PI 296341, a Source of Resistance in Watermelon to Race 2 of Fusarium oxysporum f. sp. niveum

Netzer, D.; Martyn, R. D.

doi:10.1094/pd-73-0518e

Cited by 33 publications

(22 citation statements)

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“…Resistance to Fon race 1 is widely described in Calhoun Gray and is thought to be controlled by a single dominant gene designated Fo-1 with a few modifier genes (Guner and Wehner, 2004;Martyn and Netzer, 1991;Netzer and Weintall, 1980;Wehner 2008;Zhang and Rhodes, 1993). However, early efforts to identify loci associated with resistance to Fon race 1 primarily utilized the resistant PI 296341-FR (var.…”

Section: Introductionmentioning

confidence: 99%

Genotyping by sequencing for SNP discovery and genetic mapping of resistance to race 1 of Fusarium oxysporum in watermelon

Meru

McGregor

2016

Scientia Horticulturae

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a b s t r a c t Management of fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) (E.F. Sm.) W.C. Snyder & H.N. Han. in watermelon [Citrullus lanatus (Thunb.) Matsum.& Nakai] is largely dependent on cultivation of resistant cultivars. Application of marker-assisted selection (MAS) in conventional breeding programs can accelerate the release of new watermelon cultivars resistant to fusarium wilt. Towards developing tools for MAS, physical (1024 SNPs) and genetic (389 SNPs) maps were developed in the current study for an F 2 population (n = 89; Calhoun Gray x Sugar Baby) segregating for resistance against Fon race 1 using the genotyping by sequencing platform. A modified tray-dip method was established for high-throughput phenotyping of the segregating F 3 population. A major quantitative trait locus (QTL) accounting for up to 38.4% of the phenotypic variation in the F 3 population was identified on chromosome 1 on both the physical and genetic maps in a region previously associated with Fon race 1 resistance. This resistance locus was consistently detected over five different time points and three different phenotypic screens, showing the reliability of the screening method in discriminating susceptible and resistant genotypes. Eight resistance genes were found within the confidence interval of the identified QTL. SNPs close to this QTL may be exploited in MAS for fusarium wilt resistance in breeding programs. This study confirms the resistance locus on chromosome 1 and demonstrates the use of a physical map for QTL detection in watermelon. The SNPs reported here will be useful for future genetic studies in watermelon.

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Section: Introductionmentioning

confidence: 99%

Genotyping by sequencing for SNP discovery and genetic mapping of resistance to race 1 of Fusarium oxysporum in watermelon

Meru

McGregor

2016

Scientia Horticulturae

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“…The country of origin for Grif 160568, PI 270 563, PI 270564 is South Africa while it is Zimbabwe for PI 482342. Kar 26 which is called ''Pastéque a confiture'' obtained from France and PI 296341 was reported to be resistant to Fusarium wilt (Netzer and Martyn 1989) Only the accessions Kar 213 and Kar 209; both of which collected from the same region (Middle Anatolia) grouped together (61% bootstrap value). The cluster analysis indicated that there is no correlation between the grouping and the geographical origin of the accessions in agreement with results of morphological characterization reported by Solmaz and Sari (2009).…”

Section: Resultsmentioning

confidence: 99%

The genetic characterization of Turkish watermelon (Citrullus lanatus) accessions using RAPD markers

Solmaz

Sarı

Kaçar

et al. 2010

Genet Resour Crop Evol

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Genetic diversity of the Turkish watermelon genetic resources was evaluated using different Citrullus species, wild relatives, foreign landraces, open pollinated (OP) and commercial hybrid cultivars by RAPD markers. The germplasm was consisted of 303 accessions collected from various geographical regions. Twenty-two of 35 RAPD primers generated a total of 241 reproducible bands, 146 (60.6%) of which were polymorphic. Based on the RAPD data the genetic similarity coefficients were calculated and the dendrogram was constructed using UPGMA (Unweighted pair-group method with arithmetic average). Cluster analysis of the 303 accessions employing RAPD data resulted in a multi-branched dendrogram indicating that most of the Turkish accessions belonging to var. lanatus of Citrullus lanatus (Thunb.) Matsum et Nakai were grouped together. Accessions of different Citrullus species and Praecitrullus fistulosus (Stocks) Pangalo formed distant clusters from C. lanatus var. lanatus. Among 303 accessions, a subset of 56 accessions was selected representing different groups and a second dendrogram was constructed. The genetic similarity coefficients (GS) within the Turkish accessions were ranged from 0.76 to 1.00 with 0.94 average indicating that they are closely related. Taken together, our results indicated that low genetic variability exist among the watermelon genetic resources collected from Turkey contrary to their remarkable phenotypic diversity.

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“…Most watermelon cultivars developed in North America during the last 2 centuries have a narrow genetic background that may contribute to their elevated susceptibility to diseases and pests (Levi et al 2000(Levi et al , 2001a (Sowell and Pointer 1962;Netzer and Martyn 1989).…”

Section: Introductionmentioning

confidence: 99%

“…citroides; resistant to races 1 and 2 of F. oxysporum f. sp. niveum) (Netzer and Martyn 1989;Martyn and Netzer 1991) and NHM (C. lanatus var. lanatus; Fusarium wilt susceptible).…”

Section: Introductionmentioning

confidence: 99%

A genetic linkage map for watermelon derived from a testcross population: (Citrullus lanatus var. citroides × C. lanatus var. lanatus) × Citrullus colocynthis

et al. 2002

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A genetic linkage map was constructed for watermelon using a testcross population [Plant Accession Griffin 14113 ( Citrullus lanatus var. citroides) x New Hampshire Midget (NHM; C. lanatus var. lanatus)] x U.S. Plant Introduction (PI) 386015 ( Citrullus colocynthis). The map contains 141 randomly amplified polymorphic DNA (RAPD) markers produced by 78 primers, 27 inter-simple sequence repeat (ISSR) markers produced by 17 primers, and a sequence-characterized amplified region (SCAR) marker that was previously reported as linked (1.6 cM) to race-1 Fusarium wilt [incited by Fusarium oxysporum Schlechtend.:Fr. f. sp. niveum (E.F.Sm.) W.C. Synder & H.N. Hans] resistance in watermelon. The map consists of 25 linkage groups. Among them are a large linkage group that contains 22 markers covering a mapping distance of 225.6 cM and six large groups each with 10-20 markers covering a mapping distance of 68.8 to 110.8 cM. There are five additional linkage groups consisting of 3-7 markers per group, each covering a mapping distance of 36.5 to 57.2 cM. The 13 remaining linkage groups are small, each consisting of 2-11 markers covering a mapping distance of 3.5-29.9 cM. The entire map covers a total distance of 1,166.2 cM with an average distance of 8.1 cM between two markers. This map is useful for the further development of markers linked to disease resistance and watermelon fruit qualities.

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PI 296341, a Source of Resistance in Watermelon to Race 2 of Fusarium oxysporum f. sp. niveum

Cited by 33 publications

References 0 publications

Genotyping by sequencing for SNP discovery and genetic mapping of resistance to race 1 of Fusarium oxysporum in watermelon

Genotyping by sequencing for SNP discovery and genetic mapping of resistance to race 1 of Fusarium oxysporum in watermelon

The genetic characterization of Turkish watermelon (Citrullus lanatus) accessions using RAPD markers

A genetic linkage map for watermelon derived from a testcross population: (Citrullus lanatus var. citroides × C. lanatus var. lanatus) × Citrullus colocynthis

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