might could be used for restoring or phytoremediating the adverse environmental conditions present in mine waste deposits.

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Phytotoxicity and genotoxicity as a new approach to assess heavy metals effect on Medicago sativa L.: Role of metallo-resistant rhizobacteria

Cited by 21 publications

References 31 publications

Machine Learning Approach to Predict Quality Parameters for Bacterial Consortium-Treated Hospital Wastewater and Phytotoxicity Assessment on Radish, Cauliflower, Hot Pepper, Rice and Wheat Crops

Machine Learning Approach to Predict Quality Parameters for Bacterial Consortium-Treated Hospital Wastewater and Phytotoxicity Assessment on Radish, Cauliflower, Hot Pepper, Rice and Wheat Crops

Phytoremediation of Potentially Toxic Elements: Role, Status and Concerns

Arsenic and mercury tolerant rhizobacteria that can improve phytoremediation of heavy metal contaminated soils

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