This research work explores the green synthesis of silver nanoparticles using Quassia indica(QI-Ag NPs), a natural plant extract, as a stabilizing and reducing agent. The synthesized QI-Ag NPs were characterized using various analytical techniques, including UV-Visible spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Transmission Electron Microscopy (HR-TEM) and Selected Area Electron Diffraction (SAED). The UV-Visible analysis revealed a characteristic peak at 430 nm, indicating the successful formation of AgNPs. XRD analysis unveiled the crystalline nature of the nanoparticles, with four distinctive peaks corresponding to the silver crystallographic planes. SEM and EDX provided insights into the morphology and chemical composition of the QI-AgNPs. Moreover, TEM and SAED elucidated the structural attributes and crystallinity of the nanoparticles. The Ag NPs exhibited a spherical structure and crystalline nature, as supported by both SAED and XRD findings. The zeta potential of QI-Ag NPs exhibited a value of −24.2 mV. The synthesized QI-Ag NPs were evaluated for their photocatalytic potential, demonstrating a remarkable 97% degradation of Crystal Violet dye. Furthermore, comprehensive studies encompassing antioxidant, antimicrobial and cytotoxicity assessments were conducted, showcasing the multifaceted applications of these nanoparticles. This research underscores the promising potential of Q. indica-mediated silver nanoparticles as environmentally benign and versatile nanomaterials.