Piceatannol Exerts Anti-Obesity Effects in C57BL/6 Mice through Modulating Adipogenic Proteins and Gut Microbiota

Abstract

Obesity is a global health concern. Piceatannol (Pic), an analog of resveratrol (Res), has many reported biological activities. In this study, we investigated the anti-obesity effect of Pic in a high-fat diet (HFD)-induced obese animal model. The results showed that Pic significantly reduced mouse body weight in a dose-dependent manner without affecting food intake. Serum total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL) levels, and blood glucose (GLU) were significantly lowered in Pic-treated groups. Pic significantly decreased the weight of liver, spleen, perigonadal and retroperitoneal fat compared with the HFD group. Pic significantly reduced the adipocyte cell size of perigonadal fat and decreased the weight of liver. Pic-treated mice showed higher phosphorylated adenosine 5 -monophosphate-activated protein kinase (pAMPK) and phosphorylated acetyl-CoA carboxylase (pACC) protein levels and decreased protein levels of CCAAT/enhancer-binding protein C/EBPα, peroxisome proliferator-activated receptor PPARγ and fatty acid synthase (FAS), resulting in decreased lipid accumulation in adipocytes and the liver. Pic altered the composition of the gut microbiota by increasing Firmicutes and Lactobacillus and decreasing Bacteroidetes compared with the HFD group. Collectively, these results suggest that Pic may be a candidate for obesity treatment.

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“…Genomic DNA was extracted from fecal samples using the innuSPEED Stool DNA kit (Analytik Jena AG) and following manufacturer's instructions. The primer sequences to amplify the V4 region of 16S rDNA was obtained from a previous study and the PCR conditions were refered by our previously reported . Then, the second Index PCR using the Illumina DNA library preparation kit (Illumina, San Diego, CA, USA) to construct the dual‐index barcodes and sequencing adaptors under the following conditions: 95°C for 3 min, 8 cycles of 95°C (30 s), 55°C (30 s), and 72°C (30 s), with a final extension at 72°C for 5 min.…”

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