PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles

Abstract

BackgroundIntermittent dehydration caused by tidal changes is one of the most important abiotic factors that intertidal seaweeds must cope with in order to retain normal growth and reproduction. However, the underlying molecular mechanisms for the adaptation of red seaweeds to repeated dehydration-rehydration cycles remain poorly understood.ResultsWe chose the red seaweed Gloiopeltis furcata as a model and simulated natural tidal changes with two consecutive dehydration-rehydration cycles occurring over 24 h in order to gain insight into key molecular pathways and regulation of genes which are associated with dehydration tolerance. Transcription sequencing assembled 32,681 uni-genes (GC content = 55.32%), of which 12,813 were annotated. Weighted gene co-expression network analysis (WGCNA) divided all transcripts into 20 modules, with Coral2 identified as the key module anchoring dehydration-induced genes. Pathways enriched analysis indicated that the ubiquitin-mediated proteolysis pathway (UPP) and phosphatidylinositol (PI) signaling system were crucial for a successful response in G. furcata. Network-establishing and quantitative reverse transcription PCR (qRT-PCR) suggested that genes encoding ubiquitin-protein ligase E3 (E3–1), SUMO-activating enzyme sub-unit 2 (SAE2), calmodulin (CaM) and inositol-1,3,4-trisphosphate 5/6-kinase (ITPK) were the hub genes which responded positively to two successive dehydration treatments. Network-based interactions with hub genes indicated that transcription factor (e.g. TFIID), RNA modification (e.g. DEAH) and osmotic adjustment (e.g. MIP, ABC1, Bam1) were related to these two pathways.ConclusionsRNA sequencing-based evidence from G. furcata enriched the informational database for intertidal red seaweeds which face periodic dehydration stress during the low tide period. This provided insights into an increased understanding of how ubiquitin-mediated proteolysis and the phosphatidylinositol signaling system help seaweeds responding to dehydration-rehydration cycles.

TMT-Based Proteomic Profiling Reveals the Molecular Mechanism in the Cold Tolerance of Rhododendron Aureum Georgi.

“…As we know, similar to drought stress, cold stress can also bring about the imbalance of plant water status. As an important hub gene for ubiquitin mediated proteolysis pathway, SUMO-activating enzyme subunit 2 (SEA2) plays an important role in avoiding excessive water loss during drought stress [13]. In our study, cold stress also induced the accumulation of SEA2, and the up-regulation of this protein may play a positive role in the adaptation of Rhododendron to severe cold (Table 1).…”

Effects of heat shock on photosynthesis-related characteristics and lipid profile of Cycas multipinnata and C. panzhihuaensis

“…5 ) might be beneficial to maintain the membrane stability or repair the damaged membrane. PI and PIP are the components of the PI signal system, which play key roles in the perception and transduction of environmental stimuli [ 81 ]. The significant increase of PI and PIP in C. multipinnata and the increase of PI in C. panzhihuaensis (Fig.…”

Effects of repeated drought stress on the physiological characteristics and lipid metabolism of Bombax ceiba L. during subsequent drought and heat stresses

“…The registered increase in DAG content in D3-treated plants may have been induced by previous repeated dehydration (Additional file 3), but whether the accumulated phospholipids were converted from the DAG induced during drought hardening needs verification. Among the classes of phospholipids, PI and PIP are components of the PI signal system involved in the perception and transduction of environmental stimuli [71]. The induction of PI and PIP by H treatment suggests that the PI signal pathway is involved in the response and adaptation of B. ceiba to sudden heat shock.…”