Phytotoxicity of extracts and fractions of Ouratea spectabilis (Mart. ex Engl.) Engl. (Ochnaceae)

Abstract

Edited by L VerschaeveKeywords: Phytotoxic Allelopathy Allelochemicals HPLC -PAD Allium cepa DPPH Among the numerous plant species occurring in the Cerrado, Ouratea spectabilis stands out because of the lack of species that grow beneath its canopy. Therefore, this study aimed to evaluate the phytotoxic potential of different extracts and fractions of the hydroethanolic extract from leaves of O. spectabilis through laboratory bioassays of the pre-and post-emergence of seeds of Lactuca sativa L., determination of the mitotic index in root cells of Allium cepa L., antioxidant activity and phytochemical screening of different classes present in extracts and ethyl acetate fractions. It was possible to verify that different extracts and ethyl acetate fractions of O. spectabilis interfered with germination rates, as reduced germination was observed when compared with the control. Similarly, growth and development was affected in lettuce seedlings, as shown by the reduced length of primary roots and hypocotyls compared with the control. In addition, the mitotic index was reduced in treated groups compared with the negative control. HPLC-PAD analysis for both the hydroethanolic extract and its ethyl acetate fraction, showed a predominance of flavonoid compounds belonging to the groups of isoflavones and catechins in ethyl acetate fractions of hydroethanolic extracts. Thus, it was concluded that this species synthesizes phytotoxic compounds capable of interfering in the stabilization and development of other species.

(3,3″)-Linked Biflavanones from Ouratea spectabilis and Their Effects on the Release of Proinflammatory Cytokines in THP-1 Cells

“…Engl. (Ochnaceae) is an arborous species popularly known as folha de serra , occurring mainly in the Cerrado , a Brazilian savanna . The species is traditionally used as an anti-inflammatory agent to treat gastric ulcers and rheumatism .…”

Phytochemical prospection of the floral extract of Ouratea spectabilis (Mart.) Engl. (Ochnaceae)

“…Especially O. spectabilis presents other important biological activities from leaf extracts and their fractions, with an allelopathic effect on lettuce seeds and cytotoxic effect on the mitotic index in the study by Mecina et al (2014). Molecules isolated from the leaf extract of O. spectabilis showed an inhibitory effect on aldose reductase (AR) from two phytocompounds.…”

“…Engl. (Figure 1) is described in areas of Cerrado sensu stricto (Mecina et al, 2014). Ouratea spectabilis is popularly known as "folhas de serra (pt) or sawblade leaves (in)", morphologically, it is an arboreal plant, presenting extrafloral, deciduous, heliophytic nectary and indifferent to soil conditions (Montesinos & Oliveira, 2014;Alves -Silva & Del-Claro, 2016).…”

Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves

“…Assays performed with ethanol extracts of O. spectabilis leaves and bark showed antiviral activity against human herpes virus type 1 (HSV-1) and inhibitory activity against the production of TNF-α in monocytic cells activated by lipopolysaccharide (LPS) THP-1 (Brandão et al 2011;Campana et al 2015). Furthermore, the hydroethanolic extract and the ethyl acetate fraction of O. spectabilis showed phytotoxic activity, causing a decrease in the germination rate of lettuce seeds (Mecina et al 2014).…”