Phytotoxicity Assay to Assess Plant Species for Phytoremediation of Petroleum-Contaminated Soil

Kirk, Jennifer L.; Klirnomos, John N.; Lee, Hung; Trevors, J. T.

doi:10.1080/10889860290777477

Cited by 52 publications

(30 citation statements)

References 18 publications

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“…presented by Kirk et al (2002) and Merkl et al (2004b), is limited. Results are furthermore influenced by variables like container size, water availability and fertilisation.…”

Section: Resultsmentioning

confidence: 94%

Assessment Of Tropical Grasses And Legumes For Phytoremediation Of Petroleum-Contaminated Soils

Merkl

Schultze-Kraft

Infante

2005

Water Air Soil Pollut

220

100

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Phytoremediation is a promising technology for the clean-up of petroleum-contaminated soils, especially in the tropics where climatic conditions favour plant growth and microbial activity and where financial resources can be limited. The objective of this work was to identify tropical plant species from the eastern savannahs of Venezuela suitable for this technology. Three legumes (Calopogonium mucunoides, Centrosema brasilianum, Stylosanthes capitata) and three grasses (Brachiaria brizantha, Cyperus aggregatus, Eleusine indica) were tested for their ability to stimulate microbial degradation in soil contaminated with 5% (w/w) of a heavy crude oil. In greenhouse experiments, plant biomass production and oil dissipation (total oil and grease, and fraction composition) were analysed after 90 and 180 days incubation. Although previously tested on their tolerance to oil contamination, the legumes died within six to eight weeks. The grasses showed reduced biomass production under the influence of the contaminant. Relative growth rates were higher in contaminated soil indicating a delay in plant growth patterns and development. Soil planted with B. brizantha and C. aggregatus showed a significantly lower oil concentration than non-vegetated soil. Furthermore, a positive correlation between root biomass production and oil degradation was found. Concentration of saturated hydrocarbons was always lower in planted than in unplanted soil. B. brizantha also caused a considerable reduction of aromatics. Based on these results, B. brizantha is recommended for follow-up investigations which could further develop the application of phytoremediation of petroleum-contaminated soils in the tropics.

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“…presented by Kirk et al (2002) and Merkl et al (2004b), is limited. Results are furthermore influenced by variables like container size, water availability and fertilisation.…”

Section: Resultsmentioning

confidence: 94%

Assessment Of Tropical Grasses And Legumes For Phytoremediation Of Petroleum-Contaminated Soils

Merkl

Schultze-Kraft

Infante

2005

Water Air Soil Pollut

220

100

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“…Using plants to control pollutants (phytoremediation) in the soil has become a widely accepted approach and consequently, plants viability in the zone of contamination is a critical issue in the successful application of phytoremediation. Selection of plants for remediation of soils with organic pollutants have been based on their resistance to pollutant phytotoxicity (Kirk et al, 2002), which manifests in their ability to germinate, grow and survive in the polluted medium. It is also based on the presence of enzymes and phenolic compounds in root exudate and plant tissues (Liste and Alexander, 1999) or their capability to reduce pollutant concentration in the soil.…”

mentioning

confidence: 99%

Evaluation on the growth response of Peanut (<i>Arachis hypogaea</i>) and Sorghum (<i>Sorghum bicolor</i>) to crude oil contaminated soil

Iheme¹,

Akinola²,

Njoku³

2017

Journal of Applied Sciences and Environmental Management

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This study examined the cytotoxic response, germination, survival, morphological deviations as well as enzyme activities of Arachis hypogaea and Sorghum bicolor in crude oil contaminated soil. Crude oil spillage was simulated to achieve 1%, 2%, 3% (w/w) contamination levels in pot experiments. Treatments without crude oil were used as control. Cytotoxicity, germination and survival were determined by using percentages while enzyme activity was measured by using spectrophotometric methods and standard curves. S. bicolor had lower mitotic index (3.7) with higher percentage aberrations (65.56%) compared to A. hypogaea. However, difference in mitotic index and percentage aberration between A. hypogaea and S. bicolor was not significant at P≥0.05. Percentage germination and survival of both plants were not different. Enzyme study showed that enzyme activity in A. hypogaea and S bicolor were the same in control but increased with crude oil contamination. Laccase activity was significantly higher (P≤0.05) in A. hypogaea than in S. bicolor in 1% and 2% crude oil concentrations. The same applies to soluble methane monoxygenase activity in all crude oil concentrations. Tyrosinase activity was not significantly different in both plants in all concentrations. The study shows A. hypogaea to have better tolerance in crude oil contaminated soils than S. bicolor. © JASEM https://dx

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“…Toxicity will vary with hydrocarbon types and levels, soil type and properties, and microbial population composition (Kirk et al 2002).…”

Section: Toxicity Testingmentioning

confidence: 99%

The Use of Goosegrass (Eleusine indica) to Remediate Soil Contaminated with Petroleum

Zhang

Sun

et al. 2009

Water Air Soil Pollut

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A greenhouse experiment was performed to evaluate effectiveness of goosegrass (Eleusine indica) in phytoremediation of soil contaminated with 8,247 mg kg −1 of total petroleum hydrocarbons (TPH). We determined seed germination toxicity, soil microbial viable counts, catalase activity, dehydrogenase activity (DHA), and the concentrations of TPH and 14 polycyclic aromatic hydrocarbons (PAHs) in soil and plant tissue. After 5 months, the initial level of contamination was reduced by 47% in planted soil, whereas it was only reduced by 11% in nonplanted soil. Bacterial numbers were 72 times greater in the rhizosphere treated soil than in the unvegetated treatment at the end of the study. There was no correlation between microbial counts or DHA and catalase activity, and the correlation between microbial counts and DHA was weak. Significant chemical reduction of H 2 O 2 , caused by the soil fabric, was observed in the determination of catalase activity. In case of vegetated treatment, 32% of PAHs was removed, but only 5% of PAHs was dissipated in the unvegetated pots. Gas chromatography/mass spectrometry analysis of plant tissue indicated that a low amount of PAHs (25.50 mg kg −1 dry biomass) was detected in goosegrass roots growing in the contaminated soil, and no uptake into the shoots was occurring.

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Phytotoxicity Assay to Assess Plant Species for Phytoremediation of Petroleum-Contaminated Soil

Cited by 52 publications

References 18 publications

Assessment Of Tropical Grasses And Legumes For Phytoremediation Of Petroleum-Contaminated Soils

Assessment Of Tropical Grasses And Legumes For Phytoremediation Of Petroleum-Contaminated Soils

Evaluation on the growth response of Peanut (<i>Arachis hypogaea</i>) and Sorghum (<i>Sorghum bicolor</i>) to crude oil contaminated soil

The Use of Goosegrass (Eleusine indica) to Remediate Soil Contaminated with Petroleum

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