PI-PLCβ1 gene copy number alterations in breast cancer

Abstract

Abstract. Deregulation of signal transduction pathways frequently confers selective biological advantages to tumors. Phosphoinositides play an essential role in numerous cellular functions and, among the enzymes implicated in these processes, phosphoinositide-specific phospholipase C β1 (PI-PLCβ1) is one of the key regulators. In the present study, a fluorescence in situ hybridization (FISH) approach was used to investigate PI-PLCβ1 gene copy number alterations in various types of breast cancer differing in their invasiveness and proliferative activity, according to their mitotic index. At the molecular level, we also performed both real-time PCR and immunohistochemical analyses on PI-PLCβ1 to further investigate its expression in primary breast cancers. Finally, we analyzed the correlation between PI-PLCβ1 gene copy number and clinicopathological parameters. Our results show that most of our cases had aneusomies on the PI-PLCβ1 locus (20p12) and amplification of this specific region was the most frequent alteration observed. Our findings also indicate that the amplification of the region containing the PI-PLCβ1 gene was mostly related to the mitotic index, rather than to the invasion status. Finally, even though our case series is limited, PI-PLCβ1 gene amplification seems to be correlated to clinicopathological parameters.

Up-regulated expression of phospholipase C, β1 is associated with tumor cell proliferation and poor prognosis in hepatocellular carcinoma

“… 21 In breast cancer, gain of PLCB1 closely related to histological grade and proliferative index, and low expression of PLCB1 in luminal-like A type tumors which had a relatively good prognosis. 16 An earlier study found that a role for gains on chromosomes 8q and 20 as predictors of poor outcome in hepatoblastomas. 22 The PLCB1 gene is located at chromosome 20p12, which makes us concern its up-regulated status.…”

“… 14 More importantly, elevated level of PLCB1 in carcinogenesis has also been recently reported. 15 Molinari et al 16 demonstrated that PLCB1 gene copy number altered in breast cancer and the overexpression of PLCB1 was associated with histological grade and proliferative index.…”

HDAC8 Activates AKT through Upregulating PLCB1 and Suppressing DESC1 Expression in MEK1/2 Inhibition-Resistant Cells

“…Therefore, it is possible that signaling from GPCRs could confer survival benefits for cells expressing high levels of PLCB1. The high expression of PLCB1 was also shown to be related to the development and poor prognosis of various cancers, including hepatocarcinoma [ 43 , 44 , 45 ], colorectal cancers [ 46 , 47 ], non-small cell lung carcinoma [ 45 ], breast cancer [ 48 ], and acute myeloid leukemia [ 49 ], suggesting its oncogenic role in different cancers.…”

Capsazepine antagonizes TRPV1 activation induced by thermal and osmotic stimuli in human odontoblast-like cells

“… 13 β-actin was used as normalizing gene. 14 The amplification conditions were 95 °C for 3 min, 35 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s. The amplicons were separated and visualized on a 2% agarose gel stained with ethidium bromide.…”